LAMA3 Antibodies

Structure of LAMA3

The LAMA3 gene encodes the laminin α3 chain, a large glycoprotein with a molecular weight of approximately 200 kDa. The actual molecular weight of this protein varies depending on different splicing variants.

This protein is composed of multiple domains and presents as an extended fibrous molecule. Its core functional domains include the spherical domains at the N-terminal (LN, LEa) and the G-domain at the C-terminal, which mediate specific binding to cell surface receptors such as integrins. The α3 chain of laminin covalently binds to the β3 and γ2 chains through its coiled helical region, jointly assembling into the biologically active laminin -332 trimer. This unique structure enables it to act as a bridge, bidirectionally connecting epithelial cells with other stromal components in the basement membrane, thereby playing a crucial role in anchoring and signal transduction.

Fig. 1 Lama3 interaction genes (PPI).¹

Key structural properties of LAMA3:

• Domains rich in epidermal growth factor-like (EGF-like) repeat sequences
• Form the C-terminal coiled coil region of the triple helix coil
• C-terminal laminin G-like domains that mediate cell adhesion

Functions of LAMA3

The LAMA3 gene encodes the laminin α3 chain, whose main function is to form and stabilize the basement membrane of epithelial tissues. However, it is also involved in a variety of complex physiological and pathological processes, including wound healing, cell signal transduction and tumor invasion.

Laminin -332 is assembled from three chains, α3, β3, and γ2, through covalent cross-linking in the coiled helical region. This unique heterotrimer structure determines its powerful network formation ability and biological function, which contrasts sharply with the oxygen storage function of myoglobin as an independent monomer.

Applications of LAMA3 and LAMA3 Antibody in Literature

1. Feng, Li-yuan, et al. "LAMA3 DNA methylation and transcriptome changes associated with chemotherapy resistance in ovarian cancer." Journal of ovarian research 14.1 (2021): 67. https://doi.org/10.1186/s13048-021-00807-y

This study confirmed through bioinformatics and experiments that hypermethylation of the LAMA3 gene leads to a decrease in its expression, which is associated with chemotherapy resistance and poor prognosis of ovarian cancer. LAMA3 can inhibit the proliferation of drug-resistant cells and promote their apoptosis, invasion and migration.

2. Islam, Kittiya, et al. "Upregulated LAMA3 modulates proliferation, adhesion, migration and epithelial‑to‑mesenchymal transition of cholangiocarcinoma cells." Scientific Reports 13.1 (2023): 22598. https://doi.org/10.1038/s41598-023-48798-8

Research has found that LAMA3 is the most significantly upregulated core laminin gene in cholangiocarcinoma, driving tumor proliferation, migration and invasion. LAMA3 has been revealed for the first time as a potential diagnostic marker and new therapeutic target for cholangiocarcinoma.

3. Ji, Zhong-Hao, et al. "A missense mutation in Lama3 causes androgen alopecia." Scientific Reports 13.1 (2023): 20818. https://doi.org/10.1038/s41598-023-48337-5

This study, through gene sequencing and CRISPR/Cas9 technology, confirmed that point mutations in the Lama3 gene (R217C) would cause mice to exhibit phenotypes similar to androgenetic alopecia, including hair loss and abnormal hair follicle structure. This mutant mouse model provides a new tool for the study of hair loss diseases.

4. Ullah, Asmat, Fibhaa Syed, and Shazia Khan. "c. 151dup variant in LAMA3 in Pakistani patients affected with Shabbir Syndrome but showing mild symptoms." Pakistan Journal of Medical Sciences 39.4 (2023): 1124. https://doi.org/10.12669/pjms.39.4.6926

This study found that a homozygous repeat mutation (c.151dup) of the LAMA3 gene in a Pakistani family is the cause of Shabbir syndrome. This mutation is relatively common among the Punjabi population in Pakistan. It is recommended to develop targeted detection technologies for this mutation to facilitate carrier screening and prenatal diagnosis.

5. Tian, Chengming, Xiyao Li, and Chunlin Ge. "High expression of LAMA3/AC245041. 2 gene pair associated with KRAS mutation and poor survival in pancreatic adenocarcinoma: a comprehensive TCGA analysis." Molecular Medicine 27.1 (2021): 62. https://doi.org/10.1186/s10020-021-00322-2

In this study, a prognostic model for KRAS-mutated pancreatic cancer was constructed. It was found that LAMA3 was highly correlated with the expression of lncRNA AC245041.2 and jointly participated in the regulatory network of transcription factors, providing new clues for exploring its molecular mechanism.

Creative Biolabs: LAMA3 Antibodies for Research

Creative Biolabs specializes in the production of high-quality LAMA3 antibodies for research and industrial applications. Our portfolio includes monoclonal antibodies tailored for ELISA, Flow Cytometry, Western blot, immunohistochemistry, and other diagnostic methodologies.

• Custom LAMA3 Antibody Development: Tailor-made solutions to meet specific research requirements.
• Bulk Production: Large-scale antibody manufacturing for industry partners.
• Technical Support: Expert consultation for protocol optimization and troubleshooting.
• Aliquoting Services: Conveniently sized aliquots for long-term storage and consistent experimental outcomes.

For more details on our LAMA3 antibodies, custom preparations, or technical support, contact us at email.