Rabbit Anti-ALOX5AP Recombinant Antibody (CBXF-1219) (CBMAB-F0750-CQ)

Name: Rabbit Anti-ALOX5AP Recombinant Antibody (CBXF-1219)
SKU: CBMAB-F0750-CQ
Rating: 5 (1 reviews)

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Published Data

Rabbit Anti-ALOX5AP Recombinant Antibody (CBXF-1219) in WB

FLAP expression levels in lung cell lines
(A) Western blot analysis of Beas2B (normal lung), A549 (NSCLC), H1299 (NSCLC), and H1975 (NSCLC) cell lysates. Western blots were repeated at least three times. (B) Quantification of relative FLAP protein levels was performed using the gel analysis tool on ImageJ software and normalized to α-tubulin protein levels. Analysis indicated significant overexpression of FLAP protein in A549 (lane 2) and H1299 cells (lane 3), but not H1975 cells (lane 4) with respect to Beas2B cells (lane 1). (*) P < 0.04, n=3. (C) ΔΔCT qRT-PCR analysis indicated significantly increased expression of FLAP mRNA in Beas2B cells compared to A549 and H1299 cells, but not H1975 cells. FLAP expression was normalized to GAPDH mRNA. (*) P < 0.01, n=3. ...View More

Iacona, J. R., Monteleone, N. J., & Lutz, C. S. (2018). miR-146a suppresses 5-lipoxygenase activating protein (FLAP) expression and Leukotriene B4 production in lung cancer cells. Oncotarget, 9(42), 26751.

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Rabbit

Clone

CBXF-1219

Application

Immunogen

Synthetic peptide within Human FLAP aa 100-200.

Host Species

Rabbit

Specificity

Human

Antibody Isotype

IgG

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:1,000-1:10,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Supernatant

Buffer

40% Glycerol, 9.85% Tris glycine

Preservative

0.05% sodium azide

Concentration

Batch dependent

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Arachidonate 5-Lipoxygenase Activating Protein

Introduction

This gene encodes a protein which, with 5-lipoxygenase, is required for leukotriene synthesis. Leukotrienes are arachidonic acid metabolites which have been implicated in various types of inflammatory responses, including asthma, arthritis and psoriasis. This protein localizes to the plasma membrane. Inhibitors of its function impede translocation of 5-lipoxygenase from the cytoplasm to the cell membrane and inhibit 5-lipoxygenase activation. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene.

Entrez Gene ID

241

UniProt ID

P20292

Alternative Names

Arachidonate 5-Lipoxygenase Activating Protein; MK-886-Binding Protein; Arachidonate 5-Lipoxygenase-Activating Protein; FLAP; Five-Lipoxygenase Activating Protein;

Function

Required for leukotriene biosynthesis by ALOX5 (5-lipoxygenase). Anchors ALOX5 to the membrane. Binds arachidonic acid, and could play an essential role in the transfer of arachidonic acid to ALOX5. Binds to MK-886, a compound that blocks the biosynthesis of leukotrienes.

Biological Process

Cellular response to calcium ion Source: UniProtKB
Leukotriene biosynthetic process Source: UniProtKB
Leukotriene metabolic process Source: Reactome
Lipoxin biosynthetic process Source: Reactome
Lipoxygenase pathway Source: Reactome
Protein homotrimerization Source: UniProtKB

Cellular Location

Nucleus membrane; Endoplasmic reticulum membrane

Involvement in disease

Ischemic stroke (ISCHSTR): A stroke is an acute neurologic event leading to death of neural tissue of the brain and resulting in loss of motor, sensory and/or cognitive function. Ischemic strokes, resulting from vascular occlusion, is considered to be a highly complex disease consisting of a group of heterogeneous disorders with multiple genetic and environmental risk factors.

Topology

Lumenal: 1-8 aa
Helical: 9-30 aa
Cytoplasmic: 31-52 aa
Helical: 53-77 aa
Lumenal: 78-80 aa
Helical: 81-102 aa
Cytoplasmic: 103-107 aa
Helical: 108-115 aa
Lumenal: 116-128 aa
Helical: 129-161 aa

Ye, X., An, L., Wang, X., Zhang, C., Huang, W., Sun, C., ... & Gao, M. (2021). ALOX5AP Predicts Poor Prognosis by Enhancing M2 Macrophages Polarization and Immunosuppression in Serous Ovarian Cancer Microenvironment. Frontiers in oncology, 11, 1811.

Mei, F., Wang, Y. F., Yang, D., Zuo, R. X., Shen, T., Yang, T. H., & Sa, Y. L. (2020). Relationship between Polymorphism in ALOX5, ALOX5AP and Susceptibility to Myeloid Leukemia. Zhongguo shi yan xue ye xue za zhi, 28(1), 40-50.

Zheng, J. H., Ning, G. L., Xu, W. H., Wu, X. C., & Ma, X. C. (2019). Lack of association between ALOX5AP genetic polymorphisms and risk of ischemic stroke: evidence from meta-analyses. Neuropsychiatric disease and treatment, 15, 357.

Ye, H., Zhang, X., Chen, Z., Li, X., Zhang, T., Yang, C., & Huang, L. (2018). Association between the polymorphism (rs17222919,− 1316T/G) of 5-lipoxygenase-activating protein gene (ALOX5AP) and the risk of stroke: A meta analysis. Medicine, 97(41).

Nunns, G. R., Stringham, J. R., Gamboni, F., Moore, E. E., Fragoso, M., Stettler, G. R., ... & Banerjee, A. (2018). Trauma and hemorrhagic shock activate molecular association of 5-lipoxygenase and 5-lipoxygenase–Activating protein in lung tissue. Journal of Surgical Research, 229, 262-270.

Balendran, C., Eriksson, N., Whatling, C., Walentinsson, A., Skrtic, S., Akerblom, A., ... & Wallentin, L. (2017). P3247Polymorphisms of the ALOX5AP gene (5-lipoxygenase activating protein) are associated with increased cardiovascular risk in patients with acute coronary syndrome: Analysis of PLATO genetics sub-study. European Heart Journal, 38(suppl_1).

Kowal-Bielecka, O., Chwiesko-Minarowska, S., Bernatowicz, P. L., Allanore, Y., Radstake, T., Matucci-Cerinic, M., ... & Kowal, K. (2017). The arachidonate 5-lipoxygenase activating protein gene polymorphism is associated with the risk of scleroderma-related interstitial lung disease: a multicentre European Scleroderma Trials and Research group (EUSTAR) study. Rheumatology, 56(5), 844-852.

Chen, Z., Zheng, J., Liu, W., Yang, K., Li, K., Huang, B., ... & Li, L. (2017). The SG13S114 polymorphism of the ALOX5AP gene is associated with ischemic stroke in Europeans: a meta-analysis of 8062 subjects. Neurological Sciences, 38(4), 579-587.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

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