Human ALOX5AP ELISA Kit (V2LY-0626-LY5893)

Name: Human ALOX5AP ELISA Kit
SKU: V2LY-0626-LY5893
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Sensitivity

0.0014 ng/mL

Detection Range

0.003-0.9 ng/mL

Sample Type

Serum, Plasma, cell culture supernates

Specificity

Human

Assay Type

Sandwich

Reactivity

Human

Assay Time

1.5 h

Molecule Mass

18.2 kDa

Components

Pre-coated ELISA Plate: 12 wells * 8 detachable strips
Standard solution: 0.5ml x1
Standard diluent: 3ml x1
Streptavidin-HRP: 6ml x1
Stop solution: 6ml x1
Substrate solution A: 6ml x1
Substrate solution B: 6ml x1
Wash buffer concentrate (25x): 20ml x1
Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage

Store at 2-8°C

More Infomation

Target

Full Name

Arachidonate 5-Lipoxygenase Activating Protein

Function

Required for leukotriene biosynthesis by ALOX5 (5-lipoxygenase). Anchors ALOX5 to the membrane. Binds arachidonic acid, and could play an essential role in the transfer of arachidonic acid to ALOX5. Binds to MK-886, a compound that blocks the biosynthesis of leukotrienes.

Biological Process

Cellular response to calcium ion Source: UniProtKB
Leukotriene biosynthetic process Source: UniProtKB
Leukotriene metabolic process Source: Reactome
Lipoxin biosynthetic process Source: Reactome
Lipoxygenase pathway Source: Reactome
Protein homotrimerization Source: UniProtKB

Cellular Location

Nucleus membrane; Endoplasmic reticulum membrane

Involvement in disease

Ischemic stroke (ISCHSTR): A stroke is an acute neurologic event leading to death of neural tissue of the brain and resulting in loss of motor, sensory and/or cognitive function. Ischemic strokes, resulting from vascular occlusion, is considered to be a highly complex disease consisting of a group of heterogeneous disorders with multiple genetic and environmental risk factors.

Topology

Lumenal: 1-8 aa
Helical: 9-30 aa
Cytoplasmic: 31-52 aa
Helical: 53-77 aa
Lumenal: 78-80 aa
Helical: 81-102 aa
Cytoplasmic: 103-107 aa
Helical: 108-115 aa
Lumenal: 116-128 aa
Helical: 129-161 aa

Ye, X., An, L., Wang, X., Zhang, C., Huang, W., Sun, C., ... & Gao, M. (2021). ALOX5AP Predicts Poor Prognosis by Enhancing M2 Macrophages Polarization and Immunosuppression in Serous Ovarian Cancer Microenvironment. Frontiers in oncology, 11, 1811.

Mei, F., Wang, Y. F., Yang, D., Zuo, R. X., Shen, T., Yang, T. H., & Sa, Y. L. (2020). Relationship between Polymorphism in ALOX5, ALOX5AP and Susceptibility to Myeloid Leukemia. Zhongguo shi yan xue ye xue za zhi, 28(1), 40-50.

Zheng, J. H., Ning, G. L., Xu, W. H., Wu, X. C., & Ma, X. C. (2019). Lack of association between ALOX5AP genetic polymorphisms and risk of ischemic stroke: evidence from meta-analyses. Neuropsychiatric disease and treatment, 15, 357.

Ye, H., Zhang, X., Chen, Z., Li, X., Zhang, T., Yang, C., & Huang, L. (2018). Association between the polymorphism (rs17222919,− 1316T/G) of 5-lipoxygenase-activating protein gene (ALOX5AP) and the risk of stroke: A meta analysis. Medicine, 97(41).

Nunns, G. R., Stringham, J. R., Gamboni, F., Moore, E. E., Fragoso, M., Stettler, G. R., ... & Banerjee, A. (2018). Trauma and hemorrhagic shock activate molecular association of 5-lipoxygenase and 5-lipoxygenase–Activating protein in lung tissue. Journal of Surgical Research, 229, 262-270.

Balendran, C., Eriksson, N., Whatling, C., Walentinsson, A., Skrtic, S., Akerblom, A., ... & Wallentin, L. (2017). P3247Polymorphisms of the ALOX5AP gene (5-lipoxygenase activating protein) are associated with increased cardiovascular risk in patients with acute coronary syndrome: Analysis of PLATO genetics sub-study. European Heart Journal, 38(suppl_1).

Kowal-Bielecka, O., Chwiesko-Minarowska, S., Bernatowicz, P. L., Allanore, Y., Radstake, T., Matucci-Cerinic, M., ... & Kowal, K. (2017). The arachidonate 5-lipoxygenase activating protein gene polymorphism is associated with the risk of scleroderma-related interstitial lung disease: a multicentre European Scleroderma Trials and Research group (EUSTAR) study. Rheumatology, 56(5), 844-852.

Chen, Z., Zheng, J., Liu, W., Yang, K., Li, K., Huang, B., ... & Li, L. (2017). The SG13S114 polymorphism of the ALOX5AP gene is associated with ischemic stroke in Europeans: a meta-analysis of 8062 subjects. Neurological Sciences, 38(4), 579-587.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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