NIFK

This gene encodes a protein that interacts with the forkhead-associated domain of the Ki-67 antigen. The encoded protein may bind RNA and may play a role in mitosis and cell cycle progression. Multiple pseudogenes exist on chromosomes 5, 10, 12, 15, and 19.

Full Name

NUCLEOLAR PROTEIN INTERACTING WITH THE FHA DOMAIN OF MKI67

Biological Process

Maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)Manual Assertion Based On ExperimentIBA:GO_Central
Protein-containing complex assembly1 PublicationNAS:UniProtKB
rRNA metabolic process1 PublicationNAS:UniProtKB
rRNA transcription1 PublicationNAS:UniProtKB

Cellular Location

Nucleus, nucleolus
Chromosome
Localizes to mitotic chromosomes in conjunction with MKI67.

PTM

Sequentially phosphorylated on Thr-238, Thr-234 and Ser-230. Thr-234 is phosphorylated only when Thr-238 is phosphorylated. Likewise, phosphorylation at Ser-230 requires that Thr-234 and Thr-238 are phosphorylated. Phosphorylation enhances MKI67 binding.

Anti-NIFK antibodies

Mouse Anti-NIFK Recombinant Antibody (CBWJN-0585) (CBMAB-N2465-WJ)

Datasheet

SDS

Target: NIFK

Host: Mouse

Antibody Isotype: IgG2a

Specificity: Human

Clone: CBWJN-0585

Application*: IH, IF

Mouse Anti-NIFK Recombinant Antibody (CBWJN-0584) (CBMAB-N2464-WJ)

Datasheet

SDS

Target: NIFK

Host: Mouse

Antibody Isotype: IgG1

Specificity: Human

Clone: CBWJN-0584

Application*: WB, IH

Mouse Anti-NIFK Recombinant Antibody (CBWJN-0551) (CBMAB-N2463-WJ)

Datasheet

SDS

Target: NIFK

Host: Mouse

Antibody Isotype: IgG1, κ

Specificity: Human

Clone: CBWJN-0551

Application*: WB

For Research Use Only. Not For Clinical Use.

(P): Predicted

* Abbreviations

IFImmunofluorescence

IHImmunohistochemistry

IPImmunoprecipitation

WBWestern Blot

EELISA

MMicroarray

CIChromatin Immunoprecipitation

FFlow Cytometry

FNFunction Assay

IDImmunodiffusion

RRadioimmunoassay

TCTissue Culture

GSGel Supershift

NNeutralization

BBlocking

AActivation

IInhibition

DDepletion

ESELISpot

DBDot Blot

MCMass Cytometry/CyTOF

CTCytotoxicity

SStimulation

AGAgonist

APApoptosis

IMImmunomicroscopy

BABioassay

CSCostimulation

EMElectron Microscopy

IEImmunoelectrophoresis

PAPeptide Array

ICImmunocytochemistry

PEPeptide ELISA

MDMeDIP

SHIn situ hybridization

IAEnzyme Immunoassay

SEsandwich ELISA

PLProximity Ligation Assay

ECELISA(Cap)

EDELISA(Det)

BIBioimaging

IOImmunoassay

LFLateral Flow Immunoassay

LALuminex Assay

CImmunohistochemistry-Frozen Sections

PImmunohistologyp-Paraffin Sections

ISIntracellular Staining for Flow Cytometry

MSElectrophoretic Mobility Shift Assay

RIRNA Binding Protein Immunoprecipitation (RIP)