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NIFK

This gene encodes a protein that interacts with the forkhead-associated domain of the Ki-67 antigen. The encoded protein may bind RNA and may play a role in mitosis and cell cycle progression. Multiple pseudogenes exist on chromosomes 5, 10, 12, 15, and 19.

Full Name

NUCLEOLAR PROTEIN INTERACTING WITH THE FHA DOMAIN OF MKI67

Biological Process

Maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)Manual Assertion Based On ExperimentIBA:GO_Central
Protein-containing complex assembly1 PublicationNAS:UniProtKB
rRNA metabolic process1 PublicationNAS:UniProtKB
rRNA transcription1 PublicationNAS:UniProtKB

Cellular Location

Nucleus, nucleolus
Chromosome
Localizes to mitotic chromosomes in conjunction with MKI67.

PTM

Sequentially phosphorylated on Thr-238, Thr-234 and Ser-230. Thr-234 is phosphorylated only when Thr-238 is phosphorylated. Likewise, phosphorylation at Ser-230 requires that Thr-234 and Thr-238 are phosphorylated. Phosphorylation enhances MKI67 binding.

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Anti-NIFK antibodies

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Mouse Anti-NIFK Recombinant Antibody (CBWJN-0585) (CBMAB-N2465-WJ)

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Target: NIFK

Host: Mouse

Antibody Isotype: IgG2a

Specificity: Human

Clone: CBWJN-0585

Application*: IH, IF

Mouse Anti-NIFK Recombinant Antibody (CBWJN-0584) (CBMAB-N2464-WJ)

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Target: NIFK

Host: Mouse

Antibody Isotype: IgG1

Specificity: Human

Clone: CBWJN-0584

Application*: WB, IH

Mouse Anti-NIFK Recombinant Antibody (CBWJN-0551) (CBMAB-N2463-WJ)

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Target: NIFK

Host: Mouse

Antibody Isotype: IgG1, κ

Specificity: Human

Clone: CBWJN-0551

Application*: WB

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Fig.3 Signaling pathways in cancers. (Creative Biolabs Authorized)

Fig.4 Protocols troubleshootings & guides. (Creative Biolabs Authorized)

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Fig.3 Signaling pathways in cancers. (Creative Biolabs Authorized)

Fig.4 Protocols troubleshootings & guides. (Creative Biolabs Authorized)

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For Research Use Only. Not For Clinical Use.

(P): Predicted

* Abbreviations

AActivation
AGAgonist
APApoptosis
BBlocking
BABioassay
BIBioimaging
CImmunohistochemistry-Frozen Sections
CIChromatin Immunoprecipitation
CTCytotoxicity
CSCostimulation
DDepletion
DBDot Blot

EELISA
ECELISA(Cap)
EDELISA(Det)
ESELISpot
EMElectron Microscopy
FFlow Cytometry
FNFunction Assay
GSGel Supershift
IInhibition
IAEnzyme Immunoassay
ICImmunocytochemistry
IDImmunodiffusion
IEImmunoelectrophoresis

IFImmunofluorescence
IGImmunochromatography
IHImmunohistochemistry
IMImmunomicroscopy
IOImmunoassay
IPImmunoprecipitation
ISIntracellular Staining for Flow Cytometry
LALuminex Assay
LFLateral Flow Immunoassay
MMicroarray
MCMass Cytometry/CyTOF
MDMeDIP

MSElectrophoretic Mobility Shift Assay
NNeutralization
PImmunohistologyp-Paraffin Sections
PAPeptide Array
PEPeptide ELISA
PLProximity Ligation Assay
RRadioimmunoassay
SStimulation
SESandwich ELISA
SHIn situ hybridization
TCTissue Culture
WBWestern Blot

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