Mouse Anti-CA9 Recombinant Antibody (CBXC-2079) (CBMAB-C0131-CQ)

Name: Mouse Anti-CA9 Recombinant Antibody (CBXC-2079)
SKU: CBMAB-C0131-CQ
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-CA9 Recombinant Antibody (CBXC-2079) (CBMAB-C0131-CQ) in IF

3D-tumouroid co-cultures were grown until different time points (days: 6, 10, 13, 20 and 31) and then the FFPE blocks of these 3D-tumouroid co-cultures were prepared. From these FFPE blocks, sections were stained with CAIX to observe the progression of hypoxia. Stained images were captured using Leica SPE confocal microscope under 10X objective with zoom factor 2. Scale bar: 20 µM. Composite images are CAIX (red) and nucleus (blue) and it was observed that the hypoxic-rich environment started to develop from day 10 and persisted until day 31. ...View More

Saha, S., Howarth, R., Sharma-Saha, S., & Kelly, C. (2023). Bioengineering of a tumour-stroma 3D-tumouroid co-culture model of hypopharyngeal cancer. Biology Open, 12(5), bio059949.

Mouse Anti-CA9 Recombinant Antibody (CBXC-2079) (CBMAB-C0131-CQ) in Immunoblot

Breast cancer cell lines were collected from the monolayer (D0) following 7 days of suspension culture (D7) and analyzed using immunoblotting for markers identified in the top dysregulated cell surface proteins as well as HIF1α. Carbonic Anhydrase IX (CAIX) and ENO2 were significantly upregulated following suspension culture in the cell lines studied. ...View More

Twomey, J. D., & Zhang, B. (2023). Exploring the Role of Hypoxia-Inducible Carbonic Anhydrase IX (CAIX) in Circulating Tumor Cells (CTCs) of Breast Cancer. Biomedicines, 11(3), 934.

Mouse Anti-CA9 Recombinant Antibody (CBXC-2079) (CBMAB-C0131-CQ) in IF

Representative immunofluorescence images of PDOs stained with typical markers of clear cell renal cancers, e.g., CD10 (green) and carbonic anhydrase 9 (red) Ki67 (pink). Nuclei were stained with 4',6-diamidino-2-phenylindole (DAPI) (blue). Scale bars = 100 μm or 20 μm. ...View More

Rupert, C., Dell'Aversana, C., Mosca, L., Montanaro, V., Arcaniolo, D., De Sio, M., ... & de Nigris, F. (2023). Therapeutic targeting of P2X4 receptor and mitochondrial metabolism in clear cell renal carcinoma models. Journal of Experimental & Clinical Cancer Research, 42(1), 134.

Mouse Anti-CA9 Recombinant Antibody (CBXC-2079) (CBMAB-C0131-CQ) in Immunoblot

NDRG1 and CA9 protein expression were analyzed with an immunoblot assay using lysate extracted from MDA-MB-231 and SUM159 cell lines treated with increasing doses of MBZ as indicated. ...View More

Joe, N. S., Wang, Y., Oza, H. H., Godet, I., Milki, N., Riggins, G. J., & Gilkes, D. M. (2023). Mebendazole Treatment Disrupts the Transcriptional Activity of Hypoxia-Inducible Factors 1 and 2 in Breast Cancer Cells. Cancers, 15(4), 1330.

Mouse Anti-CA9 Recombinant Antibody (CBXC-2079) (CBMAB-C0131-CQ) in IF

Representative images of immunofluorescence staining were obtained from both SAT and VAT samples showing CAIII and CAIX expression in both lean and obese patients as red and green signals, respectively. Of note, the higher dimension of the adipocytes in AT from obese vs. lean subjects suggest pathological differences. DAPI staining (blue) was used to mark cell nuclei. Scale bar = 50 µm. ...View More

Fei, L., Cantini, G., Nocentini, A., Nardini, P., Catarinicchia, S., Canu, L., ... & Luconi, M. (2023). Carbonic anhydrases III and IX are new players in the crosstalk between adrenocortical carcinoma and its altered adipose microenvironment. Journal of Endocrinological Investigation, 46(7), 1449-1458.

Mouse Anti-CA9 Recombinant Antibody (CBXC-2079) (CBMAB-C0131-CQ) in WB

Effects of BaP on CAIX, VEGF, CYP1A1 and CYP1B1 expression at the protein level without (□) or with (■) CoCl2 and with 0-8 μM BaP. Data are presented as the fold-increase compared to without B(α) P and CoCl2, * P < 0.05, ** P < 0.01. Without (□) or with (■) CoCl2 and with 0-8 μM BaP. Data are presented as the fold-increase compared to without B(α) P and CoCl2, * P < 0.05, ** P < 0.01, n = 3. ...View More

Zhang, M., Hu, Y., Yang, F., Zhang, J., Zhang, J., Yu, W., ... & Chang, F. (2022). Interaction between AhR and HIF-1 signaling pathways mediated by ARNT/HIF-1β. BMC Pharmacology and Toxicology, 23(1), 26.

Mouse Anti-CA9 Recombinant Antibody (CBXC-2079) (CBMAB-C0131-CQ) in IHC

Representative images of αvβ6 (A-E), CAIX (F-J) expression in healthy vulvar tissue with (sebaceous) glands, differentiated vulvar intraepithelial neoplasia (dVIN), human papilloma virus-independent vulvar squamous cell carcinoma (HPV-independent VSCC), high grade squamous intraepithelial lesion (HSIL) and human papilloma virus-dependent vulvar squamous cell carcinoma (HPV-dependent VSCC). All images show only the predefined tissue type of that section (no adjacent tissue). Scale bars represent 500 µm. ...View More

Huisman, B. W., Cankat, M., Bosse, T., Vahrmeijer, A. L., Rissmann, R., Burggraaf, J., ... & van Poelgeest, M. I. (2021). Integrin αvβ6 as a target for tumor-specific imaging of vulvar squamous cell carcinoma and adjacent premalignant lesions. Cancers, 13(23), 6006.

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBXC-2079

Application

E, WB, IF, IP, P

Immunogen

Amino acids 41-160 of CA IX of human origin.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500
ELISA	1:100-1:1,000
IHC-P	1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

0.09% sodium azide

Concentration

0.2 mg/ml

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

carbonic anhydrase 9

Introduction

Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. CA IX is a transmembrane protein and is one of only two tumor-associated carbonic anhydrase isoenzymes known. It is expressed in all clear-cell renal cell carcinoma, but is not detected in normal kidney or most other normal tissues. It may be involved in cell proliferation and transformation. This gene was mapped to 17q21.2 by fluorescence in situ hybridization, however, radiation hybrid mapping localized it to 9p13-p12.

Entrez Gene ID

768

UniProt ID

Q16790

Alternative Names

CAIX; MN

Function

Reversible hydration of carbon dioxide. Participates in pH regulation. May be involved in the control of cell proliferation and transformation. Appears to be a novel specific biomarker for a cervical neoplasia.

Biological Process

Bicarbonate transport Source: Reactome
Morphogenesis of an epithelium Source: Ensembl
One-carbon metabolic process Source: GO_Central
Regulation of transcription from RNA polymerase II promoter in response to hypoxia Source: Reactome
Response to drug Source: Ensembl
Response to testosterone Source: Ensembl
Secretion Source: Ensembl

Cellular Location

Nucleus; Nucleolus; Cell membrane; Microvillus membrane. Found on the surface microvilli and in the nucleus, particularly in nucleolus.

Topology

Extracellular: 38-414 aa
Helical: 415-435 aa
Cytoplasmic: 436-459 aa

PTM

Asn-346 bears high-mannose type glycan structures.

Giatromanolaki, A., Harris, A. L., Banham, A. H., Contrafouris, C. A., & Koukourakis, M. I. (2020). Carbonic anhydrase 9 (CA9) expression in non-small-cell lung cancer: correlation with regulatory FOXP3+ T-cell tumour stroma infiltration. British journal of cancer, 122(8), 1205-1210.

Baniak, N., Flood, T. A., Buchanan, M., Dal Cin, P., & Hirsch, M. S. (2020). Carbonic anhydrase IX (CA9) expression in multiple renal epithelial tumour subtypes. Histopathology, 77(4), 659-666.

Wang, S., Fu, Z., Wang, Y., Sun, Y., Cui, L., Wang, C., ... & Wen, N. (2020). Correlation of carbonic anhydrase 9 (CA9) with pathological T-stage and prognosis in patients with oral tongue squamous cell carcinoma. Annals of Translational Medicine, 8(22).

Li, Z., Jiang, L., Chew, S. H., Hirayama, T., Sekido, Y., & Toyokuni, S. (2019). Carbonic anhydrase 9 confers resistance to ferroptosis/apoptosis in malignant mesothelioma under hypoxia. Redox biology, 26, 101297.

Eckert, A. W., Horter, S., Bethmann, D., Kotrba, J., Kaune, T., Rot, S., ... & Kappler, M. (2019). Investigation of the prognostic role of carbonic anhydrase 9 (CAIX) of the cellular mRNA/protein level or soluble CAIX protein in patients with oral squamous cell carcinoma. International journal of molecular sciences, 20(2), 375.

McDonald, P. C., Chafe, S. C., Brown, W. S., Saberi, S., Swayampakula, M., Venkateswaran, G., ... & Dedhar, S. (2019). Regulation of pH by carbonic anhydrase 9 mediates survival of pancreatic cancer cells with activated KRAS in response to hypoxia. Gastroenterology, 157(3), 823-837.

Finkelmeier, F., Canli, Ö., Peiffer, K. H., Walter, D., Tal, A., Koch, C., ... & Waidmann, O. (2018). Circulating hypoxia marker carbonic anhydrase IX (CA9) in patients with hepatocellular carcinoma and patients with cirrhosis. PloS one, 13(7), e0200855.

Kim, J. Y., Lee, S. H., An, S., Kim, S. J., Sung, Y. N., Song, K. B., ... & Hong, S. M. (2018). Carbonic anhydrase 9 expression in well-differentiated pancreatic neuroendocrine neoplasms might be associated with aggressive behavior and poor survival. Virchows Archiv, 472(5), 739-748.

Horie, K., Kawakami, K., Fujita, Y., Sugaya, M., Kameyama, K., Mizutani, K., ... & Ito, M. (2017). Exosomes expressing carbonic anhydrase 9 promote angiogenesis. Biochemical and biophysical research communications, 492(3), 356-361.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Antibody Pairs

CA9 Matched Antibody Pair (165) (CAT#: APMAB-165LY)

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Isotype control

For research use only. Not intended for any clinical use.

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We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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