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Mouse Anti-ATP1B1 Recombinant Antibody (E4) (CBMAB-0463-LY)

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Published Data
Mouse Anti-ATP1B1 Recombinant Antibody (E4) (CBMAB-0463-LY) in IF
Mouse Anti-ATP1B1 Recombinant Antibody (E4) (CBMAB-0463-LY) in IF
Sarcolemmal localization of HSP60 and its interactions with TLR2 and TLR4 in myocardial tissue of subjects with left ventricular hypertrophy (LVH). (A-C) Representative photomicrographs show double immunofluorescence staining with anti-HSP60 (green staining) and anti-Naþ/Kþ-ATPase (red staining) antibodies on paraffin-embedded sections of the hypertrophic myocardium (cross-section of cardiomyocyte bundles). Blue marks DAPI staining of nuclei. Arrows indicate sites of HSP60 and Naþ/Kþ-ATPase co-localization (orange-yellowish coloration). Magnification 400. (D-I) Representative photomicrographs obtained by proximity ligation assay (PLA) on paraffin-embedded sections of the hypertrophic myocardium using anti-HSP60 antibody in combination with anti-TLR2 antibody (D-F) and anti-TLR4 antibody (G-I). Red fluorescent signals denote interaction sites in myocardial tissue. Blue marks DAPI staining of nuclei. Magnification 400. ...View More
Ferenčić, A., Cuculić, D., Stemberga, V., Šešo, B., Arbanas, S., & Jakovac, H. (2020). Left ventricular hypertrophy is associated with overexpression of HSP60, TLR2, and TLR4 in the myocardium. Scandinavian Journal of Clinical and Laboratory Investigation, 80(3), 236-246.
Datasheet
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Basic Information

Host Animal
Mouse
Clone
E4
Application
WB, IP, IF, ELISA, IHC-P
Immunogen
Amino acids 41-155 of Na+/K+-ATPase β1 of human origin.
Specificity
Human
Antibody Isotype
IgG1, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)1:50-1:500
IHC-P1:50-1:500
ELISA1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, 0.1% gelatin
Preservative
< 0.1% sodium azide
Concentration
0.2 mg/ml
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.
More Infomation

Target

Full Name
ATPase Na+/K+ Transporting Subunit Beta 1
Introduction
This is the non-catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of Na+ and K+ ions across the plasma membrane. The beta subunit regulates, through assembly of alpha/beta heterodimers, the number of sodium pumps transported to the plasma membrane.
Entrez Gene ID
396529
UniProt ID
P08251
Function
This is the non-catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of Na+ and K+ ions across the plasma membrane. The beta subunit regulates, through assembly of alpha/beta heterodimers, the number of sodium pumps transported to the plasma membrane.
Biological Process
ATP metabolic process Source: BHF-UCL
Cardiac muscle contraction Source: BHF-UCL
Cation transmembrane transport Source: ARUK-UCL
Cell adhesion Source: UniProtKB-KW
Cell communication by electrical coupling involved in cardiac conduction Source: BHF-UCL
Cellular calcium ion homeostasis Source: BHF-UCL
Cellular potassium ion homeostasis Source: BHF-UCL
Cellular sodium ion homeostasis Source: BHF-UCL
Establishment or maintenance of transmembrane electrochemical gradient Source: BHF-UCL
Ion transmembrane transport Source: Reactome
Leukocyte migration Source: Reactome
Membrane repolarization Source: BHF-UCL
Membrane repolarization during cardiac muscle cell action potential Source: BHF-UCL
Positive regulation of ATPase activity Source: BHF-UCL
Positive regulation of calcium:sodium antiporter activity Source: BHF-UCL
Positive regulation of potassium ion import across plasma membrane Source: BHF-UCL
Positive regulation of potassium ion transmembrane transporter activity Source: BHF-UCL
Positive regulation of P-type sodium:potassium-exchanging transporter activity Source: ARUK-UCL
Positive regulation of sodium ion export across plasma membrane Source: BHF-UCL
Potassium ion import across plasma membrane Source: ARUK-UCL
Protein localization to plasma membrane Source: BHF-UCL
Protein stabilization Source: BHF-UCL
Protein transport into plasma membrane raft Source: BHF-UCL
Regulation of cardiac conduction Source: Reactome
Regulation of cardiac muscle contraction by calcium ion signaling Source: BHF-UCL
Regulation of gene expression Source: BHF-UCL
Relaxation of cardiac muscle Source: BHF-UCL
Response to hypoxia Source: Ensembl
Sodium ion export across plasma membrane Source: BHF-UCL
Sodium ion transmembrane transport Source: ARUK-UCL
Cellular Location
Cell membrane; Apical cell membrane. Colocalizes with OBSCN at the intercalated disk and sarcolemma in cardiomyocytes. Localizes in long striations at the level of Z and M lines.
Topology
Cytoplasmic: 1-34 aa
Helical: 35-62 aa
Extracellular: 63-303 aa
PTM
Glutathionylated (By similarity). N-glycosylated (By similarity).
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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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For research use only. Not intended for any clinical use.

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We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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