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Mouse Anti-ATP1B1 Recombinant Antibody (E4) (CBMAB-0463-LY)

This product is mouse antibody recognizes ATP1B1 of chicken. The antibody b29 immunoassay techniques such as: IF, IP, WB.
See all ATP1B1 antibodies
Published Data
Mouse Anti-ATP1B1 Recombinant Antibody (E4) (CBMAB-0463-LY) in IF
Sarcolemmal localization of HSP60 and its interactions with TLR2 and TLR4 in myocardial tissue of subjects with left ventricular hypertrophy (LVH). (A-C) Representative photomicrographs show double immunofluorescence staining with anti-HSP60 (green staining) and anti-Naþ/Kþ-ATPase (red staining) antibodies on paraffin-embedded sections of the hypertrophic myocardium (cross-section of cardiomyocyte bundles). Blue marks DAPI staining of nuclei. Arrows indicate sites of HSP60 and Naþ/Kþ-ATPase co-localization (orange-yellowish coloration). Magnification 400. (D-I) Representative photomicrographs obtained by proximity ligation assay (PLA) on paraffin-embedded sections of the hypertrophic myocardium using anti-HSP60 antibody in combination with anti-TLR2 antibody (D-F) and anti-TLR4 antibody (G-I). Red fluorescent signals denote interaction sites in myocardial tissue. Blue marks DAPI staining of nuclei. Magnification 400. ...View More
Ferenčić, A., Cuculić, D., Stemberga, V., Šešo, B., Arbanas, S., & Jakovac, H. (2020). Left ventricular hypertrophy is associated with overexpression of HSP60, TLR2, and TLR4 in the myocardium. Scandinavian Journal of Clinical and Laboratory Investigation, 80(3), 236-246.

Summary

Host Animal
Mouse
Specificity
Human
Clone
E4
Antibody Isotype
IgG1, κ
Application
WB, IP, IF, ELISA, IHC-P

Basic Information

Immunogen
Amino acids 41-155 of Na+/K+-ATPase β1 of human origin.
Specificity
Human
Antibody Isotype
IgG1, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)1:50-1:500
IHC-P1:50-1:500
ELISA1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, 0.1% gelatin
Preservative
< 0.1% sodium azide
Concentration
0.2 mg/ml
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
ATPase Na+/K+ Transporting Subunit Beta 1
Introduction
This is the non-catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of Na+ and K+ ions across the plasma membrane. The beta subunit regulates, through assembly of alpha/beta heterodimers, the number of sodium pumps transported to the plasma membrane.
Entrez Gene ID
396529
UniProt ID
P08251
Function
This is the non-catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of Na+ and K+ ions across the plasma membrane. The beta subunit regulates, through assembly of alpha/beta heterodimers, the number of sodium pumps transported to the plasma membrane.
Biological Process
ATP metabolic process Source: BHF-UCL
Cardiac muscle contraction Source: BHF-UCL
Cation transmembrane transport Source: ARUK-UCL
Cell adhesion Source: UniProtKB-KW
Cell communication by electrical coupling involved in cardiac conduction Source: BHF-UCL
Cellular calcium ion homeostasis Source: BHF-UCL
Cellular potassium ion homeostasis Source: BHF-UCL
Cellular sodium ion homeostasis Source: BHF-UCL
Establishment or maintenance of transmembrane electrochemical gradient Source: BHF-UCL
Ion transmembrane transport Source: Reactome
Leukocyte migration Source: Reactome
Membrane repolarization Source: BHF-UCL
Membrane repolarization during cardiac muscle cell action potential Source: BHF-UCL
Positive regulation of ATPase activity Source: BHF-UCL
Positive regulation of calcium:sodium antiporter activity Source: BHF-UCL
Positive regulation of potassium ion import across plasma membrane Source: BHF-UCL
Positive regulation of potassium ion transmembrane transporter activity Source: BHF-UCL
Positive regulation of P-type sodium:potassium-exchanging transporter activity Source: ARUK-UCL
Positive regulation of sodium ion export across plasma membrane Source: BHF-UCL
Potassium ion import across plasma membrane Source: ARUK-UCL
Protein localization to plasma membrane Source: BHF-UCL
Protein stabilization Source: BHF-UCL
Protein transport into plasma membrane raft Source: BHF-UCL
Regulation of cardiac conduction Source: Reactome
Regulation of cardiac muscle contraction by calcium ion signaling Source: BHF-UCL
Regulation of gene expression Source: BHF-UCL
Relaxation of cardiac muscle Source: BHF-UCL
Response to hypoxia Source: Ensembl
Sodium ion export across plasma membrane Source: BHF-UCL
Sodium ion transmembrane transport Source: ARUK-UCL
Cellular Location
Cell membrane; Apical cell membrane. Colocalizes with OBSCN at the intercalated disk and sarcolemma in cardiomyocytes. Localizes in long striations at the level of Z and M lines.
Topology
Cytoplasmic: 1-34 aa
Helical: 35-62 aa
Extracellular: 63-303 aa
PTM
Glutathionylated (By similarity). N-glycosylated (By similarity).
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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