Human Recombinant ATP1B1 protein, His Tag (V2LY-0526-LY2200)

Name: Human Recombinant ATP1B1 protein, His Tag
SKU: V2LY-0526-LY2200
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

HEK293 Cells

Protein Species

Human

Tag

His Tag

Protein Construction

This product is Human Recombinant ATP1B1 protein, His Tag consist of Amino Acid: 63-303 and predicts a molecular mass of 30.4 kDa.

Molecule Mass

30.4 kDa

Sequence

Amino Acid: 63-303

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>95% as determined by SDS-PAGE

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile PBS

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

ATPase Na+/K+ Transporting Subunit Beta 1

Function

This is the non-catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of Na+ and K+ ions across the plasma membrane. The beta subunit regulates, through assembly of alpha/beta heterodimers, the number of sodium pumps transported to the plasma membrane.

Biological Process

ATP metabolic process Source: BHF-UCL
Cardiac muscle contraction Source: BHF-UCL
Cation transmembrane transport Source: ARUK-UCL
Cell adhesion Source: UniProtKB-KW
Cell communication by electrical coupling involved in cardiac conduction Source: BHF-UCL
Cellular calcium ion homeostasis Source: BHF-UCL
Cellular potassium ion homeostasis Source: BHF-UCL
Cellular sodium ion homeostasis Source: BHF-UCL
Establishment or maintenance of transmembrane electrochemical gradient Source: BHF-UCL
Ion transmembrane transport Source: Reactome
Leukocyte migration Source: Reactome
Membrane repolarization Source: BHF-UCL
Membrane repolarization during cardiac muscle cell action potential Source: BHF-UCL
Positive regulation of ATPase activity Source: BHF-UCL
Positive regulation of calcium:sodium antiporter activity Source: BHF-UCL
Positive regulation of potassium ion import across plasma membrane Source: BHF-UCL
Positive regulation of potassium ion transmembrane transporter activity Source: BHF-UCL
Positive regulation of P-type sodium:potassium-exchanging transporter activity Source: ARUK-UCL
Positive regulation of sodium ion export across plasma membrane Source: BHF-UCL
Potassium ion import across plasma membrane Source: ARUK-UCL
Protein localization to plasma membrane Source: BHF-UCL
Protein stabilization Source: BHF-UCL
Protein transport into plasma membrane raft Source: BHF-UCL
Regulation of cardiac conduction Source: Reactome
Regulation of cardiac muscle contraction by calcium ion signaling Source: BHF-UCL
Regulation of gene expression Source: BHF-UCL
Relaxation of cardiac muscle Source: BHF-UCL
Response to hypoxia Source: Ensembl
Sodium ion export across plasma membrane Source: BHF-UCL
Sodium ion transmembrane transport Source: ARUK-UCL

Cellular Location

Cell membrane; Apical cell membrane. Colocalizes with OBSCN at the intercalated disk and sarcolemma in cardiomyocytes. Localizes in long striations at the level of Z and M lines.

Topology

Cytoplasmic: 1-34 aa
Helical: 35-62 aa
Extracellular: 63-303 aa

PTM

Glutathionylated (By similarity). N-glycosylated (By similarity).

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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