PRKCI

This gene encodes a member of the protein kinase C (PKC) family of serine/threonine protein kinases. The PKC family comprises at least eight members, which are differentially expressed and are involved in a wide variety of cellular processes. This protein kinase is calcium-independent and phospholipid-dependent. It is not activated by phorbolesters or diacylglycerol. This kinase can be recruited to vesicle tubular clusters (VTCs) by direct interaction with the small GTPase RAB2, where this kinase phosphorylates glyceraldehyde-3-phosphate dehydrogenase (GAPD/GAPDH) and plays a role in microtubule dynamics in the early secretory pathway. This kinase is found to be necessary for BCL-ABL-mediated resistance to drug-induced apoptosis and therefore protects leukemia cells against drug-induced apoptosis. There is a single exon pseudogene mapped on chromosome X. [provided by RefSeq]

protein kinase C, iota

Calcium- and diacylglycerol-independent serine/ threonine-protein kinase that plays a general protective role against apoptotic stimuli, is involved in NF-kappa-B activation, cell survival, differentiation and polarity, and contributes to the regulation of microtubule dynamics in the early secretory pathway. Is necessary for BCR-ABL oncogene-mediated resistance to apoptotic drug in leukemia cells, protecting leukemia cells against drug-induced apoptosis. In cultured neurons, prevents amyloid beta protein-induced apoptosis by interrupting cell death process at a very early step. In glioblastoma cells, may function downstream of phosphatidylinositol 3-kinase (PI3K) and PDPK1 in the promotion of cell survival by phosphorylating and inhibiting the pro-apoptotic factor BAD. Can form a protein complex in non-small cell lung cancer (NSCLC) cells with PARD6A and ECT2 and regulate ECT2 oncogenic activity by phosphorylation, which in turn promotes transformed growth and invasion. In response to nerve growth factor (NGF), acts downstream of SRC to phosphorylate and activate IRAK1, allowing the subsequent activation of NF-kappa-B and neuronal cell survival. Functions in the organization of the apical domain in epithelial cells by phosphorylating EZR. This step is crucial for activation and normal distribution of EZR at the early stages of intestinal epithelial cell differentiation. Forms a protein complex with LLGL1 and PARD6B independently of PARD3 to regulate epithelial cell polarity. Plays a role in microtubule dynamics in the early secretory pathway through interaction with RAB2A and GAPDH and recruitment to vesicular tubular clusters (VTCs). In human coronary artery endothelial cells (HCAEC), is activated by saturated fatty acids and mediates lipid-induced apoptosis. Involved in early synaptic long term potentiation phase in CA1 hippocampal cells and short term memory formation (By similarity).

Actin filament organizationIEA:Ensembl
Cell migrationIEA:Ensembl
Cell-cell junction organizationManual Assertion Based On ExperimentIMP:UniProtKB
Cellular response to insulin stimulusISS:BHF-UCL
Cytoskeleton organization1 PublicationNAS:UniProtKB
Establishment of apical/basal cell polarityIEA:Ensembl
Establishment or maintenance of epithelial cell apical/basal polarityManual Assertion Based On ExperimentIDA:ComplexPortal
Eye photoreceptor cell developmentIEA:Ensembl
Golgi vesicle buddingIEA:Ensembl
Intracellular signal transductionManual Assertion Based On ExperimentIBA:GO_Central
Membrane organization1 PublicationNAS:UniProtKB
Negative regulation of apoptotic processTAS:Reactome
Negative regulation of glial cell apoptotic processManual Assertion Based On ExperimentIMP:UniProtKB
Negative regulation of neuron apoptotic processManual Assertion Based On ExperimentIDA:UniProtKB
Peptidyl-serine phosphorylationManual Assertion Based On ExperimentIDA:WormBase
Positive regulation of endothelial cell apoptotic processManual Assertion Based On ExperimentIMP:UniProtKB
Positive regulation of glial cell proliferationManual Assertion Based On ExperimentIMP:UniProtKB
Positive regulation of glucose importISS:BHF-UCL
Positive regulation of neuron projection developmentManual Assertion Based On ExperimentIMP:UniProtKB
Positive regulation of NF-kappaB transcription factor activityManual Assertion Based On ExperimentIDA:UniProtKB
Positive regulation of Notch signaling pathwayTAS:Reactome
Positive regulation of protein localization to plasma membraneISS:BHF-UCL
Protein phosphorylationManual Assertion Based On ExperimentIDA:UniProtKB
Protein targeting to membrane1 PublicationNAS:UniProtKB
Regulation of postsynaptic membrane neurotransmitter receptor levelsIEA:Ensembl
Response to interleukin-1IEA:Ensembl
Secretion1 PublicationNAS:UniProtKB
Vesicle-mediated transportManual Assertion Based On ExperimentTAS:UniProtKB

Cytoplasm
Membrane
Endosome
Nucleus
Transported into the endosome through interaction with SQSTM1/p62. After phosphorylation by SRC, transported into the nucleus through interaction with KPNB1. Colocalizes with CDK7 in the cytoplasm and nucleus. Transported to vesicular tubular clusters (VTCs) through interaction with RAB2A.

Phosphorylation at Thr-412 in the activation loop is not mandatory for activation (By similarity).
Upon neuronal growth factor (NGF) stimulation, phosphorylated by SRC at Tyr-265, Tyr-280 and Tyr-334 (PubMed:11713277, PubMed:16452474).
Phosphorylation at Tyr-265 facilitates binding to KPNB1/importin-beta regulating entry of PRKCI into the nucleus (PubMed:11891849).
Phosphorylation on Tyr-334 is important for NF-kappa-B stimulation (PubMed:11713277).
Phosphorylated at Thr-564 during the initial phase of long term potentiation (By similarity).