Mouse Anti-CECR2 Recombinant Antibody (CBWJC-2465) (CBMAB-C3533WJ)

Name: Mouse Anti-CECR2 Recombinant Antibody (CBWJC-2465)
SKU: CBMAB-C3533WJ
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-CECR2 Recombinant Antibody (CBWJC-2465) (CBMAB-C3533WJ) in WB

Immunoblotting of CECR2 protein in Cecr2+/+ and Cecr2−/− ESC in 2i+LIF medium. ...View More

Wu, L., Zhao, G., Xu, S., Kuang, J., Ming, J., Wu, G., ... & Liu, J. (2021). The nuclear factor CECR2 promotes somatic cell reprogramming by reorganizing the chromatin structure. Journal of Biological Chemistry, 296.

Distributed under Open Access license CC BY 4.0, without modification.

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBWJC-2465

Application

WB, IP, IF, ELISA

Immunogen

Amino acids 1-182 mapping at the N-terminus of CECR2 of human.

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG1, κ

Clonality

Monoclonal Antibody

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500
ELISA	1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

< 0.1% sodium azide

Concentration

0.2 mg/ml

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

CECR2, Histone Acetyl-Lysine Reader

Introduction

This gene encodes a bromodomain-containing protein that is involved in chromatin remodeling, and may additionally play a role in DNA damage response. The encoded protein functions as part of an ATP-dependent complex that is involved in neurulation. This gene is a candidate gene for Cat Eye Syndrome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2014]

Entrez Gene ID

Human	27443
Mouse	330409
Rat	500308

UniProt ID

Human	Q9BXF3
Mouse	E9Q2Z1
Rat	F1LYI0

Alternative Names

CECR2, Histone Acetyl-Lysine Reader; Cat Eye Syndrome Chromosome Region, Candidate 2; KIAA1740;

Function

Chromatin reader component of histone-modifying complexes, such as the CERF (CECR2-containing-remodeling factor) complex and ISWI-type complex (PubMed:15640247, PubMed:26365797, PubMed:22464331).
It thereby plays a role in various processes during development: required during embryogenesis for neural tube closure and inner ear development. In adults, required for spermatogenesis, via the formation of ISWI-type chromatin complexes (By similarity).
In histone-modifying complexes, CECR2 recognizes and binds acylated histones: binds histones that are acetylated and/or butyrylated (PubMed:26365797, PubMed:22464331).
May also be involved through its interaction with LRPPRC in the integration of cytoskeletal network with vesicular trafficking, nucleocytosolic shuttling, transcription, chromosome remodeling and cytokinesis (PubMed:11827465).

Biological Process

Apoptotic DNA fragmentation Source: HGNC-UCL
ATP-dependent chromatin remodeling Source: MGI
Cochlea development Source: Ensembl
Cytoskeleton-dependent cytokinesis Source: UniProtKB
Cytoskeleton organization Source: UniProtKB
Execution phase of apoptosis Source: BHF-UCL
Inner ear receptor cell stereocilium organization Source: Ensembl
Neural fold formation Source: Ensembl
Neural tube closure Source: Ensembl
Single fertilization Source: GO_Central
Vesicle-mediated transport Source: UniProtKB

Cellular Location

CERF complex; Nucleus; Euchromatin

Wang, X., Xu, C., Wang, S., Huang, W., Liu, Y., Zhang, X., ... & Liu, F. (2021). A novel tumor suppressor CECR2 down regulation links glutamine metabolism contributes tumor growth in laryngeal squamous cell carcinoma. Clinical and Translational Oncology, 1-13.

Park, S. G., Lee, D., Seo, H. R., Lee, S. A., & Kwon, J. (2020). Cytotoxic activity of bromodomain inhibitor NVS-CECR2-1 on human cancer cells. Scientific reports, 10(1), 1-15.

Zhang, M., Liu, Z., Aoshima, K., Zhang, Y., An, Y., Aoshima, A., ... & Yan, Q. (2020). CECR2 Drives Breast Cancer Metastasis by Suppressing Macrophage Inflammatory Responses. bioRxiv.

Lucas, S. C., Atkinson, S. J., Bamborough, P., Barnett, H., Chung, C. W., Gordon, L., ... & Demont, E. H. (2020). Optimization of Potent ATAD2 and CECR2 Bromodomain Inhibitors with an Atypical Binding Mode. Journal of medicinal chemistry, 63(10), 5212-5241.

Crawford, T. D., Audia, J. E., Bellon, S., Burdick, D. J., Bommi-Reddy, A., Côté, A., ... & Cochran, A. G. (2017). GNE-886: a potent and selective inhibitor of the cat eye syndrome chromosome region candidate 2 bromodomain (CECR2). ACS medicinal chemistry letters, 8(7), 737-741.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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