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Mouse Anti-AAV9 Recombinant Antibody (V2-634029) (CBMAB-AP023LY)


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Published Data
Mouse Anti-AAV9 Recombinant Antibody (ADK9) in Neutralization
In vivo neutralization of AAV9 by anti-AAV9 monoclonal antibodies. (A to C) Neutralization profiles of anti-AAV9 monoclonal antibodies in the (A) spleen, (B) heart, and (C) liver. The anti-AAV9 antibodies tested here are ADK9, HL2368, HL2370, HL2372, and HL2374, with ADK4 (anti-AAV4) as a negative control. ADK9, HL2370, and HL2372 are neutralizing to AAV9 in the tissues tested, however; HL2374 is nonneutralizing in the spleen. HL2368 is nonneutralizing in all tissues tested. ...View More
Emmanuel, S. N., Smith, J. K., Hsi, J., Tseng, Y. S., Kaplan, M., Mietzsch, M., ... & Agbandje-McKenna, M. (2022). Structurally mapping antigenic epitopes of adeno-associated virus 9: development of antibody escape variants. Journal of Virology, 96(3), e01251-21.
Mouse Anti-AAV9 Recombinant Antibody (ADK9) in Dot blot
Immuno-dot blot analysis of native rAAV capsids of the panel of AAV serotypes 1-12. All rAAV subtypes were purified by AVB chromatography except for rAAV9 and rAAV11, which were purified by sucrose gradient centrifugation. About 1010, 109, and 108 gp of serotype-specific rAAVs were spotted on a nitrocellulose membrane as indicated by the triangles to the right of the panels. The membranes were incubated with a panel of mAbs as depicted to the left of the membrane. Each mAb detects specific conformational epitopes on the surface of defined AAV capsids. Polyclonal antibody VP51 detects all AAV serotypes and served as an internal loading control for 109 gp of each rAAV serotype. ...View More
Mietzsch, M., Grasse, S., Zurawski, C., Weger, S., Bennett, A., Agbandje-McKenna, M., ... & Heilbronn, R. (2014). OneBac: platform for scalable and high-titer production of adeno-associated virus serotype 1-12 vectors for gene therapy. Human gene therapy, 25(3), 212-222.
Mouse Anti-AAV9 Recombinant Antibody (ADK9) in IF
Intracellular localization and capsid assembly of VP3 of different AAV serotypes. Indirect double immunofluorescence of HeLa cells expressing VP3 in the absence or presence of AAP derived from AAV2. Total expressed capsid proteins were localized using a polyclonal VP antiserum VP51 (VPs, green). Assembled capsids (red) were detected using MAb A20 (AAV2), ADK1 (AAV1), ADK5 (AAV5), ADK8 (AAV8), or ADK9 (AAV9). ...View More
Sonntag, F., Köther, K., Schmidt, K., Weghofer, M., Raupp, C., Nieto, K., ... & Kleinschmidt, J. A. (2011). The assembly-activating protein promotes capsid assembly of different adeno-associated virus serotypes. Journal of virology, 85(23), 12686-12697.

Summary

Host Animal
Mouse
Specificity
AAV9
Clone
V2-634029
Antibody Isotype
IgA, κ
Application
Dot blot, ELISA, IF, Neutralization

Basic Information

Immunogen
Recombinant produced adeno-associated virus type 9 (AAV9), carrying GFP
Host Species
Mouse
Specificity
AAV9
Antibody Isotype
IgA, κ
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
DB1:20-1:100

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Preservative
0.09% sodium azide
Concentration
Batch dependent
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
Adeno-Associated Virus Serotype 9
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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