Mouse Anti-CD33 Recombinant Antibody (6C5/2) (CBMAB-C8126-LY)

Name: Mouse Anti-CD33 Recombinant Antibody (6C5/2)
SKU: CBMAB-C8126-LY
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-CD33 Recombinant Antibody (6C5/2) (CBMAB-C8126-LY) in WB

Peripheral surgical wounding increases the CD33 levels in the hippocampus of 18 month-old mice as compared to sham. There is no significant difference in β-actin levels between the peripheral surgical wounding mice and sham mice. ...View More

Xu, Z., Dong, Y., Wang, H., Culley, D. J., Marcantonio, E. R., Crosby, G., ... & Xie, Z. (2014). Peripheral surgical wounding and age-dependent neuroinflammation in mice. PloS one, 9(5), e96752. Distributed under Open Access license CC BY 4.0, without modification.

Mouse Anti-CD33 Recombinant Antibody (6C5/2) (CBMAB-C8126-LY) in WB

Peripheral surgical wounding does not increase the CD33 levels in the hippocampus of 9 month-old mice as compared to sham. There is no significant difference in β-actin levels between the peripheral surgical wounding mice and sham mice. ...View More

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

6C5/2

Application

WB, IP, FC

Immunogen

Recombinant CD33 of human origin.

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG1, κ

Clonality

Monoclonal Antibody

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

< 0.1% sodium azide

Concentration

0.2 mg/ml

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

CD33 Molecule

Introduction

CD33 (CD33 Molecule) is a Protein Coding gene. Diseases associated with CD33 include Gallbladder Lymphoma and Extracutaneous Mastocytoma. Among its related pathways are Hematopoietic Stem Cell Differentiation Pathways and Lineage-specific Markers and Innate Immune System. Gene Ontology (GO) annotations related to this gene include receptor activity and carbohydrate binding.
An important paralog of this gene is SIGLEC6.

Entrez Gene ID

945

UniProt ID

P20138

Function

Sialic-acid-binding immunoglobulin-like lectin (Siglec) that plays a role in mediating cell-cell interactions and in maintaining immune cells in a resting state (PubMed:10611343, PubMed:15597323, PubMed:11320212).
Preferentially recognizes and binds alpha-2,3- and more avidly alpha-2,6-linked sialic acid-bearing glycans (PubMed:7718872).
Upon engagement of ligands such as C1q or syalylated glycoproteins, two immunoreceptor tyrosine-based inhibitory motifs (ITIMs) located in CD33 cytoplasmic tail are phosphorylated by Src-like kinases such as LCK (PubMed:28325905, PubMed:10887109).
These phosphorylations provide docking sites for the recruitment and activation of protein-tyrosine phosphatases PTPN6/SHP-1 and PTPN11/SHP-2 (PubMed:10556798, PubMed:10206955, PubMed:10887109).
In turn, these phosphatases regulate downstream pathways through dephosphorylation of signaling molecules (PubMed:10206955, PubMed:10887109).
One of the repressive effect of CD33 on monocyte activation requires phosphoinositide 3-kinase/PI3K (PubMed:15597323).

Biological Process

Cell adhesion Source: GO_Central
Cell-cell adhesion Source: ARUK-UCL
Cell-cell signaling Source: ProtInc
Immune response-inhibiting signal transduction Source: UniProtKB
Negative regulation of calcium ion transport Source: ARUK-UCL
Negative regulation of cell population proliferation Source: ProtInc
Negative regulation of interleukin-1 beta production Source: ARUK-UCL
Negative regulation of interleukin-8 production Source: ARUK-UCL
Negative regulation of monocyte activation Source: ARUK-UCL
Negative regulation of tumor necrosis factor production Source: ARUK-UCL
Neutrophil degranulation Source: Reactome
Positive regulation of protein secretion Source: UniProtKB
Positive regulation of protein tyrosine phosphatase activity Source: ARUK-UCL
Regulation of immune response Source: Reactome
Signal transduction Source: ProtInc

Cellular Location

Isoform CD33M: Cell membrane
Isoform CD33m: Peroxisome. CD33m isoform does not localize to cell surfaces but instead accumulates in peroxisomes.

Topology

Extracellular: 18-259
Helical: 260-282
Cytoplasmic: 283-364

PTM

Glycosylated. Glycosylation at Asn-100 is critical for regulating ligand recognition.
Phosphorylation of Tyr-340 is involved in binding to PTPN6 and PTPN11. Phosphorylation of Tyr-358 is involved in binding to PTPN6. LCK phosphorylates Tyr-340 efficiently and Tyr-358 to a lesser extent.

Marcinek, A., Brauchle, B., Hänel, G., Lacher, S. M., Zieger, N., Kischel, R., ... & Subklewe, M. (2021). CD33 BiTE® Construct Mediated Immunological Synapse Formation and Downstream Signaling in T Cells Is Dependent on Expression of Costimulatory Molecules on Target Cells. Blood, 138(Supplement 1), 2237-2237.

Chiu, E., Felices, M., Cichocki, F., Davis, Z., Wang, H., Tuninga, K., ... & Miller, J. S. (2021). Anti-NKG2C/IL-15/anti-CD33 killer engager directs primary and iPSC-derived NKG2C+ NK cells to target myeloid leukemia. Molecular Therapy.

Molica, M., Perrone, S., Mazzone, C., Niscola, P., Cesini, L., Abruzzese, E., & de Fabritiis, P. (2021). CD33 Expression and gentuzumab ozogamicin in acute myeloid leukemia: Two sides of the same coin. Cancers, 13(13), 3214.

Rotiroti, M. C., Buracchi, C., Arcangeli, S., Galimberti, S., Valsecchi, M. G., Perriello, V. M., ... & Tettamanti, S. (2020). Targeting CD33 in chemoresistant AML patient-derived xenografts by CAR-CIK cells modified with an improved SB transposon system. Molecular Therapy, 28(9), 1974-1986.

Griciuc, A., Federico, A. N., Natasan, J., Forte, A. M., McGinty, D., Nguyen, H., ... & Maguire, C. A. (2020). Gene therapy for Alzheimer’s disease targeting CD33 reduces amyloid beta accumulation and neuroinflammation. Human molecular genetics, 29(17), 2920-2935.

Miles, L. A., Hermans, S. J., Crespi, G. A., Gooi, J. H., Doughty, L., Nero, T. L., ... & Parker, M. W. (2019). Small molecule binding to Alzheimer risk factor CD33 promotes Aβ phagocytosis. Iscience, 19, 110-118.

Subklewe, M., Stein, A., Walter, R. B., Bhatia, R., Wei, A. H., Ritchie, D., ... & Ravandi, F. (2019). Preliminary results from a phase 1 first-in-human study of AMG 673, a novel half-life extended (HLE) anti-CD33/CD3 BiTE®(bispecific T-cell engager) in patients with relapsed/refractory (R/R) acute myeloid leukemia (AML).

Westervelt, P., Roboz, G. J., Cortes, J. E., Kantarjian, H. M., Lee, S., Rettig, M. P., ... & DiPersio, J. F. (2018). Phase 1 first-in-human trial of AMV564, a bivalent bispecific (2x2) CD33/CD3 T-cell Engager, in patients with relapsed/refractory acute myeloid leukemia (AML). Blood, 132, 1455.

Son, M., Diamond, B., Volpe, B. T., Aranow, C. B., Mackay, M. C., & Santiago-Schwarz, F. (2017). Evidence for C1q-mediated crosslinking of CD33/LAIR-1 inhibitory immunoreceptors and biological control of CD33/LAIR-1 expression. Scientific reports, 7(1), 1-13.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Antibody Pairs

CD33 Matched Antibody Pair (217) (CAT#: APMAB-217LY)

CD33 Matched Antibody Pair (218) (CAT#: APMAB-218LY)

CD33 Matched Antibody Pair (219) (CAT#: APMAB-219LY)

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CD33 Matched Antibody Pair (219) (CAT#: APMAB-219LY)

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For research use only. Not intended for any clinical use.

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