RAD18

The protein encoded by this gene is highly similar to S. cerevisiae DNA damage repair protein Rad18. Yeast Rad18 functions through its interaction with Rad6, which is an ubiquitin-conjugating enzyme required for post-replication repair of damaged DNA. Similar to its yeast counterpart, this protein is able to interact with the human homolog of yeast Rad6 protein through a conserved ring-finger motif. Mutation of this motif results in defective replication of UV-damaged DNA and hypersensitivity to multiple mutagens. [provided by RefSeq, Jul 2008]

RAD18, E3 Ubiquitin Protein Ligase

E3 ubiquitin-protein ligase involved in postreplication repair of UV-damaged DNA. Postreplication repair functions in gap-filling of a daughter strand on replication of damaged DNA. Associates to the E2 ubiquitin conjugating enzyme UBE2B to form the UBE2B-RAD18 ubiquitin ligase complex involved in mono-ubiquitination of DNA-associated PCNA on 'Lys-164'. Has ssDNA binding activity.

Cellular response to DNA damage stimulusManual Assertion Based On ExperimentIDA:UniProtKB
DNA repair1 PublicationNAS:UniProtKB
Negative regulation of cell deathManual Assertion Based On ExperimentIDA:UniProtKB
Positive regulation of chromosome segregationManual Assertion Based On ExperimentIMP:UniProtKB
Postreplication repairManual Assertion Based On ExperimentIBA:GO_Central
Protein autoubiquitinationManual Assertion Based On ExperimentIDA:MGI
Protein monoubiquitinationManual Assertion Based On ExperimentIBA:GO_Central

Nucleus
Cytoplasm, cytoskeleton, microtubule organizing center, centrosome
Associates with chromatin (PubMed:25931565).
Colocalizes with SLF1 in the nucleus and to centrosomes (PubMed:15632077).
Relocalizes with SLF1 to nuclear foci in response to DNA damage (PubMed:22036607).
Accumulates with the SLF1-SLF2 and SMC5-SMC6 complexes at replication-coupled DNA interstrand repair and DNA double-strand breaks (DSBs) sites on chromatin in a ubiquitin-dependent manner (PubMed:25931565).