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Mouse Anti-RAD18 Antibody (11BA3) (CBMAB-1531CQ)

This product is a mouse antibody that recognizes RAD18. The antibody 11BA3 can be used for immunoassay techniques such as: ELISA.
See all RAD18 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
11BA3
Antibody Isotype
IgG1
Application
ELISA

Basic Information

Immunogen
Synthetic Peptide (RNHLLQFALEp(S)PAKS)
Specificity
Human
Antibody Isotype
IgG1
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Supernatant
Purity
>95% as determined by analysis by SDS-PAGE
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
RAD18, E3 Ubiquitin Protein Ligase
Introduction
The protein encoded by this gene is highly similar to S. cerevisiae DNA damage repair protein Rad18. Yeast Rad18 functions through its interaction with Rad6, which is an ubiquitin-conjugating enzyme required for post-replication repair of damaged DNA. Diseases associated with RAD18 include Fanconi Anemia, Complementation Group A. Among its related pathways are DNA Damage and Translesion synthesis by Y family DNA polymerases bypasses lesions on DNA template.
Entrez Gene ID
56852
UniProt ID
Q9NS91
Alternative Names
RAD18 Homolog (S. Cerevisiae); Postreplication Repair Protein RAD18; RNF73; hHR18; hRAD18; RING Finger Protein 73; E3 Ubiquitin-Protein Ligase RAD18; Postreplication Repair Protein HRAD18p; RAD18; S. Cerevisiae; Homolog; EC 6.3.2.
Function
E3 ubiquitin-protein ligase involved in postreplication repair of UV-damaged DNA. Postreplication repair functions in gap-filling of a daughter strand on replication of damaged DNA. Associates to the E2 ubiquitin conjugating enzyme UBE2B to form the UBE2B-RAD18 ubiquitin ligase complex involved in mono-ubiquitination of DNA-associated PCNA on 'Lys-164'. Has ssDNA binding activity.
Biological Process
Cellular response to DNA damage stimulusManual Assertion Based On ExperimentIDA:UniProtKB
DNA repair1 PublicationNAS:UniProtKB
Negative regulation of cell deathManual Assertion Based On ExperimentIDA:UniProtKB
Positive regulation of chromosome segregationManual Assertion Based On ExperimentIMP:UniProtKB
Postreplication repairManual Assertion Based On ExperimentIBA:GO_Central
Protein autoubiquitinationManual Assertion Based On ExperimentIDA:MGI
Protein monoubiquitinationManual Assertion Based On ExperimentIBA:GO_Central
Cellular Location
Nucleus
Cytoplasm, cytoskeleton, microtubule organizing center, centrosome
Associates with chromatin (PubMed:25931565).
Colocalizes with SLF1 in the nucleus and to centrosomes (PubMed:15632077).
Relocalizes with SLF1 to nuclear foci in response to DNA damage (PubMed:22036607).
Accumulates with the SLF1-SLF2 and SMC5-SMC6 complexes at replication-coupled DNA interstrand repair and DNA double-strand breaks (DSBs) sites on chromatin in a ubiquitin-dependent manner (PubMed:25931565).
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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