Mouse Anti-AQP2 Recombinant Antibody (G-3) (CBMAB-A3359-YC)

Name: Mouse Anti-AQP2 Recombinant Antibody (G-3)
SKU: CBMAB-A3359-YC
Rating: 5 (1 reviews)

See all AQP2 antibodies

Compare Online Inquiry

Published Data

Mouse Anti-AQP2 Recombinant Antibody (G-3) in IF and IHC

βγ-CAT in AQP regulation.
a Colocalization of βγ-CAT and BmAQP2 in the UB tissue of toad B. maxima after toads were placed in isotonic, hypertonic or hypertonic/isotonic Ringer's solution for 3 hours as analyzed by immunohistofluorescence. Scale bars, 25 μm. b Intracellular colocalization of βγ-CAT and AQP2 in MDCK cells with or without the treatment of 10 nM βγ-CAT for 15 minutes as determined by immunofluorescence. Scale bars, 30 μm. All data are representative of at least two independent experiments. ...View More

Zhao, Z., Shi, Z. H., Ye, C. J., & Zhang, Y. (2022). A pore-forming protein drives macropinocytosis to facilitate toad water maintaining. Communications Biology, 5(1), 730.

Mouse Anti-AQP2 Recombinant Antibody (G-3) in IF

Effect of CQ administration on renal AQP2 expression. Kidney sections from rats after 4 weeks of treatment with vehicle or CQ were stained with anti-AQP2 antibody. ...View More

Wang, B., Guo, H., Ling, L., Ji, J., Niu, J., & Gu, Y. (2020). The chronic adverse effect of chloroquine on kidney in rats through an autophagy dependent and independent pathways. Nephron, 144(2), 96-108.

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

G-3

Application

WB, IP, IF, ELISA, IHC-P

Immunogen

Amino acids 232-271 within a C-terminal cytoplasmic domain of AQP2 of human origin.

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500
IHC-P	1:50-1:500
ELISA	1:30-1:3,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

< 0.1% sodium azide

Concentration

0.2 mg/ml

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Aquaporin 2

Introduction

AQP2 is a water channel protein located in the kidney collecting tubule. It belongs to the MIP/aquaporin family, some members of which are clustered together on chromosome 12q13. Mutations in this gene have been linked to autosomal dominant and recessive

Entrez Gene ID

359

UniProt ID

P41181

Alternative Names

Aquaporin 2; Water Channel Protein For Renal Collecting Duct; Collecting Duct Water Channel Protein; Aquaporin 2 (Collecting Duct); ADH Water Channel; Aquaporin-CD;

Function

Forms a water-specific channel that provides the plasma membranes of renal collecting duct with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient (PubMed:8140421, PubMed:7524315, PubMed:7510718, PubMed:15509592). Plays an essential role in renal water homeostasis (PubMed:8140421, PubMed:7524315, PubMed:15509592).

Biological Process

Cellular response to copper ion Source: UniProtKB
Cellular response to mercury ion Source: UniProtKB
Cellular response to water deprivation Source: Ensembl
Glycerol transport Source: UniProtKB
Metanephric collecting duct development Source: Ensembl
Protein homotetramerization Source: UniProtKB
Renal water homeostasis Source: UniProtKB
Renal water transport Source: Ensembl
Water transport Source: UniProtKB

Cellular Location

Apical cell membrane; Basolateral cell membrane; Cell membrane; Trans-Golgi network membrane; Cytoplasmic vesicle membrane. Shuttles from vesicles to the apical membrane (PubMed:15509592). Vasopressin-regulated phosphorylation is required for translocation to the apical cell membrane (PubMed:15509592). PLEKHA8/FAPP2 is required to transport AQP2 from the TGN to sites where AQP2 is phosphorylated (By similarity).

Involvement in disease

Diabetes insipidus, nephrogenic, autosomal (ANDI): A disorder caused by the inability of the renal collecting ducts to absorb water in response to arginine vasopressin. Characterized by excessive water drinking (polydipsia), excessive urine excretion (polyuria), persistent hypotonic urine, and hypokalemia. Inheritance can be autosomal dominant or recessive.

Topology

Cytoplasmic: 1-11 aa
Helical: 12-32 aa
Extracellular: 33-40 aa
Helical: 41-59 aa
Cytoplasmic: 60-64 aa
Discontinuously helical: 65-74 aa
Cytoplasmic: 75-85 aa
Helical: 86-107 aa
Extracellular: 108-127 aa
Helical: 128-148 aa
Cytoplasmic: 149-156 aa
Helical: 157-176 aa
Extracellular: 177-180 aa
Discontinuously helical: 181-193 aa
Extracellular: 194-201 aa
Helical: 202-222 aa
Cytoplasmic: 223-271 aa

PTM

Ser-256 phosphorylation is necessary and sufficient for expression at the apical membrane. Endocytosis is not phosphorylation-dependent.
N-glycosylated.

Ranieri, M., Di Mise, A., Centrone, M., D’Agostino, M., Tingskov, S. J., Venneri, M., ... & Tamma, G. (2021). Olive Leaf Extract (OLE) impaired vasopressin-induced aquaporin-2 trafficking through the activation of the calcium-sensing receptor. Scientific reports, 11(1), 1-13.

Kim, S., Jo, C. H., & Kim, G. H. (2021). Psychotropic drugs upregulate aquaporin-2 via vasopressin-2 receptor/cAMP/protein kinase A signaling in inner medullary collecting duct cells. American Journal of Physiology-Renal Physiology, 320(5), F963-F971.

Fenton, R. A., Murali, S. K., & Moeller, H. B. (2020). Advances in Aquaporin-2 trafficking mechanisms and their implications for treatment of water balance disorders. American Journal of Physiology-Cell Physiology, 319(1), C1-C10.

Ozer, E. S., Moeller, H. B., Karaduman, T., Fenton, R. A., & Mergen, H. (2020). Molecular characterization of an aquaporin-2 mutation causing a severe form of nephrogenic diabetes insipidus. Cellular and Molecular Life Sciences, 77(5), 953-962.

Ranieri, M., Di Mise, A., Tamma, G., & Valenti, G. (2019). Vasopressin–aquaporin-2 pathway: recent advances in understanding water balance disorders. F1000Research, 8.

Jung, H. J., & Kwon, T. H. (2019). New insights into the transcriptional regulation of aquaporin-2 and the treatment of X-linked hereditary nephrogenic diabetes insipidus. Kidney research and clinical practice, 38(2), 145.

Bräsen, J. H., Mederacke, Y. S., Schmitz, J., Diahovets, K., Khalifa, A., Hartleben, B., ... & Mederacke, I. (2019). Cholemic nephropathy causes acute kidney injury and is accompanied by loss of aquaporin 2 in collecting ducts. Hepatology, 69(5), 2107-2119.

Wu, Q., Moeller, H. B., Stevens, D. A., Sanchez-Hodge, R., Childers, G., Kortenoeven, M. L., ... & Fenton, R. A. (2018). CHIP regulates aquaporin-2 quality control and body water homeostasis. Journal of the American Society of Nephrology, 29(3), 936-948.

Roche, J. V., Survery, S., Kreida, S., Nesverova, V., Ampah-Korsah, H., Gourdon, M., ... & Törnroth-Horsefield, S. (2017). Phosphorylation of human aquaporin 2 (AQP2) allosterically controls its interaction with the lysosomal trafficking protein LIP5. Journal of Biological Chemistry, 292(35), 14636-14648.

Yui, N., Ando, F., Sasaki, S., & Uchida, S. (2017). Ser-261 phospho-regulation is involved in pS256 and pS269-mediated aquaporin-2 apical translocation. Biochemical and biophysical research communications, 490(3), 1039-1044.

Ask a question We look forward to hearing from you.

0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Have you used Mouse Anti-AQP2 Recombinant Antibody (G-3)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon

$30 eGift Card
Submit a review

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Related Products

Mouse Anti-AQP2 Recombinant Antibody (CBT3587) (CAT#: V2LY-0625-LY796)

Mouse Anti-AQP2 Recombinant Antibody (CBT2528) (CAT#: V2LY-0625-LY795)

Mouse Anti-AQP2 Recombinant Antibody (CBYC-A723) (CAT#: CBMAB-A3360-YC)

Mouse Anti-AQP2 Recombinant Antibody (E-2) (CAT#: CBMAB-A3358-YC)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Request bulk or custom quote Second antibody and isotype control

Contact us

Tel: (USA)
(UK)
Fax:

Global Locations

Email:

Online Inquiry