Mouse Anti-AQP2 Recombinant Antibody (CBT2528) (V2LY-0625-LY795)

Name: Mouse Anti-AQP2 Recombinant Antibody (CBT2528)
SKU: V2LY-0625-LY795
Rating: 5 (1 reviews)

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Tested Data

Mouse Anti-AQP2 Recombinant Antibody (CBT2528) (V2LY-0625-LY795) in ELISA

Mouse Anti-AQP2 Recombinant Antibody (CBT2528) (V2LY-0625-LY795) in WB

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBT2528

Application

Immunogen

Purified recombinant fragment of human AQP2 (AA: 149-271) expressed in E. Coli.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG1

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:500-1:2,000
ELISA	1:10,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS

Preservative

Sodium azide

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Aquaporin 2

Entrez Gene ID

359

UniProt ID

P41181

Function

Forms a water-specific channel that provides the plasma membranes of renal collecting duct with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient (PubMed:8140421, PubMed:7524315, PubMed:7510718, PubMed:15509592). Plays an essential role in renal water homeostasis (PubMed:8140421, PubMed:7524315, PubMed:15509592).

Biological Process

Cellular response to copper ion Source: UniProtKB
Cellular response to mercury ion Source: UniProtKB
Cellular response to water deprivation Source: Ensembl
Glycerol transport Source: UniProtKB
Metanephric collecting duct development Source: Ensembl
Protein homotetramerization Source: UniProtKB
Renal water homeostasis Source: UniProtKB
Renal water transport Source: Ensembl
Water transport Source: UniProtKB

Cellular Location

Apical cell membrane; Basolateral cell membrane; Cell membrane; Trans-Golgi network membrane; Cytoplasmic vesicle membrane. Shuttles from vesicles to the apical membrane (PubMed:15509592). Vasopressin-regulated phosphorylation is required for translocation to the apical cell membrane (PubMed:15509592). PLEKHA8/FAPP2 is required to transport AQP2 from the TGN to sites where AQP2 is phosphorylated (By similarity).

Involvement in disease

Diabetes insipidus, nephrogenic, autosomal (ANDI): A disorder caused by the inability of the renal collecting ducts to absorb water in response to arginine vasopressin. Characterized by excessive water drinking (polydipsia), excessive urine excretion (polyuria), persistent hypotonic urine, and hypokalemia. Inheritance can be autosomal dominant or recessive.

Topology

Cytoplasmic: 1-11 aa
Helical: 12-32 aa
Extracellular: 33-40 aa
Helical: 41-59 aa
Cytoplasmic: 60-64 aa
Discontinuously helical: 65-74 aa
Cytoplasmic: 75-85 aa
Helical: 86-107 aa
Extracellular: 108-127 aa
Helical: 128-148 aa
Cytoplasmic: 149-156 aa
Helical: 157-176 aa
Extracellular: 177-180 aa
Discontinuously helical: 181-193 aa
Extracellular: 194-201 aa
Helical: 202-222 aa
Cytoplasmic: 223-271 aa

PTM

Ser-256 phosphorylation is necessary and sufficient for expression at the apical membrane. Endocytosis is not phosphorylation-dependent.
N-glycosylated.

Ranieri, M., Di Mise, A., Centrone, M., D’Agostino, M., Tingskov, S. J., Venneri, M., ... & Tamma, G. (2021). Olive Leaf Extract (OLE) impaired vasopressin-induced aquaporin-2 trafficking through the activation of the calcium-sensing receptor. Scientific reports, 11(1), 1-13.

Kim, S., Jo, C. H., & Kim, G. H. (2021). Psychotropic drugs upregulate aquaporin-2 via vasopressin-2 receptor/cAMP/protein kinase A signaling in inner medullary collecting duct cells. American Journal of Physiology-Renal Physiology, 320(5), F963-F971.

Fenton, R. A., Murali, S. K., & Moeller, H. B. (2020). Advances in Aquaporin-2 trafficking mechanisms and their implications for treatment of water balance disorders. American Journal of Physiology-Cell Physiology, 319(1), C1-C10.

Ozer, E. S., Moeller, H. B., Karaduman, T., Fenton, R. A., & Mergen, H. (2020). Molecular characterization of an aquaporin-2 mutation causing a severe form of nephrogenic diabetes insipidus. Cellular and Molecular Life Sciences, 77(5), 953-962.

Ranieri, M., Di Mise, A., Tamma, G., & Valenti, G. (2019). Vasopressin–aquaporin-2 pathway: recent advances in understanding water balance disorders. F1000Research, 8.

Jung, H. J., & Kwon, T. H. (2019). New insights into the transcriptional regulation of aquaporin-2 and the treatment of X-linked hereditary nephrogenic diabetes insipidus. Kidney research and clinical practice, 38(2), 145.

Bräsen, J. H., Mederacke, Y. S., Schmitz, J., Diahovets, K., Khalifa, A., Hartleben, B., ... & Mederacke, I. (2019). Cholemic nephropathy causes acute kidney injury and is accompanied by loss of aquaporin 2 in collecting ducts. Hepatology, 69(5), 2107-2119.

Wu, Q., Moeller, H. B., Stevens, D. A., Sanchez-Hodge, R., Childers, G., Kortenoeven, M. L., ... & Fenton, R. A. (2018). CHIP regulates aquaporin-2 quality control and body water homeostasis. Journal of the American Society of Nephrology, 29(3), 936-948.

Roche, J. V., Survery, S., Kreida, S., Nesverova, V., Ampah-Korsah, H., Gourdon, M., ... & Törnroth-Horsefield, S. (2017). Phosphorylation of human aquaporin 2 (AQP2) allosterically controls its interaction with the lysosomal trafficking protein LIP5. Journal of Biological Chemistry, 292(35), 14636-14648.

Yui, N., Ando, F., Sasaki, S., & Uchida, S. (2017). Ser-261 phospho-regulation is involved in pS256 and pS269-mediated aquaporin-2 apical translocation. Biochemical and biophysical research communications, 490(3), 1039-1044.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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