RNF34

The protein encoded by this gene contains a RINF finger, a motif known to be involved in protein-protein and protein-DNA interactions. This protein interacts with DNAJA3/hTid-1, which is a DnaJ protein reported to function as a modulator of apoptosis. Overexpression of this gene in Hela cells was shown to confer the resistance to TNF-alpha induced apoptosis, suggesting an anti-apoptotic function of this protein. This protein can be cleaved by caspase-3 during the induction of apoptosis. This protein also targets p53 and phospho-p53 for degradation. Alternatively splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Feb 2012]

Full Name

Ring Finger Protein 34

Function

E3 ubiquitin-protein ligase that regulates several biological processes through the ubiquitin-mediated proteasomal degradation of various target proteins. Ubiquitinates the caspases CASP8 and CASP10, promoting their proteasomal degradation, to negatively regulate cell death downstream of death domain receptors in the extrinsic pathway of apoptosis (PubMed:15069192).
May mediate 'Lys-48'-linked polyubiquitination of RIPK1 and its subsequent proteasomal degradation thereby indirectly regulating the tumor necrosis factor-mediated signaling pathway (Ref.13). Negatively regulates p53/TP53 through its direct ubiquitination and targeting to proteasomal degradation (PubMed:17121812).
Indirectly, may also negatively regulate p53/TP53 through ubiquitination and degradation of SFN (PubMed:18382127).
Mediates PPARGC1A proteasomal degradation probably through ubiquitination thereby indirectly regulating the metabolism of brown fat cells (PubMed:22064484).
Possibly involved in innate immunity, through 'Lys-48'-linked polyubiquitination of NOD1 and its subsequent proteasomal degradation (PubMed:25012219).

Biological Process

Biological Process apoptotic processIEA:UniProtKB-KW
Biological Process cellular response to coldIEA:Ensembl
Biological Process negative regulation of cysteine-type endopeptidase activity involved in execution phase of apoptosisManual Assertion Based On ExperimentIMP:UniProtKB
Biological Process negative regulation of extrinsic apoptotic signaling pathway via death domain receptorsManual Assertion Based On ExperimentIMP:UniProtKB
Biological Process negative regulation of signal transduction by p53 class mediatorManual Assertion Based On ExperimentIMP:UniProtKB
Biological Process nucleotide-binding domain, leucine rich repeat containing receptor signaling pathwayManual Assertion Based On ExperimentIMP:UniProtKB
Biological Process proteasome-mediated ubiquitin-dependent protein catabolic processManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process protein K48-linked ubiquitinationManual Assertion Based On ExperimentIMP:UniProtKB
Biological Process protein ubiquitinationManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process regulation of oxygen metabolic processISS:UniProtKB
Biological Process regulation of signal transduction by p53 class mediatorTAS:Reactome
Biological Process ubiquitin-dependent protein catabolic processManual Assertion Based On ExperimentIMP:UniProtKB

Cellular Location

Cell membrane
Endomembrane system
Nucleus
Nucleus speckle
Cytoplasm, cytosol

PTM

Autoubiquitinated (in vitro).
Proteolytically cleaved by caspases upon induction of apoptosis by TNF.