USP5

Ubiquitin (see MIM 191339)-dependent proteolysis is a complex pathway of protein metabolism implicated in such diverse cellular functions as maintenance of chromatin structure, receptor function, and degradation of abnormal proteins. A late step of the process involves disassembly of the polyubiquitin chains on degraded proteins into ubiquitin monomers. USP5 disassembles branched polyubiquitin chains by a sequential exo mechanism, starting at the proximal end of the chain (Wilkinson et al., 1995 [PubMed 7578059]).[supplied by OMIM, Mar 2010]

Full Name

Ubiquitin Specific Peptidase 5

Function

Cleaves linear and branched multiubiquitin polymers with a marked preference for branched polymers. Involved in unanchored 'Lys-48'-linked polyubiquitin disassembly. Binds linear and 'Lys-63'-linked polyubiquitin with a lower affinity. Knock-down of USP5 causes the accumulation of p53/TP53 and an increase in p53/TP53 transcriptional activity because the unanchored polyubiquitin that accumulates is able to compete with ubiquitinated p53/TP53 but not with MDM2 for proteasomal recognition.

Biological Process

Biological Process positive regulation of proteasomal ubiquitin-dependent protein catabolic process Source:UniProtKB1 Publication
Biological Process protein deubiquitination Source:ParkinsonsUK-UCL1 Publication
Biological Process protein K48-linked deubiquitination Source:UniProtKB1 Publication
Biological Process protein ubiquitination Source:Reactome
Biological Process ubiquitin-dependent protein catabolic process Source:InterPro

Cellular Location

cytosol
lysosome
nucleus

Anti-USP5 antibodies

Mouse Anti-USP5 Recombinant Antibody (CBFYU-260) (CBMAB-U0260-FY)

Datasheet

SDS

Target: USP5

Host: Mouse

Specificity: Human

Clone: CBFYU-260

Application*: WB, IH, E

Mouse Anti-USP5 Recombinant Antibody (CBXU-103) (CBMAB-U0199-CQ)

Datasheet

SDS

Target: USP5

Host: Mouse

Antibody Isotype: IgG1

Specificity: Human

Clone: CBXU-103

Application*: E, WB

Mouse Anti-USP5 Recombinant Antibody (CBXU-048) (CBMAB-U0163-CQ)

Datasheet

SDS

Target: USP5

Host: Mouse

Antibody Isotype: IgG1, κ

Specificity: Human

Clone: CBXU-048

Application*: WB

Rabbit Anti-USP5 Recombinant Antibody (CBXU-019) (CBMAB-U0135-CQ)

Datasheet

SDS

Target: USP5

Host: Rabbit

Antibody Isotype: IgG

Specificity: Human, Mouse, Rat

Clone: CBXU-019

Application*: WB, F, IC

Rabbit Anti-USP5 Recombinant Antibody (CBXU-018) (CBMAB-U0134-CQ)

Datasheet

SDS

Target: USP5

Host: Rabbit

Antibody Isotype: IgG

Specificity: Human, Mouse, Rat

Clone: CBXU-018

Application*: WB, IP

Mouse Anti-USP5 (aa 71-180) Recombinant Antibody (4G4) (CBMAB-U0086-CQ)

Datasheet

SDS

Target: USP5

Host: Mouse

Antibody Isotype: IgG2a, κ

Specificity: Human

Clone: 4G4

Application*: E, WB

Mouse Anti-USP5 Recombinant Antibody (2H5) (CBMAB-U0059-CQ)

Datasheet

SDS

Target: USP5

Host: Mouse

Antibody Isotype: IgG2a

Specificity: Human, Dog, Monkey, Rat

Clone: 2H5

Application*: IH, P, WB

Mouse Anti-USP5 Recombinant Antibody (2F5) (CBMAB-U0057-CQ)

Datasheet

SDS

Target: USP5

Host: Mouse

Antibody Isotype: IgG1

Specificity: Human

Clone: 2F5

Application*: IC, IH, P, WB

Mouse Anti-USP5 Recombinant Antibody (2E3) (CBMAB-U0053-CQ)

Datasheet

SDS

Target: USP5

Host: Mouse

Antibody Isotype: IgG2a

Specificity: Human, Dog, Monkey, Rat

Clone: 2E3

Application*: IH, P, WB

Mouse Anti-USP5 (aa 71-181) Recombinant Antibody (2C8) (CBMAB-U0049-CQ)

Datasheet

SDS

Target: USP5

Host: Mouse

Antibody Isotype: IgG2a, κ

Specificity: Human

Clone: 2C8

Application*: E, WB

Mouse Anti-USP5 Recombinant Antibody (1F9) (CBMAB-U0035-CQ)

Datasheet

SDS

Target: USP5

Host: Mouse

Antibody Isotype: IgG1

Specificity: Human

Clone: 1F9

Application*: WB

Mouse Anti-USP5 Recombinant Antibody (1F8) (CBMAB-U0034-CQ)

Datasheet

SDS

Target: USP5

Host: Mouse

Antibody Isotype: IgG2a

Specificity: Human, Dog, Monkey, Rat

Clone: 1F8

Application*: IH, P, WB

Mouse Anti-USP5 Recombinant Antibody (1A8) (CBMAB-U0016-CQ)

Datasheet

SDS

Target: USP5

Host: Mouse

Antibody Isotype: IgG1

Specificity: Human

Clone: 1A8

Application*: IH, P, WB

Mouse Anti-USP5 Recombinant Antibody (CF468) (CBMAB-FT051LY)

Datasheet

SDS

Target: USP5

Host: Mouse

Antibody Isotype: IgG1

Specificity: Human

Clone: CF468

Application*: ELISA, WB

For Research Use Only. Not For Clinical Use.

(P): Predicted

* Abbreviations

IFImmunofluorescence

IHImmunohistochemistry

IPImmunoprecipitation

WBWestern Blot

EELISA

MMicroarray

CIChromatin Immunoprecipitation

FFlow Cytometry

FNFunction Assay

IDImmunodiffusion

RRadioimmunoassay

TCTissue Culture

GSGel Supershift

NNeutralization

BBlocking

AActivation

IInhibition

DDepletion

ESELISpot

DBDot Blot

MCMass Cytometry/CyTOF

CTCytotoxicity

SStimulation

AGAgonist

APApoptosis

IMImmunomicroscopy

BABioassay

CSCostimulation

EMElectron Microscopy

IEImmunoelectrophoresis

PAPeptide Array

ICImmunocytochemistry

PEPeptide ELISA

MDMeDIP

SHIn situ hybridization

IAEnzyme Immunoassay

SEsandwich ELISA

PLProximity Ligation Assay

ECELISA(Cap)

EDELISA(Det)

BIBioimaging

IOImmunoassay

LFLateral Flow Immunoassay

LALuminex Assay

CImmunohistochemistry-Frozen Sections

PImmunohistologyp-Paraffin Sections

ISIntracellular Staining for Flow Cytometry

MSElectrophoretic Mobility Shift Assay

RIRNA Binding Protein Immunoprecipitation (RIP)