ZDHHC3

ZDHHC3 (Zinc Finger DHHC-Type Containing 3) is a Protein Coding gene. Gene Ontology (GO) annotations related to this gene include protein-cysteine S-palmitoyltransferase activity and palmitoyltransferase activity. An important paralog of this gene is ZDHHC7.Palmitoyltransferase with broad specificity (PubMed:21926431, PubMed:23034182). Palmitoylates GABA receptors on their gamma subunit (GABRG1, GABRG2 and GABRG3), which regulates synaptic clustering and/or cell surface stability (By similarity). Palmitoylates glutamate receptors GRIA1 and GRIA2, which leads to their retention in Golgi. May also palmitoylate DLG4, DNAJC5 and SNAP25 (By similarity).

Full Name

Zinc Finger DHHC-Type Containing 3

Function

Golgi-localized palmitoyltransferase that catalyzes the addition of palmitate onto various protein substrates (PubMed:19001095, PubMed:21926431, PubMed:22240897, PubMed:23034182, PubMed:22314500).
Has no stringent fatty acid selectivity and in addition to palmitate can also transfer onto target proteins myristate from tetradecanoyl-CoA and stearate from octadecanoyl-CoA (By similarity).
Plays an important role in G protein-coupled receptor signaling pathways involving GNAQ and potentially other heterotrimeric G proteins by regulating their dynamic association with the plasma membrane (PubMed:19001095).
Palmitoylates ITGA6 and ITGB4, thereby regulating the alpha-6/beta-4 integrin localization, expression and function in cell adhesion to laminin (PubMed:22314500).
Plays a role in the TRAIL-activated apoptotic signaling pathway most probably through the palmitoylation and localization to the plasma membrane of TNFRSF10A (PubMed:22240897).
In the brain, by palmitoylating the gamma subunit GABRG2 of GABA(A) receptors and regulating their postsynaptic accumulation, plays a role in synaptic GABAergic inhibitory function and GABAergic innervation. Palmitoylates the neuronal protein GAP43 which is also involved in the formation of GABAergic synapses. Palmitoylates NCDN thereby regulating its association with endosome membranes. Probably palmitoylates PRCD and is involved in its proper localization within the photoreceptor. Could mediate the palmitoylation of NCAM1 and regulate neurite outgrowth. Could palmitoylate DNAJC5 and regulate its localization to Golgi membranes. Also constitutively palmitoylates DLG4. May also palmitoylate SNAP25. Could palmitoylate the glutamate receptors GRIA1 and GRIA2 but this has not been confirmed in vivo (By similarity).
Could also palmitoylate the D2 dopamine receptor DRD2 (PubMed:26535572).
May also function as a calcium transporter.

Biological Process

Biological Process lipoprotein localization to membrane Source:UniProtKB1 Publication
Biological Process peptidyl-L-cysteine S-palmitoylation Source:UniProtKB
Biological Process positive regulation of receptor localization to synapse Source:UniProtKB
Biological Process positive regulation of synaptic transmission, GABAergic Source:UniProtKB
Biological Process protein localization to photoreceptor outer segment Source:MGI1 Publication
Biological Process protein localization to plasma membrane Source:UniProtKB1 Publication
Biological Process protein palmitoylation Source:UniProtKB2 Publications
Biological Process protein targeting to membrane Source:GO_Central1 Publication
Biological Process regulation of G protein-coupled receptor signaling pathway Source:UniProtKB1 Publication
Biological Process TRAIL-activated apoptotic signaling pathway Source:UniProtKB1 Publication

Cellular Location

Golgi apparatus membrane
Localizes to the Golgi cis cisterna.

Topology

Cytoplasmic: 1-47
Helical: 48-68
Extracellular: 69-72
Helical: 73-93
Cytoplasmic: 94-171
Helical: 172-192
Extracellular: 193-214
Helical: 215-235
Cytoplasmic: 236-299

PTM

Autopalmitoylated.
Phosphorylation by FGFR1 and SRC probably regulates the palmitoyltransferase activity.