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Rabbit Anti-ADRA1A Recombinant Antibody (V2-12532) (CBMAB-1022-CN)

This product is a rabbit antibody that recognizes ADRA1A of human. The antibody EPR9691(B) can be used for immunoassay techniques such as: WB, IF, FC.
See all ADRA1A antibodies
Published Data
Rabbit Anti-ADRA1A Recombinant Antibody (EPR9691(B)) (CBMAB-1022-CN) in Immunoblot
Mice with a genetic deletion for GPR55 are characterised by reduced cardiac expression of α1A-AR. α1A-AR mRNA (A) and α1A-AR protein (B) expression in cardiac tissue from both WT and GPR55-/- mice. Quantified values are presented as the mean ± SEM. *P < 0.05 vs. WT. n = 4-5. ...View More
Walsh, S. K., Lipina, C., Ang, S. Y., Sato, M., Chia, L. Y., Kocan, M., ... & Wainwright, C. L. (2021). GPR55 regulates the responsiveness to, but does not dimerise with, α1A-adrenoceptors. Biochemical Pharmacology, 188, 114560.
Rabbit Anti-ADRA1A Recombinant Antibody (EPR9691(B)) (CBMAB-1022-CN) in FC
Quantification of receptor expression levels by flow cytometry in HTLA cells transfected with CXCR4-Tango alone (blue line) or with CXCR4-Tango plus α1a-AR, AVPR1A and ACKR3 (orange lines). Grey areas: unstained cells. ...View More
Gao, X., Enten, G. A., DeSantis, A. J., & Majetschak, M. (2021). Class AG protein-coupled receptors assemble into functional higher-order hetero-oligomers. FEBS letters, 595(14), 1863-1875.
Rabbit Anti-ADRA1A Recombinant Antibody (EPR9691(B)) (CBMAB-1022-CN) in PLA
Detection of heteromers between α1A/B/D-ARs, CXCR4, and ACKR3 upon depletion of AVPR1A in hVSMCs.
Representative PLA images for the detection of receptor-receptor interactions in hVSMC after incubation with non-targeting (NT) siRNA or AVPR1A siRNA. Images show merged PLA/4',6-diamidino-2-phenylindole dihydrochloride (DAPI) signals. Scale bar = 10 μm. ...View More
Albee, L. J., Gao, X., & Majetschak, M. (2021). Plasticity of seven-transmembrane-helix receptor heteromers in human vascular smooth muscle cells. Plos one, 16(6), e0253821.

Summary

Host Animal
Rabbit
Specificity
Human, Mouse, Rat
Clone
V2-12532
Antibody Isotype
IgG
Application
WB, IF, IHC-F

Basic Information

Immunogen
Synthetic peptide within Human alpha 1a Adrenergic Receptor/ADRA1A aa 200-300.
Host Species
Rabbit
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:1,000-1:10,000
IF(ICC)1:250-1:500
FC1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
40% Glycerol, 0.05% BSA
Preservative
0.1% sodium azide
Concentration
Batch dependent
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Adrenoceptor Alpha 1A
Introduction
Alpha-1-adrenergic receptors (alpha-1-ARs) are members of the G protein-coupled receptor superfamily. They activate mitogenic responses and regulate growth and proliferation of many cells. There are 3 alpha-1-AR subtypes: alpha-1A, -1B and -1D, all of which signal through the Gq/11 family of G-proteins and different subtypes show different patterns of activation. This gene encodes alpha-1A-adrenergic receptor. Alternative splicing of this gene generates four transcript variants, which encode four different isoforms with distinct C-termini but having similar ligand binding properties. This alpha-adrenergic receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. The effect is mediated by G(q) and G(11) proteins. Nuclear ADRA1A-ADRA1B heterooligomers regulate phenylephrine(PE)-stimulated ERK signaling in cardiac myocytes.
Entrez Gene ID
Human148
Mouse11549
Rat29412
UniProt ID
HumanP35348
MouseP97718
RatP43140
Alternative Names
ADRA1C; ADRA1L1; ALPHA1AAR
Function
This alpha-adrenergic receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. Its effect is mediated by G(q) and G(11) proteins. Nuclear ADRA1A-ADRA1B heterooligomers regulate phenylephrine(PE)-stimulated ERK signaling in cardiac myocytes.
Biological Process
Activation of phospholipase C activity
Adenylate cyclase-activating adrenergic receptor signaling pathway
Adult heart development
Aging
Apoptotic process
Calcium ion transport into cytosol
Cell-cell signaling
Cell growth involved in cardiac muscle cell development
G protein-coupled receptor signaling pathway
Intracellular signal transduction
Micturition
Negative regulation of autophagy
Negative regulation of cell population proliferation
Negative regulation of heart rate involved in baroreceptor response to increased systemic arterial blood pressure
Negative regulation of Rho protein signal transduction
Neuron-glial cell signaling
Norepinephrine-epinephrine vasoconstriction involved in regulation of systemic arterial blood pressure
Phospholipase C-activating G protein-coupled receptor signaling pathway
Pilomotor reflex
Positive regulation of action potential
Positive regulation of cardiac muscle contraction
Positive regulation of cardiac muscle hypertrophy
Positive regulation of cytosolic calcium ion concentration
Positive regulation of ERK1 and ERK2 cascade
Positive regulation of heart rate by epinephrine-norepinephrine
Positive regulation of MAPK cascade
Positive regulation of non-membrane spanning protein tyrosine kinase activity
Positive regulation of protein kinase C signaling
Positive regulation of smooth muscle contraction
Positive regulation of synaptic transmission, GABAergic
Positive regulation of systemic arterial blood pressure
Positive regulation of the force of heart contraction by epinephrine-norepinephrine
Positive regulation of vasoconstriction
Regulation of synaptic vesicle exocytosis
Response to drug
Response to hormone
Signal transduction
Smooth muscle contraction
Cellular Location
Cytoplasm; Cell membrane; Nucleus membrane; Caveola. Location at the nuclear membrane facilitates heterooligomerization and regulates ERK-mediated signaling in cardiac myocytes. Colocalizes with GNAQ, PLCB1 as well as LAP2 at the nuclear membrane of cardiac myocytes.
Topology
Extracellular: 1-27 aa
Helical: 28-51 aa
Cytoplasmic: 52-64 aa
Helical: 65-88 aa
Extracellular: 89-99 aa
Helical: 100-122 aa
Cytoplasmic: 123-143 aa
Helical: 144-167 aa
Extracellular: 168-181 aa
Helical: 182-205 aa
Cytoplasmic: 206-273 aa
Helical: 274-297 aa
Extracellular: 298-305 aa
Helical: 306-329 aa
Cytoplasmic: 330-466 aa
PTM
C-terminal Ser or Thr residues may be phosphorylated.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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