Mouse Anti-ADRA1A Recombinant Antibody (V2-180017) (CBMAB-A1410-YC)

Provided herein is a Mouse monoclonal antibody against Human Adrenoceptor Alpha 1A. The antibody can be used for immunoassay techniques, such as ELISA, WB.
See all ADRA1A antibodies

Datasheet

Summary

Host Animal

Mouse

Specificity

Human

Clone

V2-180017

Antibody Isotype

IgG1, κ

Application

ELISA, WB

Basic Information

Immunogen

ADRA1A (NP_150646, 1 a.a. ~ 27 a.a) partial recombinant protein with GST tag.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG1, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

PBS, pH 7.2

Buffer

PBS, pH7.4

Preservative

None

Concentration

Batch dependent

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name

Adrenoceptor Alpha 1A

Introduction

Alpha-1-adrenergic receptors (alpha-1-ARs) are members of the G protein-coupled receptor superfamily. They activate mitogenic responses and regulate growth and proliferation of many cells. There are 3 alpha-1-AR subtypes: alpha-1A, -1B and -1D, all of whi

Entrez Gene ID

148

UniProt ID

P35348

Alternative Names

Adrenoceptor Alpha 1A; Adrenergic, Alpha-1A-, Receptor; Alpha-1C Adrenergic Receptor; Alpha-1A Adrenoreceptor; Alpha-1A Adrenoceptor; ADRA1C;

Function

This alpha-adrenergic receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. Its effect is mediated by G(q) and G(11) proteins. Nuclear ADRA1A-ADRA1B heterooligomers regulate phenylephrine(PE)-stimulated ERK signaling in cardiac myocytes.

Biological Process

Activation of phospholipase C activity
Adenylate cyclase-activating adrenergic receptor signaling pathway
Adult heart development
Aging
Apoptotic process
Calcium ion transport into cytosol
Cell-cell signaling
Cell growth involved in cardiac muscle cell development
G protein-coupled receptor signaling pathway
Intracellular signal transduction
Micturition
Negative regulation of autophagy
Negative regulation of cell population proliferation
Negative regulation of heart rate involved in baroreceptor response to increased systemic arterial blood pressure
Negative regulation of Rho protein signal transduction
Neuron-glial cell signaling
Norepinephrine-epinephrine vasoconstriction involved in regulation of systemic arterial blood pressure
Phospholipase C-activating G protein-coupled receptor signaling pathway
Pilomotor reflex
Positive regulation of action potential
Positive regulation of cardiac muscle contraction
Positive regulation of cardiac muscle hypertrophy
Positive regulation of cytosolic calcium ion concentration
Positive regulation of ERK1 and ERK2 cascade
Positive regulation of heart rate by epinephrine-norepinephrine
Positive regulation of MAPK cascade
Positive regulation of non-membrane spanning protein tyrosine kinase activity
Positive regulation of protein kinase C signaling
Positive regulation of smooth muscle contraction
Positive regulation of synaptic transmission, GABAergic
Positive regulation of systemic arterial blood pressure
Positive regulation of the force of heart contraction by epinephrine-norepinephrine
Positive regulation of vasoconstriction
Regulation of synaptic vesicle exocytosis
Response to drug
Response to hormone
Signal transduction
Smooth muscle contraction

Cellular Location

Cytoplasm; Cell membrane; Nucleus membrane; Caveola. Location at the nuclear membrane facilitates heterooligomerization and regulates ERK-mediated signaling in cardiac myocytes. Colocalizes with GNAQ, PLCB1 as well as LAP2 at the nuclear membrane of cardiac myocytes.

Topology

Extracellular: 1-27 aa
Helical: 28-51 aa
Cytoplasmic: 52-64 aa
Helical: 65-88 aa
Extracellular: 89-99 aa
Helical: 100-122 aa
Cytoplasmic: 123-143 aa
Helical: 144-167 aa
Extracellular: 168-181 aa
Helical: 182-205 aa
Cytoplasmic: 206-273 aa
Helical: 274-297 aa
Extracellular: 298-305 aa
Helical: 306-329 aa
Cytoplasmic: 330-466 aa

PTM

C-terminal Ser or Thr residues may be phosphorylated.

References

Deji, C., Li, Y., Chen, Y., Lai, J., & Wei, S. (2021). Association study of Catechol-o-methyltransferase and Alpha-1-adrenergic receptor gene polymorphisms with multiple phenotypes of heroin use disorder. Neuroscience Letters, 748, 135677.

Chen, G., Fan, X., Li, Y., He, L., Wang, S., Dai, Y., ... & Lin, H. (2020). Promoter aberrant methylation status of ADRA1A is associated with hepatocellular carcinoma. Epigenetics, 15(6-7), 684-701.

Pillay, Y., Nagiah, S., Phulukdaree, A., Krishnan, A., & Chuturgoon, A. A. (2020). Patulin suppresses α 1-adrenergic receptor expression in HEK293 cells. Scientific reports, 10(1), 1-14.

Wang, T., Qin, Y., Lai, H., Wei, W., Li, Z., Yang, Y., ... & Chen, J. (2019). The prognostic value of ADRA1 subfamily genes in gastric carcinoma. Oncology letters, 18(3), 3150-3158.

Peng, L., Peng, W., Hu, P., & Zhang, H. F. (2018). Clinical significance of expression levels of serum ADRA1A in hysterocarcinoma patients. Oncology letters, 15(6), 9162-9166.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Associated Products

Related Products

Rabbit Anti-ADRA1A Recombinant Antibody (V2-12532)

Isotype control

For research use only. Not intended for any clinical use.

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We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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