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Rabbit Anti-DLK1 Recombinant Antibody (9D8) (CBMAB-D1061-YC)


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Published Data
Rabbit Anti-DLK1 Recombinant Antibody (9D8) (CBMAB-D1061-YC-1) in FC
Rabbit Anti-DLK1 Recombinant Antibody (9D8) (CBMAB-D1061-YC-1) in FC
Flow cytometry analysis of human fetal liver cell cultures after DLK1 knockdown. Total human fetal liver cells cultured with DLK1-targeting siRNA (grey bars) or nontargeting control siRNA (black bars). Cells were analyzed for expression of hematopoietic CD45, lineage (Lin) surface antigens, CD34, CD31, and Lin-CD34+CD38- (hematopoietic stem cells). Data are given as means ± standard deviation from n = 3 biological repeats. ...View More
Gerlach, J. C., Thompson, R. L., Gridelli, B., & Schmelzer, E. (2019). Effects of Delta-Like Noncanonical Notch Ligand 1 Expression of Human Fetal Liver Hepatoblasts on Hematopoietic Progenitors. Stem cells international, 2019(1), 7916275.
Rabbit Anti-DLK1 Recombinant Antibody (9D8) (CBMAB-D1061-YC-2) in IF
Rabbit Anti-DLK1 Recombinant Antibody (9D8) (CBMAB-D1061-YC-2) in IF
Representative confocal images of testis sections from Oct4- GFP transgenic mice at P6. Stromal cells (asterisks) and some PTMs (arrowheads) are positive for DLK1 (red). Oct4- GFP indicated germ cells. Cell nuclei were stained with DAPI. Scale bar = 50 μm. ...View More
Suen, H. C., Rao, S., Luk, A. C. S., Zhang, R., Yang, L., Qi, H., ... & Liao, J. (2023). The single-cell chromatin accessibility landscape in mouse perinatal testis development. Elife, 12, e75624.

Summary

Host Animal
Rabbit
Specificity
Mouse
Clone
9D8
Antibody Isotype
IgG
Application
WB, IHC, FC

Basic Information

Immunogen
Recombinant mouse Pref-1/DLK1/FA1 (Ala24-Gln305).
Host Species
Rabbit
Specificity
Mouse
Antibody Isotype
IgG
Clonality
Monoclonal Antibody
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1 µg/ml
FC0.25 µg/10^6 cells
IHC1-25 µg/ml

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Lyophilized
Buffer
Trehalose & PBS
Preservative
None
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Delta Like Non-Canonical Notch Ligand 1
Introduction
DLK1 is a transmembrane protein that contains multiple epidermal growth factor repeats that functions as a regulator of cell growth. The encoded protein is involved in the differentiation of several cell types including adipocytes. This gene is located in a region of chromosome 14 frequently showing unparental disomy, and is imprinted and expressed from the paternal allele. A single nucleotide variant in this gene is associated with child and adolescent obesity and shows polar overdominance, where heterozygotes carrying an active paternal allele express the phenotype, while mutant homozygotes are normal.
Entrez Gene ID
13386
UniProt ID
Q09163
Alternative Names
Delta Like Non-Canonical Notch Ligand 1; DLK-1; DLK; PG2; Delta-Like 1 Homolog (Drosophila); Delta-Like Homolog (Drosophila); Protein Delta Homolog 1; Preadipocyte Factor 1; Delta-Like 1 Homolog;
Function
May have a role in neuroendocrine differentiation.
Biological Process
Cell differentiation Source: Ensembl
Negative regulation of Notch signaling pathway Source: HGNC
Cellular Location
Cytoplasm; Membrane
Topology
Extracellular: 24-303
Helical: 304-327
Cytoplasmic: 328-383
PTM
N- and O-glycosylated. O-glycosylated with core 1 or possibly core 8 glycans.
More Infomation

Pittaway, J. F., Lipsos, C., Mariniello, K., & Guasti, L. (2021). The role of delta-like non-canonical Notch ligand 1 (DLK1) in cancer. Endocrine-related cancer, 28(12), R271-R287.

Mohammadabadi, M., Masoudzadeh, S. H., Khezri, A., Kalashnyk, O., Stavetska, R. V., Klopenko, N. I., ... & Tkachenko, S. V. (2021). Fennel (Foeniculum vulgare) seed powder increases Delta-Like Non-Canonical Notch Ligand 1 gene expression in testis, liver, and humeral muscle tissues of growing lambs. Heliyon, 7(12), e08542.

Pham, A., Sobrier, M. L., Giabicani, E., Le Jules Fernandes, M., Mitanchez, D., Brioude, F., & Netchine, I. (2021). Screening of patients born small for gestational age with the Silver-Russell syndrome phenotype for DLK1 variants. European Journal of Human Genetics, 29(12), 1756-1761.

Grassi, E. S., Pantazopoulou, V., & Pietras, A. (2020). Hypoxia-induced release, nuclear translocation, and signaling activity of a DLK1 intracellular fragment in glioma. Oncogene, 39(20), 4028-4044.

Traustadóttir, G. Á., Lagoni, L. V., Ankerstjerne, L. B. S., Bisgaard, H. C., Jensen, C. H., & Andersen, D. C. (2019). The imprinted gene Delta like non-canonical Notch ligand 1 (Dlk1) is conserved in mammals, and serves a growth modulatory role during tissue development and regeneration through Notch dependent and independent mechanisms. Cytokine & Growth Factor Reviews, 46, 17-27.

Jensen, C. H., Rydén, M., Baun, C., Hvidsten, S., Andersen, M. S., Christensen, L. L., ... & Andersen, D. C. (2019). The imprinted gene Delta like non-canonical notch ligand 1 (Dlk1) associates with obesity and triggers insulin resistance through inhibition of skeletal muscle glucose uptake. EBioMedicine, 46, 368-380.

Finn, J., Sottoriva, K., Pajcini, K. V., Kitajewski, J. K., Chen, C., Zhang, W., ... & Liu, Y. (2019). Dlk1-mediated temporal regulation of Notch signaling is required for differentiation of alveolar type II to type I cells during repair. Cell reports, 26(11), 2942-2954.

Huang, C. C., Kuo, H. M., Wu, P. C., Cheng, S. H., Chang, T. T., Chang, Y. C., ... & Tai, M. H. (2018). Soluble delta-like 1 homolog (DLK1) stimulates angiogenesis through Notch1/Akt/eNOS signaling in endothelial cells. Angiogenesis, 21(2), 299-312.

Traustadóttir, G. Á., Jensen, C. H., Ramirez, J. J. G., Beck, H. C., Sheikh, S. P., & Andersen, D. C. (2017). The non-canonical NOTCH1 ligand Delta-like 1 homolog (DLK1) self interacts in mammals. International journal of biological macromolecules, 97, 460-467.

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For research use only. Not intended for any clinical use.

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