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Mouse Anti-DLK1 Recombinant Antibody (CBYC-P055) (V2LY-0425-LY472)

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Published Data

Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat
Clone
CBYC-P055
Antibody Isotype
IgG3, κ
Application
WB, IP, IF, ELISA, IHC-P

Basic Information

Immunogen
Amino acids 266-383 of human DLK1.
Host Species
Mouse
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG3, κ
Clonality
Monoclonal Antibody
Application Notes
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)1:50-1:500
ELISA1:100-1:1,000
IHC-P1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
Gelatin & PBS
Preservative
Sodium Azide
Concentration
0.2 mg/ml
Purity
>95% as determined by analysis by SDS-PAGE
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
Delta Like Non-Canonical Notch Ligand 1
Entrez Gene ID
Human8788
Mouse13386
UniProt ID
HumanP80370
MouseQ09163
Function
May have a role in neuroendocrine differentiation.
Biological Process
Cell differentiation Source: Ensembl
Negative regulation of Notch signaling pathway Source: HGNC
Cellular Location
Cytoplasm; Membrane
Topology
Extracellular: 24-303
Helical: 304-327
Cytoplasmic: 328-383
PTM
N- and O-glycosylated. O-glycosylated with core 1 or possibly core 8 glycans.
More Infomation

Pittaway, J. F., Lipsos, C., Mariniello, K., & Guasti, L. (2021). The role of delta-like non-canonical Notch ligand 1 (DLK1) in cancer. Endocrine-related cancer, 28(12), R271-R287.

Mohammadabadi, M., Masoudzadeh, S. H., Khezri, A., Kalashnyk, O., Stavetska, R. V., Klopenko, N. I., ... & Tkachenko, S. V. (2021). Fennel (Foeniculum vulgare) seed powder increases Delta-Like Non-Canonical Notch Ligand 1 gene expression in testis, liver, and humeral muscle tissues of growing lambs. Heliyon, 7(12), e08542.

Pham, A., Sobrier, M. L., Giabicani, E., Le Jules Fernandes, M., Mitanchez, D., Brioude, F., & Netchine, I. (2021). Screening of patients born small for gestational age with the Silver-Russell syndrome phenotype for DLK1 variants. European Journal of Human Genetics, 29(12), 1756-1761.

Grassi, E. S., Pantazopoulou, V., & Pietras, A. (2020). Hypoxia-induced release, nuclear translocation, and signaling activity of a DLK1 intracellular fragment in glioma. Oncogene, 39(20), 4028-4044.

Traustadóttir, G. Á., Lagoni, L. V., Ankerstjerne, L. B. S., Bisgaard, H. C., Jensen, C. H., & Andersen, D. C. (2019). The imprinted gene Delta like non-canonical Notch ligand 1 (Dlk1) is conserved in mammals, and serves a growth modulatory role during tissue development and regeneration through Notch dependent and independent mechanisms. Cytokine & Growth Factor Reviews, 46, 17-27.

Jensen, C. H., Rydén, M., Baun, C., Hvidsten, S., Andersen, M. S., Christensen, L. L., ... & Andersen, D. C. (2019). The imprinted gene Delta like non-canonical notch ligand 1 (Dlk1) associates with obesity and triggers insulin resistance through inhibition of skeletal muscle glucose uptake. EBioMedicine, 46, 368-380.

Finn, J., Sottoriva, K., Pajcini, K. V., Kitajewski, J. K., Chen, C., Zhang, W., ... & Liu, Y. (2019). Dlk1-mediated temporal regulation of Notch signaling is required for differentiation of alveolar type II to type I cells during repair. Cell reports, 26(11), 2942-2954.

Huang, C. C., Kuo, H. M., Wu, P. C., Cheng, S. H., Chang, T. T., Chang, Y. C., ... & Tai, M. H. (2018). Soluble delta-like 1 homolog (DLK1) stimulates angiogenesis through Notch1/Akt/eNOS signaling in endothelial cells. Angiogenesis, 21(2), 299-312.

Traustadóttir, G. Á., Jensen, C. H., Ramirez, J. J. G., Beck, H. C., Sheikh, S. P., & Andersen, D. C. (2017). The non-canonical NOTCH1 ligand Delta-like 1 homolog (DLK1) self interacts in mammals. International journal of biological macromolecules, 97, 460-467.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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