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Mouse Anti-DLK1 Recombinant Antibody (CBYC-P055) (V2LY-0425-LY472)


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Published Data
Mouse Anti-DLK1 Recombinant Antibody (CBYC-P055) (V2LY-0425-LY472-1) in IHC
Mouse Anti-DLK1 Recombinant Antibody (CBYC-P055) (V2LY-0425-LY472-1) in IHC
Tissue sections of the liver from BYM/GREB1 high (C4) and BYM/GREB1 low (C3) were stained with indicated antibodies and hematoxylin. ...View More
Matsumoto, S., Yamamichi, T., Shinzawa, K., Kasahara, Y., Nojima, S., Kodama, T., ... & Kikuchi, A. (2019). GREB1 induced by Wnt signaling promotes development of hepatoblastoma by suppressing TGFβ signaling. Nature communications, 10(1), 3882.
Mouse Anti-DLK1 Recombinant Antibody (CBYC-P055) (V2LY-0425-LY472-2) in WB
Mouse Anti-DLK1 Recombinant Antibody (CBYC-P055) (V2LY-0425-LY472-2) in WB
Representative western blot of CDKN1C and DLK1 in primary pancreatic islet cells treated with or without 30 mM glucose (HG) for 48 h. ...View More
Yang, T., Yan, C., Yang, L., Tan, J., Jiang, S., Hu, J., ... & Li, Y. (2023). Identification and validation of core genes for type 2 diabetes mellitus by integrated analysis of single-cell and bulk RNA-sequencing. European Journal of Medical Research, 28(1), 340.
Mouse Anti-DLK1 Recombinant Antibody (CBYC-P055) (V2LY-0425-LY472-3) in WB
Mouse Anti-DLK1 Recombinant Antibody (CBYC-P055) (V2LY-0425-LY472-3) in WB
Protein expression related to adipogenesis and lipolysis in the control and Tg(+) groups at differentiation 0 and 8 days. ...View More
Son, D., & Lee, M. (2023). Gene regulation of RMR-related DNAJC6 on adipogenesis and mitochondria function in 3T3-L1 preadipocytes. Biochemical and Biophysical Research Communications, 672, 1-9.
Mouse Anti-DLK1 Recombinant Antibody (CBYC-P055) (V2LY-0425-LY472-4) in WB
Mouse Anti-DLK1 Recombinant Antibody (CBYC-P055) (V2LY-0425-LY472-4) in WB
Western blot result validating that Dlk1 was almost completely knocked out after poly I:C inducement. The LSK were lysed for protein loading. ...View More
Huang, D., Han, Y., Tang, T., Yang, L., Jiang, P., Qian, W., ... & Qian, P. (2023). Dlk1 maintains adult mice long-term HSCs by activating Notch signaling to restrict mitochondrial metabolism. Experimental Hematology & Oncology, 12(1), 11.
Mouse Anti-DLK1 Recombinant Antibody (CBYC-P055) (V2LY-0425-LY472-5) in IF
Mouse Anti-DLK1 Recombinant Antibody (CBYC-P055) (V2LY-0425-LY472-5) in IF
Characterization of mouse sympathetic neuroblasts. Dissociated E14.5 sympathetic ganglion cells were plated on porn/laminin-coated dishes in serum-free culture medium and analyzed after cell attachment. Double-immunostaining showed that all PHOX2B-positive cells co-express the noradrenergic markers TH (A) and VMAT1 (B) but not the chromaffin cell-specific proteins PNMT (C), DLK1 (D), and SGII (E). ISLET1 (F), TUBB3 (G), and PERIPHERIN (H) expression are shown as examples of neuron-specific proteins. Magnification bar, 60 μm. ...View More
Sriha, J., Louis-Brennetot, C., Pierre-Eugène, C., Baulande, S., Raynal, V., Kramdi, A., ... & Rohrer, H. (2022). BET and CDK inhibition reveal differences in the proliferation control of sympathetic ganglion neuroblasts and adrenal chromaffin cells. Cancers, 14(11), 2755.

Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat
Clone
CBYC-P055
Antibody Isotype
IgG3, κ
Application
WB, IP, IF, ELISA, IHC-P

Basic Information

Immunogen
Amino acids 266-383 of human DLK1.
Host Species
Mouse
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG3, κ
Clonality
Monoclonal Antibody
Application Notes
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)1:50-1:500
ELISA1:100-1:1,000
IHC-P1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
Gelatin & PBS
Preservative
Sodium Azide
Concentration
0.2 mg/ml
Purity
>95% as determined by analysis by SDS-PAGE
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
Delta Like Non-Canonical Notch Ligand 1
Entrez Gene ID
Human8788
Mouse13386
UniProt ID
HumanP80370
MouseQ09163
Function
May have a role in neuroendocrine differentiation.
Biological Process
Cell differentiation Source: Ensembl
Negative regulation of Notch signaling pathway Source: HGNC
Cellular Location
Cytoplasm; Membrane
Topology
Extracellular: 24-303
Helical: 304-327
Cytoplasmic: 328-383
PTM
N- and O-glycosylated. O-glycosylated with core 1 or possibly core 8 glycans.
More Infomation

Pittaway, J. F., Lipsos, C., Mariniello, K., & Guasti, L. (2021). The role of delta-like non-canonical Notch ligand 1 (DLK1) in cancer. Endocrine-related cancer, 28(12), R271-R287.

Mohammadabadi, M., Masoudzadeh, S. H., Khezri, A., Kalashnyk, O., Stavetska, R. V., Klopenko, N. I., ... & Tkachenko, S. V. (2021). Fennel (Foeniculum vulgare) seed powder increases Delta-Like Non-Canonical Notch Ligand 1 gene expression in testis, liver, and humeral muscle tissues of growing lambs. Heliyon, 7(12), e08542.

Pham, A., Sobrier, M. L., Giabicani, E., Le Jules Fernandes, M., Mitanchez, D., Brioude, F., & Netchine, I. (2021). Screening of patients born small for gestational age with the Silver-Russell syndrome phenotype for DLK1 variants. European Journal of Human Genetics, 29(12), 1756-1761.

Grassi, E. S., Pantazopoulou, V., & Pietras, A. (2020). Hypoxia-induced release, nuclear translocation, and signaling activity of a DLK1 intracellular fragment in glioma. Oncogene, 39(20), 4028-4044.

Traustadóttir, G. Á., Lagoni, L. V., Ankerstjerne, L. B. S., Bisgaard, H. C., Jensen, C. H., & Andersen, D. C. (2019). The imprinted gene Delta like non-canonical Notch ligand 1 (Dlk1) is conserved in mammals, and serves a growth modulatory role during tissue development and regeneration through Notch dependent and independent mechanisms. Cytokine & Growth Factor Reviews, 46, 17-27.

Jensen, C. H., Rydén, M., Baun, C., Hvidsten, S., Andersen, M. S., Christensen, L. L., ... & Andersen, D. C. (2019). The imprinted gene Delta like non-canonical notch ligand 1 (Dlk1) associates with obesity and triggers insulin resistance through inhibition of skeletal muscle glucose uptake. EBioMedicine, 46, 368-380.

Finn, J., Sottoriva, K., Pajcini, K. V., Kitajewski, J. K., Chen, C., Zhang, W., ... & Liu, Y. (2019). Dlk1-mediated temporal regulation of Notch signaling is required for differentiation of alveolar type II to type I cells during repair. Cell reports, 26(11), 2942-2954.

Huang, C. C., Kuo, H. M., Wu, P. C., Cheng, S. H., Chang, T. T., Chang, Y. C., ... & Tai, M. H. (2018). Soluble delta-like 1 homolog (DLK1) stimulates angiogenesis through Notch1/Akt/eNOS signaling in endothelial cells. Angiogenesis, 21(2), 299-312.

Traustadóttir, G. Á., Jensen, C. H., Ramirez, J. J. G., Beck, H. C., Sheikh, S. P., & Andersen, D. C. (2017). The non-canonical NOTCH1 ligand Delta-like 1 homolog (DLK1) self interacts in mammals. International journal of biological macromolecules, 97, 460-467.

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For research use only. Not intended for any clinical use.

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