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Creative Biolabs has successfully developed SARS, MERS and other anti-viral antibodies in the past to assist scientific research. Facing the difficult COVID-19, Creative Biolabs actively invests in antibody development to assist researchers to better understand the characteristics of 2019-nCoV and drug development. Currently, Creative Biolabs is proud to offer an extensive line of research antibodies to support the study of SARS-CoV-2/COVID-19, several of which were validated using virus-infected cell lysates.

Explore the collections of SARS-CoV-2/COVID-19 antibodies >

Porcine T Cell Response Antibody Array (Membrane, 19 Targets) (Y0577) (AbAr-0577-YC)

Antibody array is a specific form of protein microarray. In this technology, capture antibodies spotted on membranes or glass slide bind to specific target proteins present in the sample. Captured proteins are visualized using chemiluminescent or Laser Scanner. The signal produced is proportional to the amount of analyte bound. The product provided herein is a Porcine T Cell Response Antibody Array, which can be used for semi-quantitative detection of 19 porcine T cell response factors. It is suitable for a range of liquid forms including Cell culture supernatants, Cell lysates, Plasma, Serum and Tissue lysates.
Specifications
Target Array
T Cell Response Array
Target
GM-CSF, IFN-gamma, IL-1 alpha(IL-1 F1), IL-1 beta(IL-1 F2), IL-1 Ra(IL-1 F3), IL-10, IL-12 p70, IL-13, IL-15, IL-17A, IL-17F, IL-21 IL-22, IL-28B, IL-4, IL-6, NCAM-1(CD56), TGF beta 1, TNF alpha
Species Reactivity
Porcine
Sample Type
Cell culture supernatants, Cell lysates, Plasma, Serum and Tissue lysates
Applications
Application
Multiplexed Protein Detection
Assay Type
Semi-Quantitative
Solid Support
Membrane
Detection Assay
Sandwich-based Assay
Protocol Outline
Block the array membrane surface
Incubate with Sample
Incubate with Biotinylated Detection Antibody Cocktail
Incubate with HRP Conjugated Streptavidin
Incubate with Detection Buffers
Image with chemiluminescent imaging system
Analyze data
Components
Antibody Array Membrane
Labeling Reagent
Blocking Buffer
Washing Buffer
Detection Buffer
Detection Cocktail
Others
Storage
Store at -20°C for 6 months. Once thawed, please keep reagents under suitable conditions respectively for no more than 3 months. Do not use past expiration date.
Background
Introduction
Cell-mediated immune responses involve the destruction of infected cells by cytotoxic T cells, or the destruction of intracellular pathogens by macrophages. The activation of naive T cells in response to antigen, and their subsequent proliferation and differentiation, constitutes a primary immune response.
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