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Porcine T Cell Response Antibody Array (Membrane, 19 Targets) (Y0577) (AbAr-0577-YC)

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Specifications

Target Array
T Cell Response Array
Target
GM-CSF, IFNG, IL-1 alpha, IL-1 beta, IL-1 Ra, IL-10, IL-12 p70, IL-13, IL-15, IL-17A, IL-17F, IL-21 IL-22, IL-28B, IL-4, IL-6, NCAM-1, TGF beta 1, TNF alpha
Species Reactivity
Porcine
Sample Type
Cell culture supernatants, Cell lysates, Plasma, Serum and Tissue lysates

Applications

Application
Multiplexed Protein Detection
Assay Type
Semi-Quantitative
Solid Support
Membrane
Detection Assay
Sandwich-based Assay
Protocol Outline
Conduct blocking of the array membrane surface.
Proceed with incubation of the sample.
Incubate with a biotinylated detection antibody cocktail.
Continue with incubation using HRP-streptavidin.
Engage in incubation with detection buffers.
Capture images using a chemiluminescent imaging system.
Perform data analysis.
Components
Antibody Array Membrane
Labeling Reagent
Blocking Buffer
Washing Buffer
Detection Buffer
Detection Cocktail
Others
Storage
Store at -20°C for 6 months. Once thawed, please keep reagents under suitable conditions respectively for no more than 3 months. Do not use past expiration date.

Background

Introduction
Cell-mediated immune responses involve the destruction of infected cells by cytotoxic T cells, or the destruction of intracellular pathogens by macrophages. The activation of naive T cells in response to antigen, and their subsequent proliferation and differentiation, constitutes a primary immune response.
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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Alternative Versions

Human T Cell Response Antibody Array (Membrane, 10 Targets) (Y0573) (CAT#: AbAr-0573-YC)

Human T Cell Response Antibody Array (Membrane, 20 Targets) (Y0574) (CAT#: AbAr-0574-YC)

Canine T Cell Response Antibody Array (Membrane, 13 Targets) (Y0575) (CAT#: AbAr-0575-YC)

Bovine T Cell Response Antibody Array (Membrane, 10 Targets) (Y0576) (CAT#: AbAr-0576-YC)

Antibody Array

Human T Cell Response Antibody Array (Membrane, 10 Targets) (Y0573) (CAT#: AbAr-0573-YC)

Human T Cell Response Antibody Array (Membrane, 20 Targets) (Y0574) (CAT#: AbAr-0574-YC)

Canine T Cell Response Antibody Array (Membrane, 13 Targets) (Y0575) (CAT#: AbAr-0575-YC)

Bovine T Cell Response Antibody Array (Membrane, 10 Targets) (Y0576) (CAT#: AbAr-0576-YC)

Related Products

Canine T Cell Response Antibody Array (Membrane, 13 Targets) (Y0575) (CAT#: AbAr-0575-YC)

Human T Cell Response Antibody Array (Membrane, 10 Targets) (Y0573) (CAT#: AbAr-0573-YC)

Human T Cell Response Antibody Array (Membrane, 20 Targets) (Y0574) (CAT#: AbAr-0574-YC)

Bovine T Cell Response Antibody Array (Membrane, 10 Targets) (Y0576) (CAT#: AbAr-0576-YC)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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