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Rat Anti-A4GALT Recombinant Antibody (V2-178843) (CBMAB-A0059-YC)

Provided herein is a Rat monoclonal antibody against Human Alpha 1,4-Galactosyltransferase (P Blood Group). The antibody can be used for immunoassay techniques, such as FC, IHC-Fr.
See all A4GALT antibodies

Summary

Host Animal
Rat
Specificity
Human, Cattle
Clone
V2-178843
Antibody Isotype
IgM
Application
FC, IHC-Fr

Basic Information

Immunogen
Daudi cell line
Specificity
Human, Cattle
Antibody Isotype
IgM
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
FC1 µg/ml

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
PBS, 0.2% BSA, 0.1% sodium azide
Buffer
TBS, pH8.0
Preservative
15mM sodium azide
Concentration
1 mg/ml
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Alpha 1,4-Galactosyltransferase (P Blood Group)
Introduction
A4GALT catalyzes the transfer of galactose to lactosylceramide to form globotriaosylceramide, which has been identified as the P(k) antigen of the P blood group system. This protein, a type II membrane protein found in the Golgi, is also required for the
Entrez Gene ID
Human53947
Cattle618369
UniProt ID
HumanQ9NPC4
CattleG3MZ03
Alternative Names
Alpha 1,4-Galactosyltransferase (P Blood Group); Globotriaosylceramide Synthase; CD77 Synthase; UDP-Galactose:Beta-D-Galactosyl-Beta1-R 4-Alpha-D-Galactosyltransferase; Lactosylceramide 4-Alpha-Galactosyltransferase; Alpha-1,4-N-Acetylglucosaminyltransfer
Function
Catalyzes the transfer of galactose from UDP-alpha-D-galactose to lactosylceramide/beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide(d18:1(4E)) to produce globotriaosylceramide/globoside Gb3Cer (d18:1(4E)). Also able to transfer galactose to galactosylceramide/beta-D-Gal-(1<->1')-Cer. Globoside Gb3Cer is a glycosphingolipid of the globo serie, one of the major types of neutral root structures of glycosphingolipids, that constitute a significant portion of mammalian cell membranes (Probable). Globotriaosylceramide/globoside Gb3Cer in blood and tissue cell membranes is the antigen Pk of blood histogroup P.
Biological Process
Globoside biosynthetic process
Glycosphingolipid biosynthetic process
Plasma membrane organization
Cellular Location
Golgi apparatus membrane; Membrane protein
Topology
Cytoplasmic: 1-22 aa
Helical: 23-43 aa
Lumenal: 44-353 aa

Westman, J. S., Stenfelt, L., Vidovic, K., Möller, M., Hellberg, Å., Kjellström, S., & Olsson, M. L. (2018). Allele-selective RUNX1 binding regulates P1 blood group status by transcriptional control of A4GALT. Blood, The Journal of the American Society of Hematology, 131(14), 1611-1616.

Yeh, C. C., Chang, C. J., Twu, Y. C., Hung, S. T., Tsai, Y. J., Liao, J. C., ... & Yu, L. C. (2018). The differential expression of the blood group P1‐A4GALT and P2‐A4GALT alleles is stimulated by the transcription factor early growth response 1. Transfusion, 58(4), 1054-1064.

Kaczmarek, R., Szymczak-Kulus, K., Bereźnicka, A., Mikołajczyk, K., Duk, M., Majorczyk, E., ... & Czerwinski, M. (2018). Single nucleotide polymorphisms in A4GALT spur extra products of the human Gb3/CD77 synthase and underlie the P1PK blood group system. PloS one, 13(4), e0196627.

Maruyama, H., Taguchi, A., Nishikawa, Y., Guili, C., Mikame, M., Nameta, M., ... & Ishii, S. (2018). Medullary thick ascending limb impairment in the GlatmTg (CAG‐A4GALT) Fabry model mice. The FASEB Journal, 32(8), 4544-4559.

Shastry, S., Satyamoorthy, K., Acharya, K. V., Reddy, V. R., Mohan, G., Deepika, C., ... & Joshi, M. B. (2020). Deletion in the A4GALT gene associated with Rare “P null” phenotype: The first report from India. Transfusion Medicine and Hemotherapy, 47(2), 186-189.

Thinley, J., Nathalang, O., Chidtrakoon, S., & Intharanut, K. (2021). Blood group P1 prediction using multiplex PCR genotyping of A4GALT among Thai blood donors. Transfusion Medicine, 31(1), 48-54.

Maruyama, H., Taguchi, A., Mikame, M., Lu, H., Tada, N., Ishijima, M., ... & Ishii, S. (2020). Low bone mineral density due to secondary hyperparathyroidism in the GlatmTg (CAG‐A4GALT) mouse model of Fabry disease. FASEB BioAdvances, 2(6), 365-381.

Chen, Q., Xiao, J., Duan, Z., Shi, L., Huang, C., & Li, M. (2021). Novel A4GALT gene variation with rare p phenotype in a compound heterozygous Chinese individual. Transfusion, 61(1), E5-E6.

Jacob, F., Alam, S., Liang, C. Y., Kohler, R., Nonantz, M., Everest-Dass, A., ... & Heinzelmann-Schwarz, V. (2018). A4GALT-related glycosphingolipids play a pivotal role in the reversible transition of mesenchymal and epithelial ovarian cancer cells and hence are important regulators of metastasis. Geburtshilfe und Frauenheilkunde, 78(10), P-152.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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