Human A4GALT ELISA Kit (V2LY-0626-LY4790)

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Tested Data
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Basic Information

Sensitivity
0.0077 ng/mL
Detection Range
0.015-3 ng/mL
Sample Type
Serum, Plasma, cell culture supernates
Specificity
Human
Assay Type
Sandwich
Reactivity
Human
Assay Time
1.5 h
Molecule Mass
40.5 kDa
Components
  • Pre-coated ELISA plate: 12 wells * 8 detachable strips
  • Standard solution: 0.5ml x1
  • Standard diluent: 3ml x1
  • Streptavidin-HRP: 6ml x1
  • Stop solution: 6ml x1
  • Substrate solution A: 6ml x1
  • Substrate solution B: 6ml x1
  • Wash buffer concentrate (25x): 20ml x1
  • Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 2-8°C
More Infomation

Target

Full Name
Alpha 1,4-Galactosyltransferase (P Blood Group)
Function
Catalyzes the transfer of galactose from UDP-alpha-D-galactose to lactosylceramide/beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide(d18:1(4E)) to produce globotriaosylceramide/globoside Gb3Cer (d18:1(4E)). Also able to transfer galactose to galactosylceramide/beta-D-Gal-(1<->1')-Cer. Globoside Gb3Cer is a glycosphingolipid of the globo serie, one of the major types of neutral root structures of glycosphingolipids, that constitute a significant portion of mammalian cell membranes (Probable). Globotriaosylceramide/globoside Gb3Cer in blood and tissue cell membranes is the antigen Pk of blood histogroup P.
Biological Process
Globoside biosynthetic process
Glycosphingolipid biosynthetic process
Plasma membrane organization
Cellular Location
Golgi apparatus membrane; Membrane protein
Topology
Cytoplasmic: 1-22 aa
Helical: 23-43 aa
Lumenal: 44-353 aa

Westman, J. S., Stenfelt, L., Vidovic, K., Möller, M., Hellberg, Å., Kjellström, S., & Olsson, M. L. (2018). Allele-selective RUNX1 binding regulates P1 blood group status by transcriptional control of A4GALT. Blood, The Journal of the American Society of Hematology, 131(14), 1611-1616.

Yeh, C. C., Chang, C. J., Twu, Y. C., Hung, S. T., Tsai, Y. J., Liao, J. C., ... & Yu, L. C. (2018). The differential expression of the blood group P1‐A4GALT and P2‐A4GALT alleles is stimulated by the transcription factor early growth response 1. Transfusion, 58(4), 1054-1064.

Kaczmarek, R., Szymczak-Kulus, K., Bereźnicka, A., Mikołajczyk, K., Duk, M., Majorczyk, E., ... & Czerwinski, M. (2018). Single nucleotide polymorphisms in A4GALT spur extra products of the human Gb3/CD77 synthase and underlie the P1PK blood group system. PloS one, 13(4), e0196627.

Maruyama, H., Taguchi, A., Nishikawa, Y., Guili, C., Mikame, M., Nameta, M., ... & Ishii, S. (2018). Medullary thick ascending limb impairment in the GlatmTg (CAG‐A4GALT) Fabry model mice. The FASEB Journal, 32(8), 4544-4559.

Shastry, S., Satyamoorthy, K., Acharya, K. V., Reddy, V. R., Mohan, G., Deepika, C., ... & Joshi, M. B. (2020). Deletion in the A4GALT gene associated with Rare “P null” phenotype: The first report from India. Transfusion Medicine and Hemotherapy, 47(2), 186-189.

Thinley, J., Nathalang, O., Chidtrakoon, S., & Intharanut, K. (2021). Blood group P1 prediction using multiplex PCR genotyping of A4GALT among Thai blood donors. Transfusion Medicine, 31(1), 48-54.

Maruyama, H., Taguchi, A., Mikame, M., Lu, H., Tada, N., Ishijima, M., ... & Ishii, S. (2020). Low bone mineral density due to secondary hyperparathyroidism in the GlatmTg (CAG‐A4GALT) mouse model of Fabry disease. FASEB BioAdvances, 2(6), 365-381.

Chen, Q., Xiao, J., Duan, Z., Shi, L., Huang, C., & Li, M. (2021). Novel A4GALT gene variation with rare p phenotype in a compound heterozygous Chinese individual. Transfusion, 61(1), E5-E6.

Jacob, F., Alam, S., Liang, C. Y., Kohler, R., Nonantz, M., Everest-Dass, A., ... & Heinzelmann-Schwarz, V. (2018). A4GALT-related glycosphingolipids play a pivotal role in the reversible transition of mesenchymal and epithelial ovarian cancer cells and hence are important regulators of metastasis. Geburtshilfe und Frauenheilkunde, 78(10), P-152.

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For research use only. Not intended for any clinical use.

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