Sign in or Register   Sign in or Register
  |  

Mouse Anti-AADAC Recombinant Antibody (V2-178857) (CBMAB-A0075-YC)

Provided herein is a Mouse monoclonal antibody against Human Arylacetamide Deacetylase. The antibody can be used for immunoassay techniques, such as ELISA, WB.
See all AADAC antibodies
Published Data
Mouse Anti-AADAC Recombinant Antibody (2E8) (CBMAB-A0075-YC) in WB
SDS-PAGE and immunoblot analysis of AADAC protein expressed in PNGase F-, O-glycosidase-, and Endo H-treated HLM. HLM (30 mg) were treated with the glycosidases as described in Section 2, and subjected to electrophoresis using a 10% SDS-polyacrylamide gel. The proteins were transferred onto a PVDF membrane and the AADAC protein was detected by an anti-human AADAC antibody. ...View More
Muta, K., Fukami, T., Nakajima, M., & Yokoi, T. (2014). N-Glycosylation during translation is essential for human arylacetamide deacetylase enzyme activity. Biochemical pharmacology, 87(2), 352-359.
Mouse Anti-AADAC Recombinant Antibody (2E8) (CBMAB-A0075-YC) in WB
A, immunoblot analysis of AADAC, CES1A, and CES2 in HLM, HJM, HPM, and HRM. Total cell homogenates from COS7 cells (30 g) were separated by electrophoresis using 10% SDS-polyacrylamide gel. ...View More
Watanabe, A., Fukami, T., Nakajima, M., Takamiya, M., Aoki, Y., & Yokoi, T. (2009). Human arylacetamide deacetylase is a principal enzyme in flutamide hydrolysis. Drug metabolism and disposition, 37(7), 1513-1520.
Fig.1 The wild-type AADAC expression plasmids were transiently transfected into HuH-7 cells.
...View More
Fig.2 Immunoblot analysis of recombinant human AADAC expressed in COS7 cells.
...View More
Fig.3 Representative photograph of immunoblot analysis for determination of the expression level of recombinant intact AADAC protein.
...View More
Fig.4 immunoblot analysis of recombinant human AADAC, CES1, and CES2 expressed in COS7 cells.
...View More
Fig.5 Immunoblot analysis of AADAC, CES1A, and CES2 in HLM, HJM, HPM, and HRM.
...View More

Summary

Host Animal
Mouse
Specificity
Human
Clone
V2-178857
Antibody Isotype
IgG2b, κ
Application
ELISA, WB

Basic Information

Immunogen
AADAC (NP_001077, 201 a.a. ~ 300 a.a) partial recombinant protein with GST tag.
Specificity
Human
Antibody Isotype
IgG2b, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
PBS, pH 7.2
Buffer
PBS, pH7.4
Preservative
None
Concentration
Batch dependent
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
arylacetamide deacetylase (esterase)
Introduction
Microsomal arylacetamide deacetylase competes against the activity of cytosolic arylamine N-acetyltransferase, which catalyzes one of the initial biotransformation pathways for arylamine and heterocyclic amine carcinogens
Entrez Gene ID
13
UniProt ID
P22760
Alternative Names
Arylacetamide Deacetylase; Arylacetamide Deacetylase (Esterase); DAC; EC 3.1.1.79; EC 3.1.1.3; EC 3.1.1; CES5A1;
Function
Displays cellular triglyceride lipase activity in liver, increases the levels of intracellular fatty acids derived from the hydrolysis of newly formed triglyceride stores and plays a role in very low-density lipoprotein assembly. Displays serine esterase activity in liver. Deacetylates a variety of arylacetamide substrates, including xenobiotic compounds and procarcinogens, converting them to the primary arylamide compounds and increasing their toxicity.
Biological Process
Positive regulation of triglyceride catabolic process
Xenobiotic metabolic process
Cellular Location
Endoplasmic reticulum membrane; Microsome membrane
Topology
Cytoplasmic: 1-5 aa
Helical: 6-23 aa
Lumenal: 24-399 aa
PTM
Glycosylation is required for enzyme activity.

Hirosawa, K., Fukami, T., Tashiro, K., Sakai, Y., Kisui, F., Nakano, M., & Nakajima, M. (2021). Role of human AADAC on hydrolysis of eslicarbazepine acetate and effects of AADAC genetic polymorphisms on hydrolase activity. Drug Metabolism and Disposition.

Hirosawa, K., Fukami, T., Tashiro, K., Sakai, Y., Kisui, F., Nakano, M., & Nakajima, M. (2021). Role of Human Arylacetamide Deacetylase (AADAC) on Hydrolysis of Eslicarbazepine Acetate and Effects of AADAC Genetic Polymorphisms on Hydrolase Activity. Drug Metabolism and Disposition, 49(4), 322-329.

Honda, S., Fukami, T., Tsujiguchi, T., Zhang, Y., Nakano, M., & Nakajima, M. (2021). Hydrolase activities of cynomolgus monkey liver microsomes and recombinant CES1, CES2, and AADAC. European Journal of Pharmaceutical Sciences, 161, 105807.

Levano, K. S., Jaramillo-Valverde, L., Tarazona, D. D., Sanchez, C., Capristano, S., Solari, L., ... & Guio, H. (2021). Allelic and Genotypic Frequencies of NAT2, CYP2E1 and AADAC genes in a cohort of Peruvian Tuberculosis Patients. bioRxiv.

Misra, A., & Fisher, E. A. (2020). Translational Research in Culture: AADAC, Diabetes, and Cardiovascular Disease. Cell Stem Cell, 27(1), 6-7.

Francis, J., Zvada, S. P., Denti, P., Hatherill, M., Charalambous, S., Mungofa, S., ... & McIlleron, H. M. (2019). A population pharmacokinetic analysis shows that arylacetamide deacetylase (AADAC) gene polymorphism and HIV infection affect the exposure of rifapentine. Antimicrobial agents and chemotherapy, 63(4), e01964-18.

Francis, J., Zvada, S. P., Denti, P., Hatherill, M., Charalambous, S., Mungofa, S., ... & McIlleron, H. M. (2019). Arylacetamide deacetylase (AADAC) gene polymorphism and HIV infection affect the exposure of Rifapentine: a population pharmacokinetics analysis. Antimicrobial Agents and Chemotherapy.

Ask a question We look forward to hearing from you.
0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Loading...
Have you used Mouse Anti-AADAC Recombinant Antibody (V2-178857)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon $30 eGift Card
Submit a review
Loading...
For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Documents

Contact us

  • Tel: (USA)
  • (UK)
  • Fax:
  • Email:

Submit A Review

Go to
Compare