Mouse Anti-AADAT Recombinant Antibody (V2-12484) (CBMAB-0974-CN)

This product is a mouse antibody that recognizes AADAT of human. The antibody CBLNA-163 can be used for immunoassay techniques such as: WB, IHC, ICC, IP.
See all AADAT antibodies

Datasheet

Summary

Host Animal

Mouse

Specificity

Human

Clone

V2-12484

Antibody Isotype

IgG

Application

WB, IHC, ICC, IP

Basic Information

Specificity

Human

Antibody Isotype

IgG

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, pH 7.4, 50% Glycerol

Preservative

0.02% Sodium azide

Concentration

0.5 mg/mL

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name

Aminoadipate Aminotransferase

Introduction

This gene encodes a protein that is highly similar to mouse and rat kynurenine aminotransferase II. The rat protein is a homodimer with two transaminase activities. One activity is the transamination of alpha-aminoadipic acid, a final step in the saccaropine pathway which is the major pathway for L-lysine catabolism. The other activity involves the transamination of kynurenine to produce kynurenine acid, the precursor of kynurenic acid which has neuroprotective properties. The protein has transaminase activity towards aminoadipate, kynurenine, methionine and glutamate. It shows activity also towards tryptophan, aspartate and hydroxykynurenine.

Entrez Gene ID

51166

UniProt ID

Q8N5Z0

Alternative Names

KAT2; KATII; KYAT2

Function

Transaminase with broad substrate specificity. Has transaminase activity towards aminoadipate, kynurenine, methionine and glutamate. Shows activity also towards tryptophan, aspartate and hydroxykynurenine. Accepts a variety of oxo-acids as amino-group acceptors, with a preference for 2-oxoglutarate, 2-oxocaproic acid, phenylpyruvate and alpha-oxo-gamma-methiol butyric acid. Can also use glyoxylate as amino-group acceptor (in vitro).

Biological Process

2-oxoglutarate metabolic process
Alpha-amino acid metabolic process
Biosynthetic process
Glutamate metabolic process
Kynurenine metabolic process
L-lysine catabolic process to acetyl-CoA via saccharopine
Lysine catabolic process
Tryptophan catabolic process

Cellular Location

Mitochondrion

References

Hsu, C. C., Yang, A. Y. P., Chen, J. Y., Tsai, H. H., Lin, S. H., Tai, P. C., ... & Yang, J. C. H. (2021). Lysine Deprivation Induces AKT-AADAT Signaling and Overcomes EGFR-TKIs Resistance in EGFR-Mutant Non-Small Cell Lung Cancer Cells. Cancers, 13(2), 272.

Higazi, A. M., Kamel, H. M., Abdel-Naeem, E. A., Abdullah, N. M., Mahrous, D. M., & Osman, A. M. (2021). Expression analysis of selected genes involved in tryptophan metabolic pathways in Egyptian children with Autism Spectrum Disorder and learning disabilities. Scientific Reports, 11(1), 1-12.

Kocki, T., Wicha-Komsta, K., Zakrocka, I., Pabiś, S., & Turski, W. A. (2019). The 14-day carbamazepine treatment increases expression of KAT1 (Ccbl1) and KAT2 (Aadat) genes in rat brain cortex. European Neuropsychopharmacology, 29, S458-S459.

Teumer, A., Chaker, L., Groeneweg, S., Li, Y., Di Munno, C., Barbieri, C., ... & Medici, M. (2018). Genome-wide analyses identify a role for SLC17A4 and AADAT in thyroid hormone regulation. Nature communications, 9(1), 1-14.

Towfigh, A. (2019). Der Einfluss genetischer Polymorphismen im AADAT-Gen auf die Schizophrenie (Doctoral dissertation, lmu).

Wigner, P., Czarny, P., Synowiec, E., Bijak, M., Talarowska, M., Galecki, P., ... & Sliwinski, T. (2018). Variation of genes encoding KAT1, AADAT and IDO1 as a potential risk of depression development. European Psychiatry, 52, 95-103.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Associated Products

Related Products

Mouse Anti-AADAT Recombinant Antibody (V2-51866)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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