What applications is this ABLIM1 antibody validated for?

This Mouse Anti-ABLIM1 Recombinant Antibody (CBMAB-A0369-YC) is rigorously validated for several common laboratory applications, including Western Blot (WB), Immunofluorescence (IF)/Immunocytochemistry (ICC), and Immunoprecipitation (IP). This broad range of validated applications makes it a versatile tool for various research needs related to ABLIM1.

Can this antibody detect ABLIM1 in species other than mouse or human?

This antibody is a mouse recombinant antibody. While it is often developed and validated for human and mouse samples due to common research models and high protein sequence conservation, its reactivity with ABLIM1 from other species would depend on the degree of sequence homology within the antibody's specific epitope. Always refer to the product datasheet provided by Creative Biolabs for the most accurate and up-to-date information on validated species reactivity. If your specific species of interest is not listed, it's advisable to perform a preliminary cross-reactivity test in your own experimental system.

Mouse Anti-ABLIM1 Recombinant Antibody (V2-179126) (CBMAB-A0369-YC)

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Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

V2-179126

Application

ELISA, WB

Immunogen

Partial recombinant corresponding to aa637-736 from human ABLIM1 (NP_002304) with GST tag.

Specificity

Human

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid in PBS, pH 7.2

Buffer

PBS, pH7.4

Preservative

None

Concentration

Batch dependent

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

actin binding LIM protein 1

Introduction

ABLIM1 is a LIM zinc-binding domain-containing protein that binds to actin filaments and mediates interactions between actin and cytoplasmic targets.

Entrez Gene ID

3983

UniProt ID

O14639

Alternative Names

Actin Binding LIM Protein 1; Actin-Binding LIM Protein Family Member 1; LIMATIN; AbLIM-1; LIMAB1; ABLIM;

Function

May act as scaffold protein (By similarity). May play a role in the development of the retina. Has been suggested to play a role in axon guidance.

Biological Process

Animal organ morphogenesis
Cilium assembly
Cytoskeleton organization
Lamellipodium assembly
Visual perception

Cellular Location

Cytoskeleton; Cytoplasm. Associated with the cytoskeleton.

Choi, K., Lee, J., & Kang, H. J. (2021). Myelination defects in the medial prefrontal cortex of Fkbp5 knockout mice. The FASEB Journal, 35(2), e21297.

Chen, X., Huang, Y., Shi, D., Nie, C., Luo, Y., Guo, L., ... & Xie, C. (2020). LncRNA ZNF667-AS1 Promotes ABLIM1 Expression by Adsorbing microRNA-1290 to Suppress Nasopharyngeal Carcinoma Cell Progression. OncoTargets and therapy, 13, 4397.

Dong, X., Feng, M., Yang, H., Liu, H., Guo, H., Gao, X., ... & Kong, R. (2020). Rictor promotes cell migration and actin polymerization through regulating ABLIM1 phosphorylation in Hepatocellular Carcinoma. International journal of biological sciences, 16(15), 2835.

Narahara, H., Sakai, E., Yamaguchi, Y., Narahara, S., Iwatake, M., Okamoto, K., ... & Tsukuba, T. (2019). Actin binding LIM 1 (abLIM1) negatively controls osteoclastogenesis by regulating cell migration and fusion. Journal of cellular physiology, 234(1), 486-499.

Jin, S. H., Kim, H., Gu, D. R., Park, K. H., Lee, Y. R., Choi, Y., & Lee, S. H. (2018). Actin-binding LIM protein 1 regulates receptor activator of NF-κB ligand-mediated osteoclast differentiation and motility. BMB reports, 51(7), 356.

Li, G., Huang, S., Yang, S., Wang, J., Cao, J., Czajkowsky, D. M., ... & Zhu, X. (2018). abLIM1 constructs non-erythroid cortical actin networks to prevent mechanical tension-induced blebbing. Cell discovery, 4(1), 1-14.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Related Products

Rabbit Anti-ABLIM1 Recombinant Antibody (V2-12493) (CAT#: CBMAB-0983-CN)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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