What applications is the Mouse Anti-ACADL Recombinant Antibody (V2-179171) validated for?

This Mouse Anti-ACADL Recombinant Antibody (V2-179171) is validated for Western Blot (WB) and Immunoprecipitation (IP). While other applications might be possible, these are the ones for which Creative Biolabs provides specific validation data and recommended starting dilutions. Users are encouraged to optimize conditions for their specific experimental needs.

What species reactivity can I expect from this antibody?

The Mouse Anti-ACADL Recombinant Antibody (V2-179171) is specifically validated for reactivity with Mouse and Rat ACADL. While some antibodies may exhibit cross-reactivity with other species due to sequence homology, this is not guaranteed. If you are working with samples from a species not listed, it is advisable to contact Creative Biolabs' technical support for recommendations or to perform preliminary validation.

Mouse Anti-ACADL Recombinant Antibody (V2-179171) (CBMAB-A0428-YC)

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Published Data

Mouse Anti-ACADL Recombinant Antibody (7F5DD6) (CBMAB-A0428-YC) in WB

Representative immunoblots showing ACADL phosphorylation at Ser, Thr, and Tyr residues in cardiomyocytes stimulated or not with 8-CPT-AM (10 µM, 30 min). ...View More

Laudette, M., Sainte-Marie, Y., Cousin, G., Bergonnier, D., Belhabib, I., Brun, S., ... & Lezoualc'h, F. (2021). Cyclic AMP-binding protein Epac1 acts as a metabolic sensor to promote cardiomyocyte lipotoxicity. Cell death & disease, 12(9), 824.

Mouse Anti-ACADL Recombinant Antibody (7F5DD6) (CBMAB-A0428-YC) in WB

Expression level of fatty acid transporters (CD36 and CPT1-M), LCAD in WT. ...View More

Maurya, S. K., Herrera, J. L., Sahoo, S. K., Reis, F. C., Vega, R. B., Kelly, D. P., & Periasamy, M. (2018). Sarcolipin signaling promotes mitochondrial biogenesis and oxidative metabolism in skeletal muscle. Cell reports, 24(11), 2919-2931.

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

V2-179171

Application

WB, IP

Immunogen

Tissue, cells or virus.

Specificity

Mouse, Rat

Antibody Isotype

IgG1

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1 µg/ml

Formulations & Storage [For reference only, actual COA shall prevail!]

Buffer

HEPES, pH 7.5

Preservative

0.02% sodium azide

Concentration

Batch dependent

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Acyl-CoA Dehydrogenase Long Chain

Introduction

ACADL belongs to the acyl-CoA dehydrogenase family, which is a family of mitochondrial flavoenzymes involved in fatty acid and branched chain amino-acid metabolism. This protein is one of the four enzymes that catalyze the initial step of mitochondrial be

Entrez Gene ID

Mouse	11363
Rat	25287

UniProt ID

Mouse	P51174
Rat	P15650

Alternative Names

Acyl-CoA Dehydrogenase Long Chain; Acyl-Coenzyme A Dehydrogenase, Long Chain; LCAD; Long-Chain Specific Acyl-CoA Dehydrogenase, Mitochondrial; EC 1.3.99.13; EC 1.3.8.8; EC 1.3.99; ACAD4;

Function

Long-chain specific acyl-CoA dehydrogenase is one of the acyl-CoA dehydrogenases that catalyze the first step of mitochondrial fatty acid beta-oxidation, an aerobic process breaking down fatty acids into acetyl-CoA and allowing the production of energy from fats (By similarity). The first step of fatty acid beta-oxidation consists in the removal of one hydrogen from C-2 and C-3 of the straight-chain fatty acyl-CoA thioester, resulting in the formation of trans-2-enoyl-CoA (By similarity). Among the different mitochondrial acyl-CoA dehydrogenases, long-chain specific acyl-CoA dehydrogenase can act on saturated and unsaturated acyl-CoAs with 6 to 24 carbons with a preference for 8 to 18 carbons long primary chains.

Biological Process

Carnitine catabolic process
Carnitine metabolic process, CoA-linked
Cellular lipid catabolic process
Fatty acid beta-oxidation
Fatty acid beta-oxidation using acyl-CoA dehydrogenase
Long-chain fatty acid catabolic process
Negative regulation of fatty acid biosynthetic process
Negative regulation of fatty acid oxidation
Positive regulation of cold-induced thermogenesis
Regulation of cholesterol metabolic process
Temperature homeostasis

Cellular Location

Mitochondrion matrix

PTM

Acetylation at Lys-318 and Lys-322 in proximity of the cofactor-binding sites strongly reduces catalytic activity. These sites are deacetylated by SIRT3.

Beck, M. E., Zhang, Y., Bharathi, S. S., Kosmider, B., Bahmed, K., Dahmer, M. K., ... & Goetzman, E. S. (2020). The common K333Q polymorphism in long-chain acyl-CoA dehydrogenase (LCAD) reduces enzyme stability and function. Molecular genetics and metabolism, 131(1-2), 83-89.

Zhang, M., Sunaba, T., Sun, Y., Shibata, T., Sasaki, K., Isoda, H., ... & Kita, M. (2020). Acyl-CoA dehydrogenase long chain (ACADL) is a target protein of stylissatin A, an anti-inflammatory cyclic heptapeptide. The Journal of antibiotics, 73(8), 589-592.

Mezhnina, V., Pearce, R., Poe, A., Velingkaar, N., Astafev, A., Ebeigbe, O. P., ... & Kondratov, R. V. (2020). CR reprograms acetyl‐CoA metabolism and induces long‐chain acyl‐CoA dehydrogenase and CrAT expression. Aging cell, 19(11), e13266.

Ranea‐Robles, P., Yu, C., van Vlies, N., Vaz, F. M., & Houten, S. M. (2020). Slc22a5 haploinsufficiency does not aggravate the phenotype of the long‐chain acyl‐CoA dehydrogenase KO mouse. Journal of inherited metabolic disease, 43(3), 486-495.

Gaston, G., Gangoiti, J. A., Winn, S., Chan, B., Barshop, B. A., Harding, C. O., & Gillingham, M. B. (2020). Cardiac tissue citric acid cycle intermediates in exercised very long‐chain acyl‐CoA dehydrogenase‐deficient mice fed triheptanoin or medium‐chain triglyceride. Journal of inherited metabolic disease, 43(6), 1232-1242.

Zhang, Y., Bharathi, S. S., Beck, M. E., & Goetzman, E. S. (2019). The fatty acid oxidation enzyme long-chain acyl-CoA dehydrogenase can be a source of mitochondrial hydrogen peroxide. Redox biology, 26, 101253.

Yu, D. L., Li, H. W., Wang, Y., Li, C. Q., You, D., Jiang, L., ... & Li, X. H. (2018). Acyl-CoA dehydrogenase long chain expression is associated with esophageal squamous cell carcinoma progression and poor prognosis. OncoTargets and therapy, 11, 7643.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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