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Mouse Anti-ACADL Recombinant Antibody (V2-179171) (CBMAB-A0428-YC)

Provided herein is a Mouse monoclonal antibody against Mouse Acyl-CoA Dehydrogenase Long Chain. The antibody can be used for immunoassay techniques, such as WB, IP.
See all ACADL antibodies
Published Data
Mouse Anti-ACADL Recombinant Antibody (7F5DD6) (CBMAB-A0428-YC) in WB
Representative immunoblots showing ACADL phosphorylation at Ser, Thr, and Tyr residues in cardiomyocytes stimulated or not with 8-CPT-AM (10 µM, 30 min). ...View More
Laudette, M., Sainte-Marie, Y., Cousin, G., Bergonnier, D., Belhabib, I., Brun, S., ... & Lezoualc'h, F. (2021). Cyclic AMP-binding protein Epac1 acts as a metabolic sensor to promote cardiomyocyte lipotoxicity. Cell death & disease, 12(9), 824.
Mouse Anti-ACADL Recombinant Antibody (7F5DD6) (CBMAB-A0428-YC) in WB
Expression level of fatty acid transporters (CD36 and CPT1-M), LCAD in WT. ...View More
Maurya, S. K., Herrera, J. L., Sahoo, S. K., Reis, F. C., Vega, R. B., Kelly, D. P., & Periasamy, M. (2018). Sarcolipin signaling promotes mitochondrial biogenesis and oxidative metabolism in skeletal muscle. Cell reports, 24(11), 2919-2931.

Summary

Host Animal
Mouse
Specificity
Mouse, Rat
Clone
V2-179171
Antibody Isotype
IgG1
Application
WB, IP

Basic Information

Immunogen
Tissue, cells or virus.
Specificity
Mouse, Rat
Antibody Isotype
IgG1
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1 µg/ml

Formulations & Storage [For reference only, actual COA shall prevail!]

Buffer
HEPES, pH 7.5
Preservative
0.02% sodium azide
Concentration
Batch dependent
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Acyl-CoA Dehydrogenase Long Chain
Introduction
ACADL belongs to the acyl-CoA dehydrogenase family, which is a family of mitochondrial flavoenzymes involved in fatty acid and branched chain amino-acid metabolism. This protein is one of the four enzymes that catalyze the initial step of mitochondrial be
Entrez Gene ID
Mouse11363
Rat25287
UniProt ID
MouseP51174
RatP15650
Alternative Names
Acyl-CoA Dehydrogenase Long Chain; Acyl-Coenzyme A Dehydrogenase, Long Chain; LCAD; Long-Chain Specific Acyl-CoA Dehydrogenase, Mitochondrial; EC 1.3.99.13; EC 1.3.8.8; EC 1.3.99; ACAD4;
Function
Long-chain specific acyl-CoA dehydrogenase is one of the acyl-CoA dehydrogenases that catalyze the first step of mitochondrial fatty acid beta-oxidation, an aerobic process breaking down fatty acids into acetyl-CoA and allowing the production of energy from fats (By similarity). The first step of fatty acid beta-oxidation consists in the removal of one hydrogen from C-2 and C-3 of the straight-chain fatty acyl-CoA thioester, resulting in the formation of trans-2-enoyl-CoA (By similarity). Among the different mitochondrial acyl-CoA dehydrogenases, long-chain specific acyl-CoA dehydrogenase can act on saturated and unsaturated acyl-CoAs with 6 to 24 carbons with a preference for 8 to 18 carbons long primary chains.
Biological Process
Carnitine catabolic process
Carnitine metabolic process, CoA-linked
Cellular lipid catabolic process
Fatty acid beta-oxidation
Fatty acid beta-oxidation using acyl-CoA dehydrogenase
Long-chain fatty acid catabolic process
Negative regulation of fatty acid biosynthetic process
Negative regulation of fatty acid oxidation
Positive regulation of cold-induced thermogenesis
Regulation of cholesterol metabolic process
Temperature homeostasis
Cellular Location
Mitochondrion matrix
PTM
Acetylation at Lys-318 and Lys-322 in proximity of the cofactor-binding sites strongly reduces catalytic activity. These sites are deacetylated by SIRT3.

Beck, M. E., Zhang, Y., Bharathi, S. S., Kosmider, B., Bahmed, K., Dahmer, M. K., ... & Goetzman, E. S. (2020). The common K333Q polymorphism in long-chain acyl-CoA dehydrogenase (LCAD) reduces enzyme stability and function. Molecular genetics and metabolism, 131(1-2), 83-89.

Zhang, M., Sunaba, T., Sun, Y., Shibata, T., Sasaki, K., Isoda, H., ... & Kita, M. (2020). Acyl-CoA dehydrogenase long chain (ACADL) is a target protein of stylissatin A, an anti-inflammatory cyclic heptapeptide. The Journal of antibiotics, 73(8), 589-592.

Mezhnina, V., Pearce, R., Poe, A., Velingkaar, N., Astafev, A., Ebeigbe, O. P., ... & Kondratov, R. V. (2020). CR reprograms acetyl‐CoA metabolism and induces long‐chain acyl‐CoA dehydrogenase and CrAT expression. Aging cell, 19(11), e13266.

Ranea‐Robles, P., Yu, C., van Vlies, N., Vaz, F. M., & Houten, S. M. (2020). Slc22a5 haploinsufficiency does not aggravate the phenotype of the long‐chain acyl‐CoA dehydrogenase KO mouse. Journal of inherited metabolic disease, 43(3), 486-495.

Gaston, G., Gangoiti, J. A., Winn, S., Chan, B., Barshop, B. A., Harding, C. O., & Gillingham, M. B. (2020). Cardiac tissue citric acid cycle intermediates in exercised very long‐chain acyl‐CoA dehydrogenase‐deficient mice fed triheptanoin or medium‐chain triglyceride. Journal of inherited metabolic disease, 43(6), 1232-1242.

Zhang, Y., Bharathi, S. S., Beck, M. E., & Goetzman, E. S. (2019). The fatty acid oxidation enzyme long-chain acyl-CoA dehydrogenase can be a source of mitochondrial hydrogen peroxide. Redox biology, 26, 101253.

Yu, D. L., Li, H. W., Wang, Y., Li, C. Q., You, D., Jiang, L., ... & Li, X. H. (2018). Acyl-CoA dehydrogenase long chain expression is associated with esophageal squamous cell carcinoma progression and poor prognosis. OncoTargets and therapy, 11, 7643.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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