Mouse Acadl ELISA Kit (V2LY-0626-LY2545)

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Tested Data
Datasheet
SDS
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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Sensitivity
0.0016 ng/mL
Detection Range
0.003-0.9 ng/mL
Sample Type
Serum, Plasma, cell culture supernates
Specificity
Mouse
Assay Type
Sandwich
Reactivity
Mouse
Assay Time
1.5 h
Molecule Mass
47.9 kDa
Components
  • Pre-coated ELISA plate: 12 wells * 8 detachable strips
  • Standard solution: 0.5ml x1
  • Standard diluent: 3ml x1
  • Streptavidin-HRP: 6ml x1
  • Stop solution: 6ml x1
  • Substrate solution A: 6ml x1
  • Substrate solution B: 6ml x1
  • Wash buffer concentrate (25x): 20ml x1
  • Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 2-8°C
More Infomation

Target

Full Name
Acyl-CoA Dehydrogenase Long Chain
Function
Long-chain specific acyl-CoA dehydrogenase is one of the acyl-CoA dehydrogenases that catalyze the first step of mitochondrial fatty acid beta-oxidation, an aerobic process breaking down fatty acids into acetyl-CoA and allowing the production of energy from fats (By similarity). The first step of fatty acid beta-oxidation consists in the removal of one hydrogen from C-2 and C-3 of the straight-chain fatty acyl-CoA thioester, resulting in the formation of trans-2-enoyl-CoA (By similarity). Among the different mitochondrial acyl-CoA dehydrogenases, long-chain specific acyl-CoA dehydrogenase can act on saturated and unsaturated acyl-CoAs with 6 to 24 carbons with a preference for 8 to 18 carbons long primary chains.
Biological Process
Carnitine catabolic process
Carnitine metabolic process, CoA-linked
Cellular lipid catabolic process
Fatty acid beta-oxidation
Fatty acid beta-oxidation using acyl-CoA dehydrogenase
Long-chain fatty acid catabolic process
Negative regulation of fatty acid biosynthetic process
Negative regulation of fatty acid oxidation
Positive regulation of cold-induced thermogenesis
Regulation of cholesterol metabolic process
Temperature homeostasis
Cellular Location
Mitochondrion matrix
PTM
Acetylation at Lys-318 and Lys-322 in proximity of the cofactor-binding sites strongly reduces catalytic activity. These sites are deacetylated by SIRT3.

Beck, M. E., Zhang, Y., Bharathi, S. S., Kosmider, B., Bahmed, K., Dahmer, M. K., ... & Goetzman, E. S. (2020). The common K333Q polymorphism in long-chain acyl-CoA dehydrogenase (LCAD) reduces enzyme stability and function. Molecular genetics and metabolism, 131(1-2), 83-89.

Zhang, M., Sunaba, T., Sun, Y., Shibata, T., Sasaki, K., Isoda, H., ... & Kita, M. (2020). Acyl-CoA dehydrogenase long chain (ACADL) is a target protein of stylissatin A, an anti-inflammatory cyclic heptapeptide. The Journal of antibiotics, 73(8), 589-592.

Mezhnina, V., Pearce, R., Poe, A., Velingkaar, N., Astafev, A., Ebeigbe, O. P., ... & Kondratov, R. V. (2020). CR reprograms acetyl‐CoA metabolism and induces long‐chain acyl‐CoA dehydrogenase and CrAT expression. Aging cell, 19(11), e13266.

Ranea‐Robles, P., Yu, C., van Vlies, N., Vaz, F. M., & Houten, S. M. (2020). Slc22a5 haploinsufficiency does not aggravate the phenotype of the long‐chain acyl‐CoA dehydrogenase KO mouse. Journal of inherited metabolic disease, 43(3), 486-495.

Gaston, G., Gangoiti, J. A., Winn, S., Chan, B., Barshop, B. A., Harding, C. O., & Gillingham, M. B. (2020). Cardiac tissue citric acid cycle intermediates in exercised very long‐chain acyl‐CoA dehydrogenase‐deficient mice fed triheptanoin or medium‐chain triglyceride. Journal of inherited metabolic disease, 43(6), 1232-1242.

Zhang, Y., Bharathi, S. S., Beck, M. E., & Goetzman, E. S. (2019). The fatty acid oxidation enzyme long-chain acyl-CoA dehydrogenase can be a source of mitochondrial hydrogen peroxide. Redox biology, 26, 101253.

Yu, D. L., Li, H. W., Wang, Y., Li, C. Q., You, D., Jiang, L., ... & Li, X. H. (2018). Acyl-CoA dehydrogenase long chain expression is associated with esophageal squamous cell carcinoma progression and poor prognosis. OncoTargets and therapy, 11, 7643.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Mouse Anti-ACADL Recombinant Antibody (V2-179171) (CAT#: CBMAB-A0428-YC)

Isotype control

For research use only. Not intended for any clinical use.

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We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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