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Mouse Anti-ACAT1 Recombinant Antibody (V2-179213) (CBMAB-A0474-YC)

Provided herein is a Mouse monoclonal antibody against Human Acetyl-CoA Acetyltransferase 1. The antibody can be used for immunoassay techniques, such as ELISA, ICC, IHC, IP.
See all ACAT1 antibodies
Published Data
Mouse Anti-ACAT1 Recombinant Antibody (9H10AB4) (CBMAB-A0474-YC) in WB
...View More
Guan, J., Jiang, X., Gai, J., Sun, X., Zhao, J., Li, J., ... & Li, Q. (2020). Sirtuin 5 regulates the proliferation, invasion and migration of prostate cancer cells through acetyl-CoA acetyltransferase 1. Journal of cellular and molecular medicine, 24(23), 14039-14049.
Mouse Anti-ACAT1 Recombinant Antibody (9H10AB4) (CBMAB-A0474-YC) in IF
Confocal experiments show that ACAT1 protein in LNCaP cells. ...View More
Guan, J., Jiang, X., Gai, J., Sun, X., Zhao, J., Li, J., ... & Li, Q. (2020). Sirtuin 5 regulates the proliferation, invasion and migration of prostate cancer cells through acetyl-CoA acetyltransferase 1. Journal of cellular and molecular medicine, 24(23), 14039-14049.

Summary

Host Animal
Mouse
Specificity
Human
Clone
V2-179213
Antibody Isotype
IgG2a
Application
ELISA, ICC, IHC, IP

Basic Information

Immunogen
Human liver mitochondria
Specificity
Human
Antibody Isotype
IgG2a
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Buffer
PBS, pH7.4
Preservative
None
Concentration
Batch dependent
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Acetyl-CoA Acetyltransferase 1
Introduction
ACAT1 is a mitochondrially localized enzyme that catalyzes the reversible formation of acetoacetyl-CoA from two molecules of acetyl-CoA. Defects in this gene are associated with 3-ketothiolase deficiency, an inborn error of isoleucine catabolism character
Entrez Gene ID
38
UniProt ID
P24752
Alternative Names
Acetyl-CoA Acetyltransferase 1; Acetyl-Coenzyme A Acetyltransferase 1; Acetoacetyl Coenzyme A Thiolase; Acetoacetyl-CoA Thiolase; EC 2.3.1.9; ACAT; MAT;
Function
This is one of the enzymes that catalyzes the last step of the mitochondrial beta-oxidation pathway, an aerobic process breaking down fatty acids into acetyl-CoA. Using free coenzyme A/CoA, catalyzes the thiolytic cleavage of medium- to long-chain 3-oxoacyl-CoAs into acetyl-CoA and a fatty acyl-CoA shortened by two carbon atoms. The activity of the enzyme is reversible and it can also catalyze the condensation of two acetyl-CoA molecules into acetoacetyl-CoA. Thereby, it plays a major role in ketone body metabolism.
Biological Process
Acetyl-CoA biosynthetic process
Acetyl-CoA catabolic process
Branched-chain amino acid catabolic process
Cholesterol esterification
Coenzyme A biosynthetic process
Coenzyme A metabolic process
Fatty acid beta-oxidation
Isoleucine catabolic process
Ketone body biosynthetic process
Ketone body catabolic process
Ketone body metabolic process
Propionyl-CoA biosynthetic process
Cellular Location
Mitochondrion
Involvement in disease
An autosomal recessive inborn error of isoleucine catabolism characterized by intermittent ketoacidotic attacks associated with unconsciousness. Some patients die during an attack or are mentally retarded. Urinary excretion of 2-methyl-3-hydroxybutyric acid, 2-methylacetoacetic acid, triglylglycine, butanone is increased. It seems likely that the severity of this disease correlates better with the environmental or acquired factors than with the ACAT1 genotype.
PTM
Succinylation at Lys-268, adjacent to a coenzyme A binding site. Desuccinylated by SIRT5 (By similarity).

Guan, J., Jiang, X., Gai, J., Sun, X., Zhao, J., Li, J., ... & Li, Q. (2020). Sirtuin 5 regulates the proliferation, invasion and migration of prostate cancer cells through acetyl‐CoA acetyltransferase 1. Journal of cellular and molecular medicine, 24(23), 14039-14049.

Lu, Y., Zhou, X., Zhao, W., Liao, Z., Li, B., Yang, Y., ... & Zhou, X. (2020). Epigenetic Inactivation of Acetyl-Co A acetyltransferase 1 promotes the proliferation and metastasis of nasopharyngeal carcinoma via decreasing ketogenesis.

Chen, L., Peng, T., Luo, Y., Zhou, F., Wang, G., Qian, K., ... & Wang, X. (2019). ACAT1 and metabolism-related pathways are essential for the progression of clear cell renal cell carcinoma (ccRCC), as determined by co-expression network analysis. Frontiers in oncology, 9, 957.

Bi, M., Qiao, X., Zhang, H., Wu, H., Gao, Z., Zhou, H., ... & Zhao, Z. (2019). Effect of inhibiting ACAT‑1 expression on the growth and metastasis of Lewis lung carcinoma. Oncology letters, 18(2), 1548-1556.

Goudarzi, A. (2019). The recent insights into the function of ACAT1: A possible anti-cancer therapeutic target. Life sciences, 232, 116592.

Chanyshev, M. D., Razumova, Y. V., Ovchinnikov, V. Y., & Gulyaeva, L. F. (2018). MiR-21 regulates the ACAT1 gene in MCF-7 cells. Life sciences, 209, 173-178.

Kern, R. J., Zarek, C. M., Lindholm‐Perry, A. K., Kuehn, L. A., Snelling, W. M., Freetly, H. C., ... & Meyer, A. M. (2017). Ruminal expression of the NQO 1, RGS 5, and ACAT 1 genes may be indicators of feed efficiency in beef steers. Animal genetics, 48(1), 90-92.

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For research use only. Not intended for any clinical use.

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We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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