Mouse Anti-ACAT1 Recombinant Antibody (V2-179214) (CBMAB-A0475-YC)

Name: Mouse Anti-ACAT1 Recombinant Antibody (V2-179214)
SKU: CBMAB-A0475-YC
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

V2-179214

Application

ELISA, IHC-P, WB

Immunogen

N-terminal 104 amino acids of human ACAT1 protein.

Specificity

Human

Antibody Isotype

IgG

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:1,000
ELISA	1:2,000-1:5,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Buffer

PBS, pH7.4

Preservative

0.05% sodium azide

Concentration

Batch dependent

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Epitope

N-terminus

More Infomation

Target

Full Name

Acetyl-CoA Acetyltransferase 1

Introduction

ACAT1 is a mitochondrially localized enzyme that catalyzes the reversible formation of acetoacetyl-CoA from two molecules of acetyl-CoA. Defects in this gene are associated with 3-ketothiolase deficiency, an inborn error of isoleucine catabolism character

Entrez Gene ID

UniProt ID

P24752

Alternative Names

Acetyl-CoA Acetyltransferase 1; Acetyl-Coenzyme A Acetyltransferase 1; Acetoacetyl Coenzyme A Thiolase; Acetoacetyl-CoA Thiolase; EC 2.3.1.9; ACAT; MAT;

Function

This is one of the enzymes that catalyzes the last step of the mitochondrial beta-oxidation pathway, an aerobic process breaking down fatty acids into acetyl-CoA. Using free coenzyme A/CoA, catalyzes the thiolytic cleavage of medium- to long-chain 3-oxoacyl-CoAs into acetyl-CoA and a fatty acyl-CoA shortened by two carbon atoms. The activity of the enzyme is reversible and it can also catalyze the condensation of two acetyl-CoA molecules into acetoacetyl-CoA. Thereby, it plays a major role in ketone body metabolism.

Biological Process

Acetyl-CoA biosynthetic process
Acetyl-CoA catabolic process
Branched-chain amino acid catabolic process
Cholesterol esterification
Coenzyme A biosynthetic process
Coenzyme A metabolic process
Fatty acid beta-oxidation
Isoleucine catabolic process
Ketone body biosynthetic process
Ketone body catabolic process
Ketone body metabolic process
Propionyl-CoA biosynthetic process

Cellular Location

Mitochondrion

Involvement in disease

An autosomal recessive inborn error of isoleucine catabolism characterized by intermittent ketoacidotic attacks associated with unconsciousness. Some patients die during an attack or are mentally retarded. Urinary excretion of 2-methyl-3-hydroxybutyric acid, 2-methylacetoacetic acid, triglylglycine, butanone is increased. It seems likely that the severity of this disease correlates better with the environmental or acquired factors than with the ACAT1 genotype.

PTM

Succinylation at Lys-268, adjacent to a coenzyme A binding site. Desuccinylated by SIRT5 (By similarity).

Guan, J., Jiang, X., Gai, J., Sun, X., Zhao, J., Li, J., ... & Li, Q. (2020). Sirtuin 5 regulates the proliferation, invasion and migration of prostate cancer cells through acetyl‐CoA acetyltransferase 1. Journal of cellular and molecular medicine, 24(23), 14039-14049.

Lu, Y., Zhou, X., Zhao, W., Liao, Z., Li, B., Yang, Y., ... & Zhou, X. (2020). Epigenetic Inactivation of Acetyl-Co A acetyltransferase 1 promotes the proliferation and metastasis of nasopharyngeal carcinoma via decreasing ketogenesis.

Chen, L., Peng, T., Luo, Y., Zhou, F., Wang, G., Qian, K., ... & Wang, X. (2019). ACAT1 and metabolism-related pathways are essential for the progression of clear cell renal cell carcinoma (ccRCC), as determined by co-expression network analysis. Frontiers in oncology, 9, 957.

Bi, M., Qiao, X., Zhang, H., Wu, H., Gao, Z., Zhou, H., ... & Zhao, Z. (2019). Effect of inhibiting ACAT‑1 expression on the growth and metastasis of Lewis lung carcinoma. Oncology letters, 18(2), 1548-1556.

Goudarzi, A. (2019). The recent insights into the function of ACAT1: A possible anti-cancer therapeutic target. Life sciences, 232, 116592.

Chanyshev, M. D., Razumova, Y. V., Ovchinnikov, V. Y., & Gulyaeva, L. F. (2018). MiR-21 regulates the ACAT1 gene in MCF-7 cells. Life sciences, 209, 173-178.

Kern, R. J., Zarek, C. M., Lindholm‐Perry, A. K., Kuehn, L. A., Snelling, W. M., Freetly, H. C., ... & Meyer, A. M. (2017). Ruminal expression of the NQO 1, RGS 5, and ACAT 1 genes may be indicators of feed efficiency in beef steers. Animal genetics, 48(1), 90-92.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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For research use only. Not intended for any clinical use.

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