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Mouse Anti-ACBD3 Recombinant Antibody (V2-179238) (CBMAB-A0505-YC)

Provided herein is a Mouse monoclonal antibody against Human Acyl-CoA Binding Domain Containing 3. The antibody can be used for immunoassay techniques, such as ELISA, WB.
See all ACBD3 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
V2-179238
Antibody Isotype
IgG2a, κ
Application
ELISA, WB

Basic Information

Immunogen
ACBD3 (NP_073572, 73-171 aa) partial recombinant protein with GST tag. Immunogen sequence: RRLEQRWGFG LEELYGLALR FFKEKDGKAF HPTYEEKLKL VALHKQVLMG PYNPDTCPEV GFFDVLGNDR RREWAALGNM SKEDAMVEFV KLLNRCCHL
Specificity
Human
Antibody Isotype
IgG2a, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Buffer
PBS, pH7.4
Preservative
None
Concentration
Batch dependent
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
acyl-Coenzyme A binding domain containing 3
Introduction
The Golgi complex plays a key role in the sorting and modification of proteins exported from the endoplasmic reticulum. The protein encoded by this gene is involved in the maintenance of Golgi structure and function through its interaction with the integr
Entrez Gene ID
64746
UniProt ID
Q9H3P7
Alternative Names
Acyl-CoA Binding Domain Containing 3; PBR- And PKA-Associated Protein 7; Peripheral Benzodiazepine Receptor-Associated Protein PAP7; Acyl-Coenzyme A Binding Domain Containing 3; Golgi Complex Associated Protein 1, 60kDa; Golgi Phosphoprotein 1; GOCAP1; GO
Function
Involved in the maintenance of Golgi structure by interacting with giantin, affecting protein transport between the endoplasmic reticulum and Golgi. Involved in hormone-induced steroid biosynthesis in testicular Leydig cells (By similarity). Recruits PI4KB to the Golgi apparatus membrane; enhances the enzyme activity of PI4KB activity via its membrane recruitment thereby increasing the local concentration of the substrate in the vicinity of the kinase.
Biological Process
Post-Golgi vesicle-mediated transport
Steroid biosynthetic process
Viral process
Cellular Location
Golgi apparatus membrane; Mitochondrion. Also mitochondrial (via its interaction with PBR).

Shin, H. J., Ku, K. B., Kim, S., Kim, H. S., Kim, Y. S., Kim, B. T., ... & Kim, C. (2021). A Crucial Role of ACBD3 Required for Coxsackievirus Infection in Animal Model Developed by AAV-Mediated CRISPR Genome Editing Technique. Viruses, 13(2), 237.

Daňhelovská, T., Zdražilová, L., Štufková, H., Vanišová, M., Volfová, N., Křížová, J., ... & Tesařová, M. (2021). Knock-Out of ACBD3 Leads to Dispersed Golgi Structure, but Unaffected Mitochondrial Functions in HEK293 and HeLa Cells. International Journal of Molecular Sciences, 22(14), 7270.

Lu, Y., Yang, X., & Zhang, L. (2020). Domain I of hepatitis C virus NS5A associates with ACBD3 in a genotype‐dependent manner. Microbiology and immunology, 64(8), 574-577.

Lu, Y., Song, S., & Zhang, L. (2020). Emerging Role for Acyl-CoA Binding Domain Containing 3 at Membrane Contact Sites During Viral Infection. Frontiers in microbiology, 11, 608.

Yue, X., Qian, Y., Gim, B., & Lee, I. (2019). Acyl-CoA-binding domain-containing 3 (ACBD3; PAP7; GCP60): a multi-functional membrane domain organizer. International journal of molecular sciences, 20(8), 2028.

Lyoo, H., Van Der Schaar, H. M., Dorobantu, C. M., Rabouw, H. H., Strating, J. R., & Van Kuppeveld, F. J. (2019). ACBD3 is an essential pan-enterovirus host factor that mediates the interaction between viral 3A protein and cellular protein PI4KB. MBio, 10(1), e02742-18.

Liao, J., Guan, Y., Chen, W., Shi, C., Yao, D., Wang, F., ... & Cao, X. (2019). ACBD3 is required for FAPP2 transferring glucosylceramide through maintaining the Golgi integrity. Journal of molecular cell biology, 11(2), 107-117.

Huang, Y., Yang, L., Pei, Y. Y., Wang, J., Wu, H., Yuan, J., & Wang, L. (2018). Overexpressed ACBD3 has prognostic value in human breast cancer and promotes the self-renewal potential of breast cancer cells by activating the Wnt/beta-catenin signaling pathway. Experimental cell research, 363(1), 39-47.

Klima, M., Chalupska, D., Różycki, B., Humpolickova, J., Rezabkova, L., Silhan, J., ... & Boura, E. (2017). Kobuviral non-structural 3A proteins act as molecular harnesses to hijack the host ACBD3 protein. Structure, 25(2), 219-230.

Yue, X., Bao, M., Christiano, R., Li, S., Mei, J., Zhu, L., ... & Lee, I. (2017). ACBD 3 functions as a scaffold to organize the Golgi stacking proteins and a Rab33b‐GAP. FEBS letters, 591(18), 2793-2802.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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