Rabbit Anti-ACLY Recombinant Antibody (
3A5) (V2LY-0725-LY1342)

Name: Rabbit Anti-ACLY Recombinant Antibody (3A5)
SKU: V2LY-0725-LY1342
Rating: 5 (1 reviews)

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Tested Data

Rabbit Anti-ACLY Recombinant Antibody (3A5) (V2LY-0725-LY1342) in WB

Western Blot
Positive WB detected in: L02 whole cell lysate, K562 whole cell lysate, Hela whole cell lysate, Raji whole cell lysate, A549 whole cell lysate
All lanes: ACLY antibody at 1:1500
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 121, 120, 92 kDa
Observed band size: 120 kDa

Rabbit Anti-ACLY Recombinant Antibody (3A5) (V2LY-0725-LY1342) in IF

Immunofluorescence staining of HepG2 Cells with V2LY-0725-LY1342 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).

Rabbit Anti-ACLY Recombinant Antibody (3A5) (V2LY-0725-LY1342) in FC

Overlay histogram showing Hela cells stained with V2LY-0725-LY1342 (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then incubated in 10% normal goat serum to block non-specific protein-protein interactions followedby the antibody (1µg/1*106 cells) for 1 h at 4°C.The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4°C. Control antibody (green line) was Rabbit IgG (1µg/1*106 cells) used under the same conditions. Acquisition of>10,000 events was performed.

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Rabbit

Clone

3A5

Application

ELISA, WB, IF, FC

Immunogen

A synthesized peptide.

Host Species

Rabbit

Specificity

Human

Antibody Isotype

IgG

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:500-1:5,000
IF	1:20-1:200
FC	1:20-1:200

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, glycerol

Preservative

Sodium azide

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

ATP Citrate Lyase

Entrez Gene ID

UniProt ID

P53396

Function

Catalyzes the cleavage of citrate into oxaloacetate and acetyl-CoA, the latter serving as common substrate for de novo cholesterol and fatty acid synthesis.

Biological Process

Acetyl-CoA biosynthetic process
Cholesterol biosynthetic process
Citrate metabolic process
Coenzyme A metabolic process
Fatty acid biosynthetic process
Fatty-acyl-CoA biosynthetic process
Lipid biosynthetic process
Neutrophil degranulation
Oxaloacetate metabolic process
Positive regulation of cellular metabolic process

Cellular Location

Cytosol

PTM

Phosphorylated by PKA and GSK3 in a sequential manner; phosphorylation results in activation of its activity. Phosphorylation on Thr-447 and Ser-451 depends on the phosphorylation state of Ser-455 (By similarity). Phosphorylation on Ser-455 is decreased by prior phosphorylation on the other 2 residues (By similarity).
ISGylated.
Acetylated at Lys-540, Lys-546 and Lys-554 by KAT2B/PCAF. Acetylation is promoted by glucose and stabilizes the protein, probably by preventing ubiquitination at the same sites. Acetylation promotes de novo lipid synthesis. Deacetylated by SIRT2.
Ubiquitinated at Lys-540, Lys-546 and Lys-554 by UBR4, leading to its degradation. Ubiquitination is probably inhibited by acetylation at same site (Probable).

Dominguez, M., Brüne, B., & Namgaladze, D. (2021). Exploring the Role of ATP-Citrate Lyase in the Immune System. Frontiers in Immunology, 12, 14.

Han, Q., Chen, C. A., Yang, W., Liang, D., Lv, H. W., Lv, G. S., ... & Wang, H. Y. (2020). ATP-citrate lyase regulates stemness and metastasis in hepatocellular carcinoma via the Wnt/β-catenin signaling pathway. Hepatobiliary & Pancreatic Diseases International.

Feng, X., Zhang, L., Xu, S., & Shen, A. Z. (2020). ATP-citrate lyase (ACLY) in lipid metabolism and atherosclerosis: an updated review. Progress in lipid research, 77, 101006.

Icard, P., Wu, Z., Fournel, L., Coquerel, A., Lincet, H., & Alifano, M. (2020). ATP citrate lyase: a central metabolic enzyme in cancer. Cancer letters, 471, 125-134.

Kumari, R., Deshmukh, R. S., & Das, S. (2019). Caspase-10 inhibits ATP-citrate lyase-mediated metabolic and epigenetic reprogramming to suppress tumorigenesis. Nature communications, 10(1), 1-15.

Göttgens, E. L., van den Heuvel, C. N., de Jong, M. C., Kaanders, J. H., Leenders, W. P., Ansems, M., ... & Span, P. N. (2019). ACLY (ATP citrate lyase) mediates radioresistance in head and neck squamous cell carcinomas and is a novel predictive radiotherapy biomarker. Cancers, 11(12), 1971.

Wei, J., Leit, S., Kuai, J., Therrien, E., Rafi, S., Harwood, H. J., ... & Tong, L. (2019). An allosteric mechanism for potent inhibition of human ATP-citrate lyase. Nature, 568(7753), 566-570.

Granchi, C. (2018). ATP citrate lyase (ACLY) inhibitors: An anti-cancer strategy at the crossroads of glucose and lipid metabolism. European journal of medicinal chemistry, 157, 1276-1291.

Namgaladze, D., Zukunft, S., Schnütgen, F., Kurrle, N., Fleming, I., Fuhrmann, D., & Brüne, B. (2018). Polarization of human macrophages by interleukin-4 does not require ATP-citrate lyase. Frontiers in immunology, 9, 2858.

Teng, L., Chen, Y., Cao, Y., Wang, W., Xu, Y., Wang, Y., ... & Su, Y. (2018). Overexpression of ATP citrate lyase in renal cell carcinoma tissues and its effect on the human renal carcinoma cells in vitro. Oncology letters, 15(5), 6967-6974.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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