ACLY

ATP citrate lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. The enzyme is a tetramer (relative molecular weight approximately 440,000) of apparently identical subunits. It catalyzes the formation of acetyl-CoA and oxaloacetate from citrate and CoA with a concomitant hydrolysis of ATP to ADP and phosphate. The product, acetyl-CoA, serves several important biosynthetic pathways, including lipogenesis and cholesterogenesis. In nervous tissue, ATP citrate-lyase may be involved in the biosynthesis of acetylcholine. Multiple transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Dec 2014]

Full Name

ATP Citrate Lyase

Function

Catalyzes the cleavage of citrate into oxaloacetate and acetyl-CoA, the latter serving as common substrate for de novo cholesterol and fatty acid synthesis.

Biological Process

Acetyl-CoA biosynthetic process
Cholesterol biosynthetic process
Citrate metabolic process
Coenzyme A metabolic process
Fatty acid biosynthetic process
Fatty-acyl-CoA biosynthetic process
Lipid biosynthetic process
Neutrophil degranulation
Oxaloacetate metabolic process
Positive regulation of cellular metabolic process

Cellular Location

Cytosol

PTM

Phosphorylated by PKA and GSK3 in a sequential manner; phosphorylation results in activation of its activity. Phosphorylation on Thr-447 and Ser-451 depends on the phosphorylation state of Ser-455 (By similarity). Phosphorylation on Ser-455 is decreased by prior phosphorylation on the other 2 residues (By similarity).
ISGylated.
Acetylated at Lys-540, Lys-546 and Lys-554 by KAT2B/PCAF. Acetylation is promoted by glucose and stabilizes the protein, probably by preventing ubiquitination at the same sites. Acetylation promotes de novo lipid synthesis. Deacetylated by SIRT2.
Ubiquitinated at Lys-540, Lys-546 and Lys-554 by UBR4, leading to its degradation. Ubiquitination is probably inhibited by acetylation at same site (Probable).

Anti-ACLY antibodies

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Mouse Anti-ACLY Recombinant Antibody (V2-179314) (CBMAB-A0610-YC)

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Target: ACLY

Host: Mouse

Antibody Isotype: IgG1

Specificity: Human, Mouse, Monkey, Rat

Clone: V2-179314

Application*: E, F, IC, IF, IH, P, WB

Datasheet SDS

Mouse Anti-ACLY Recombinant Antibody (V2-631896) (CBMAB-CA080LY)

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Target: ACLY

Host: Mouse

Antibody Isotype: IgG2a

Specificity: Human, Mouse, Rat, Pig

Clone: V2-631896

Application*: ELISA, WB, ICC, FC

Datasheet SDS

Mouse Anti-ACLY Recombinant Antibody (V2-630606) (CBMAB-BR003LY)

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Target: ACLY

Host: Mouse

Antibody Isotype: IgG2b

Specificity: Human, Mouse, Rat

Clone: V2-630606

Application*: FC, IHC-P, WB

Datasheet SDS

Rabbit Anti-ACLY Recombinant Antibody (V2-179319) (CBMAB-A0615-YC)

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Target: ACLY

Host: Rabbit

Antibody Isotype: IgG

Specificity: Human

Clone: V2-179319

Application*: IC, IF

Datasheet SDS

Mouse Anti-ACLY Recombinant Antibody (V2-179313) (CBMAB-A0609-YC)

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Target: ACLY

Host: Mouse

Antibody Isotype: IgG1

Specificity: Human

Clone: V2-179313

Application*: IC, IF, IH, P, WB

Datasheet SDS

Mouse Anti-ACLY Recombinant Antibody (V2-179312) (CBMAB-A0608-YC)

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Target: ACLY

Host: Mouse

Antibody Isotype: IgG2a

Specificity: Human

Clone: V2-179312

Application*: F, IC, IF, WB

Datasheet SDS

Mouse Anti-ACLY Recombinant Antibody (V2-179310) (CBMAB-A0606-YC)

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Target: ACLY

Host: Mouse

Antibody Isotype: IgG2a

Specificity: Human, Monkey, Mouse

Clone: V2-179310

Application*: F, IC, WB

Datasheet SDS

Mouse Anti-ACLY Recombinant Antibody (V2-179309) (CBMAB-A0605-YC)

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Target: ACLY

Host: Mouse

Antibody Isotype: IgG1

Specificity: Human, Monkey, Mouse

Clone: V2-179309

Application*: E, F, IC, IH, WB

Datasheet SDS

Human Recombinant ACLY protein, His Tag (V2LY-0526-LY2199)

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Target: ACLY

Expressed Host: Baculovirus-Insect Cells

Sequence: Amino Acid: 1-1101

Tag: His Tag

Datasheet SDS

Rabbit Anti-ACLY Recombinant Antibody (
3A5) (V2LY-0725-LY1342)

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Target: ACLY

Host: Rabbit

Antibody Isotype: IgG

Specificity: Human

Clone: 3A5

Application*: E, WB, IF, F

Datasheet SDS

Mouse Anti-ACLY Recombinant Antibody (
11H7) (V2LY-0725-LY290)

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Target: ACLY

Host: Mouse

Antibody Isotype: IgG2a

Specificity: Human, Mouse, Rat, Pig

Clone: 11H7

Application*: E, WB, IC, F

Datasheet SDS

Mouse Anti-ACLY Recombinant Antibody (CBT196) (V2LY-0625-LY3717)

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Target: ACLY

Host: Mouse

Antibody Isotype: IgG

Specificity: Human, Mouse, Rat, Cattle, Chicken, Pig, Sheep

Clone: CBT196

Application*: WB, IF, IC, F

Datasheet SDS

Mouse Anti-ACLY Recombinant Antibody (CBT3317) (V2LY-0625-LY2791)

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Target: ACLY

Host: Mouse

Antibody Isotype: IgG1

Specificity: Human, Mouse, Monkey, Rat

Clone: CBT3317

Application*: WB, IH, IC, F

Datasheet SDS

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Fig.3 Signaling pathways in cancers. (Creative Biolabs Authorized)

Fig.4 Protocols troubleshootings & guides. (Creative Biolabs Authorized)

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(P): Predicted

* Abbreviations

AActivation
AGAgonist
APApoptosis
BBlocking
BABioassay
BIBioimaging
CImmunohistochemistry-Frozen Sections
CIChromatin Immunoprecipitation
CTCytotoxicity
CSCostimulation
DDepletion
DBDot Blot

EELISA
ECELISA(Cap)
EDELISA(Det)
ESELISpot
EMElectron Microscopy
FFlow Cytometry
FNFunction Assay
GSGel Supershift
IInhibition
IAEnzyme Immunoassay
ICImmunocytochemistry
IDImmunodiffusion
IEImmunoelectrophoresis

IFImmunofluorescence
IGImmunochromatography
IHImmunohistochemistry
IMImmunomicroscopy
IOImmunoassay
IPImmunoprecipitation
ISIntracellular Staining for Flow Cytometry
LALuminex Assay
LFLateral Flow Immunoassay
MMicroarray
MCMass Cytometry/CyTOF
MDMeDIP

MSElectrophoretic Mobility Shift Assay
NNeutralization
PImmunohistologyp-Paraffin Sections
PAPeptide Array
PEPeptide ELISA
PLProximity Ligation Assay
RRadioimmunoassay
SStimulation
SESandwich ELISA
SHIn situ hybridization
TCTissue Culture
WBWestern Blot

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