Has this antibody been validated in species other than human and mouse?

The product datasheet specifies reactivity with human and mouse samples. While recombinant antibodies can sometimes show cross-reactivity with other species if the epitope is conserved, we have not personally tested it on other species. We recommend checking the immunogen sequence homology with your species of interest or running a small pilot experiment.

Can you provide the immunogen sequence for this antibody?

As is standard practice for many antibody companies, the specific immunogen sequence is often proprietary. However, for a recombinant antibody like this, the high specificity is a result of it being generated against a well-defined epitope. For specific collaboration or research needs, it may be possible to obtain more detailed information by contacting the technical support team at Creative Biolabs directly.

Rabbit Anti-ACOD1 Recombinant Antibody (V2-621656) (CBMAB-CP1221-LY)

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Published Data

Rabbit Anti-ACOD1 Recombinant Antibody (D6H2Y) (CBMAB-CP1221-LY) in WB

Fischer, N. L., Boyer, M. A., Bradley, W. P., Spruce, L. A., Fazelinia, H., & Shin, S. (2022). A Coxiella burnetii effector interacts with the host PAF1 complex and suppresses the innate immune response. bioRxiv, 2022-04.

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Rabbit

Clone

V2-621656

Application

WB, IP

Immunogen

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly460 of human IRG1 protein.

Specificity

Human

Antibody Isotype

IgG

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:1,000
IP	1:100

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

HEPES, pH 7.5, 100 µg/ml BSA, 50% glycerol

Preservative

0.02% sodium azide

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Aconitate Decarboxylase 1

Introduction

Involved in the inhibition of the inflammatory response. Acts as a negative regulator of the Toll-like receptors (TLRs)-mediated inflammatory innate response by stimulating the tumor necrosis factor alpha-induced protein TNFAIP3 expression via reactive oxygen species (ROS) in LPS-tolerized macrophages. Involved in antimicrobial response of innate immune cells; ACOD1-mediated itaconic acid production contributes to the antimicrobial activity of macrophages. Plays a role in the embryo implantation.

Entrez Gene ID

730249

UniProt ID

A6NK06

Alternative Names

Aconitate Decarboxylase 1; Cis-Aconitic Acid Decarboxylase; IRG1; CAD; Immune-Responsive Gene 1 Protein Homolog; Immunoresponsive 1 Homolog (Mouse);

Function

Cis-aconitate decarboxylase that catalyzes production of itaconate and is involved in the inhibition of the inflammatory response. Acts as a negative regulator of the Toll-like receptors (TLRs)-mediated inflammatory innate response by stimulating the tumor necrosis factor alpha-induced protein TNFAIP3 expression via reactive oxygen species (ROS) in LPS-tolerized macrophages. Involved in antimicrobial response of innate immune cells; ACOD1-mediated itaconic acid production contributes to the antimicrobial activity of macrophages. Involved in antiviral response following infection by flavivirus in neurons: ACOD1-mediated itaconate production inhibits the activity of succinate dehydrogenase, generating a metabolic state in neurons that suppresses replication of viral genomes (By similarity). Plays a role in the embryo implantation (By similarity).

Biological Process

Cellular response to interferon-beta
Cellular response to interferon-gamma
Cellular response to interleukin-1
Cellular response to lipopolysaccharide
Cellular response to molecule of bacterial origin
Cellular response to progesterone stimulus
Cellular response to tumor necrosis factor
Defense response
Defense response to virus
Embryo implantation
Inflammatory response
Negative regulation of inflammatory response
Negative regulation of innate immune response
Negative regulation of NF-kappaB transcription factor activity
Negative regulation of toll-like receptor 2 signaling pathway
Negative regulation of toll-like receptor 4 signaling pathway
Negative regulation of type I interferon production
Positive regulation of antimicrobial humoral response
Positive regulation of reactive oxygen species metabolic process
Tolerance induction to lipopolysaccharide

Cellular Location

Mitochondrion

Schofield, J. H., Hawk, M. A., Rahmy, S., Sheldon, R. D., Leimbach, C., Sun, S., ... & Schafer, Z. T. (2021). Metabolic evasion of mCRPC immunotherapy by ACOD1 production of itaconate.

Suber, T. L., Olonisakin, T. F., Wendell, S. L., Hulver, M., Xiong, Z., Mallampalli, R. K., & Lee, J. (2020). Aconitate Decarboxylase-1 Deficiency Promotes Bacterial Dissemination During Intrapulmonary Klebsiella Pneumoniae Infection. In D35. LUNG INJURY AND REPAIR: CELLULAR MECHANISMS (pp. A6542-A6542). American Thoracic Society.

Wu, R., Chen, F., Wang, N., Tang, D., & Kang, R. (2020). ACOD1 in immunometabolism and disease. Cellular & Molecular Immunology, 1-12.

Sun, K., Meliton, A. Y., Woods, P. S., Kimmig, L. M., O'Leary, E. M., Wu, D., ... & Mutlu, G. M. (2019). Particulate Matter Induces the Expression of Aconitate Decarboxylase 1, Which by Its Metabolite Itaconate Attenuates Inflammatory Response in Macrophages. In D26. HEALTH EFFECTS ASSOCIATED WITH INDOOR AND OUTDOOR AIR POLLUTION (pp. A6023-A6023). American Thoracic Society.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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