Mouse Anti-ACOT11 Recombinant Antibody (V2-179336) (CBMAB-A0636-YC)

Provided herein is a Mouse monoclonal antibody against Human Acyl-CoA Thioesterase 11. The antibody can be used for immunoassay techniques, such as WB, ELISA, IHC, IHC-P.
See all ACOT11 antibodies

Datasheet

Summary

Host Animal

Mouse

Specificity

Human

Clone

V2-179336

Antibody Isotype

IgG2b

Application

WB, ELISA, IHC, IHC-P

Basic Information

Immunogen

Recombinant human ACOT11 (19-250 aa) purified from E. coli

Specificity

Human

Antibody Isotype

IgG2b

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
ELISA	1:30-1:3,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Buffer

PBS, 0.1% gelatin

Preservative

< 0.1% sodium azide

Concentration

0.1 mg/ml

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name

Acyl-CoA Thioesterase 11

Introduction

ACOT11 is a member of the acyl-CoA thioesterase family which catalyse the conversion of activated fatty acids to the corresponding non-esterified fatty acid and coenzyme A. Expression of a mouse homolog in brown adipose tissue is induced by low temperatur

Entrez Gene ID

26027

UniProt ID

Q8WXI4

Alternative Names

Acyl-CoA Thioesterase 11; StAR-Related Lipid Transfer (START) Domain Containing 14; Thioesterase, Adipose Associated; Brown Fat-Inducible Thioesterase; Acyl-CoA Thioester Hydrolase 11; Adipose-Associated Thioesterase; THEA; BFIT; Thioesterase Superfamily

Function

Has an acyl-CoA thioesterase activity with a preference for the long chain fatty acyl-CoA thioesters hexadecanoyl-CoA/palmitoyl-CoA and tetradecanoyl-CoA/myristoyl-CoA which are the main substrates in the mitochondrial beta-oxidation pathway.

Biological Process

Acyl-CoA metabolic process
Fatty acid metabolic process
Intracellular signal transduction
Negative regulation of cold-induced thermogenesis
Response to cold
Response to temperature stimulus

Cellular Location

Mitochondrion matrix; Cytoplasm

References

Zhang, L., Qiang, J., Tao, Y. F., Bao, J. W., Zhu, H. J., He, J., & Xu, P. (2020). Cloning of the gene encoding acyl-CoA thioesterase 11 and its functional characterization in hybrid yellow catfish (Pelteobagrus fulvidraco♀× Pelteobagrus vachelli♂) under heat stress. Journal of Thermal Biology, 93, 102681.

Xu, C. L., Chen, L., Li, D., Chen, F. T., Sha, M. L., & Shao, Y. (2020). Acyl-CoA Thioesterase 8 and 11 as Novel Biomarkers for Clear Cell Renal Cell Carcinoma. Frontiers in genetics, 11, 1576.

Liang, C., Wang, X., Zhang, Z., Xiao, F., Feng, H., Ma, Q., ... & Liu, D. (2020). ACOT11 promotes cell proliferation, migration and invasion in lung adenocarcinoma. Translational Lung Cancer Research, 9(5), 1885.

Hung, J. Y., Chiang, S. R., Liu, K. T., Tsai, M. J., Huang, M. S., Shieh, J. M., ... & Hsu, Y. L. (2017). Overexpression and proliferation dependence of acyl‑CoA thioesterase 11 and 13 in lung adenocarcinoma. Oncology letters, 14(3), 3647-3656.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Associated Products

Related Products

Mouse Anti-ACOT11 Recombinant Antibody (V2-179335)

Mouse Anti-ACOT11 Recombinant Antibody (V2-179334)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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