Mouse Anti-ACOT9 Recombinant Antibody (V2-179353) (CBMAB-A0654-YC)

Provided herein is a Mouse monoclonal antibody against Human Acyl-CoA Thioesterase 9. The antibody can be used for immunoassay techniques, such as ELISA, ICC, IF, IHC-P, WB.
See all ACOT9 antibodies

Datasheet

Summary

Host Animal

Mouse

Specificity

Human

Clone

V2-179353

Antibody Isotype

IgG1

Application

ELISA, ICC, IF, IHC-P, WB

Basic Information

Immunogen

ACATE2 (AAH12573, 1 a.a. ~ 212 a.a) full-length recombinant protein with GST tag.

Specificity

Human

Antibody Isotype

IgG1

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
IF(ICC)	10 µg/ml
IHC-P	3 µg/ml

Formulations & Storage [For reference only, actual COA shall prevail!]

Buffer

PBS, pH7.4

Preservative

None

Concentration

Batch dependent

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name

Acyl-CoA Thioesterase 9

Introduction

ACOT9 is a mitochondrial acyl-CoA thioesterase of unknown function.

Entrez Gene ID

23597

UniProt ID

Q9Y305

Alternative Names

Acyl-CoA Thioesterase 9; Acyl-CoA Thioester Hydrolase 9; Acyl-Coenzyme A Thioesterase 2, Mitochondrial; Acyl-Coenzyme A Thioesterase 9, Mitochondrial; Mitochondrial Acyl-CoA Thioesterase; EC 3.1.2.15; EC 3.1.2.-;

Function

Acyl-CoA thioesterases are a group of enzymes that catalyze the hydrolysis of acyl-CoAs to the free fatty acid and coenzyme A (CoASH), providing the potential to regulate intracellular levels of acyl-CoAs, free fatty acids and CoASH. Active on long chain acyl-CoAs.

Biological Process

Acyl-CoA metabolic process

Cellular Location

Mitochondrion

References

Jiang, X., & Ding, W. X. (2020). Acyl Coenzyme A Thioesterase 9: A Novel Target for Nonalcoholic Fatty Liver Disease by Shuttling Mitochondrial Short‐Chain Fatty Acids?.

Steensels, S., Qiao, J., Zhang, Y., Maner‐Smith, K. M., Kika, N., Holman, C. D., ... & Ersoy, B. A. (2020). Acyl‐Coenzyme A Thioesterase 9 Traffics Mitochondrial Short‐Chain Fatty Acids Toward De Novo Lipogenesis and Glucose Production in the Liver. Hepatology, 72(3), 857-872.

Bekeova, C., Anderson-Pullinger, L., Boye, K., Boos, F., Sharpadskaya, Y., Herrmann, J. M., & Seifert, E. L. (2019). Multiple mitochondrial thioesterases have distinct tissue and substrate specificity and CoA regulation, suggesting unique functional roles. Journal of Biological Chemistry, 294(50), 19034-19047.

Bekeova, C., Anderson-Pullinger, L., Boye, K., Boos, F., Sharpadskaya, Y., Herrmann, J. M., & Seifert, E. L. (2019). Multiple acyl-CoA thioesterases occupy distinct functional niches within the mitochondrial matrix. bioRxiv, 705327.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Associated Products

Related Products

Mouse Anti-ACOT9 Recombinant Antibody (V2-179354)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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