Mouse Anti-ACOT9 Recombinant Antibody (V2-179354) (CBMAB-A0655-YC)

Name: Mouse Anti-ACOT9 Recombinant Antibody (V2-179354)
SKU: CBMAB-A0655-YC
Rating: 5 (1 reviews)

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Datasheet

Summary

Host Animal

Mouse

Specificity

Human

Clone

V2-179354

Antibody Isotype

IgG3

Application

WB, IP, IF, ELISA

Basic Information

Immunogen

Specific for an epitope mapping between amino acids 11-29 near the N-terminus of ACOT9 of human origin

Specificity

Human

Antibody Isotype

IgG3

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500
ELISA	1:30-1:3,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Buffer

PBS, 0.1% gelatin

Preservative

< 0.1% sodium azide

Concentration

0.2 mg/ml

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name

Acyl-CoA Thioesterase 9

Introduction

ACOT9 is a mitochondrial acyl-CoA thioesterase of unknown function.

Entrez Gene ID

23597

UniProt ID

Q9Y305

Alternative Names

Acyl-CoA Thioesterase 9; Acyl-CoA Thioester Hydrolase 9; Acyl-Coenzyme A Thioesterase 2, Mitochondrial; Acyl-Coenzyme A Thioesterase 9, Mitochondrial; Mitochondrial Acyl-CoA Thioesterase; EC 3.1.2.15; EC 3.1.2.-;

Function

Acyl-CoA thioesterases are a group of enzymes that catalyze the hydrolysis of acyl-CoAs to the free fatty acid and coenzyme A (CoASH), providing the potential to regulate intracellular levels of acyl-CoAs, free fatty acids and CoASH. Active on long chain acyl-CoAs.

Biological Process

Acyl-CoA metabolic process

Cellular Location

Mitochondrion

More Infomation

References

Jiang, X., & Ding, W. X. (2020). Acyl Coenzyme A Thioesterase 9: A Novel Target for Nonalcoholic Fatty Liver Disease by Shuttling Mitochondrial Short‐Chain Fatty Acids?.

Steensels, S., Qiao, J., Zhang, Y., Maner‐Smith, K. M., Kika, N., Holman, C. D., ... & Ersoy, B. A. (2020). Acyl‐Coenzyme A Thioesterase 9 Traffics Mitochondrial Short‐Chain Fatty Acids Toward De Novo Lipogenesis and Glucose Production in the Liver. Hepatology, 72(3), 857-872.

Bekeova, C., Anderson-Pullinger, L., Boye, K., Boos, F., Sharpadskaya, Y., Herrmann, J. M., & Seifert, E. L. (2019). Multiple mitochondrial thioesterases have distinct tissue and substrate specificity and CoA regulation, suggesting unique functional roles. Journal of Biological Chemistry, 294(50), 19034-19047.

Bekeova, C., Anderson-Pullinger, L., Boye, K., Boos, F., Sharpadskaya, Y., Herrmann, J. M., & Seifert, E. L. (2019). Multiple acyl-CoA thioesterases occupy distinct functional niches within the mitochondrial matrix. bioRxiv, 705327.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Associated Products

Related Products

Mouse Anti-ACOT9 Recombinant Antibody (V2-179353)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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Documents

Datasheet
SDS
Request for COA

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Second antibody and isotype control

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