Mouse Anti-ACTC1 Recombinant Antibody (V2-179584) (CBMAB-A0902-YC)

Name: Mouse Anti-ACTC1 Recombinant Antibody (V2-179584)
SKU: CBMAB-A0902-YC
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

V2-179584

Application

ELISA, IHC, WB

Immunogen

Synthetic Nh2 Terminus Decapeptide Of Cardiac Isoform Of Actin

Specificity

Human

Antibody Isotype

IgG1

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:1,000
IHC-P	1:10
IHC-F	1:10

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Lyophilized

Buffer

PBS, pH7.4, 0.5% BSA

Preservative

0.09% sodium azide

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Epitope

N-terminus

More Infomation

Target

Full Name

Actin, Alpha, Cardiac Muscle 1

Introduction

Actins are highly conserved proteins that are involved in various types of cell motility. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to four others. The prot

Entrez Gene ID

UniProt ID

P68032

Alternative Names

Actin, Alpha, Cardiac Muscle 1; ACTC; Actin, Alpha Cardiac Muscle 1; Actin, Alpha, Cardiac Muscle; Alpha-Cardiac Actin; CMD1R; CMH11; LVNC4; ASD5;

Function

Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.

Biological Process

Actin filament-based movement
Actin filament organization
Actin-myosin filament sliding
Actomyosin structure organization
Cardiac muscle contraction
Cardiac muscle tissue morphogenesis
Cardiac myofibril assembly
Heart contraction
Mesenchyme migration
Muscle filament sliding
Positive regulation of gene expression
Response to drug
Response to ethanol
Skeletal muscle thin filament assembly

Cellular Location

Cytoskeleton

Involvement in disease

Cardiomyopathy, dilated 1R (CMD1R): A disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
Cardiomyopathy, familial hypertrophic 11 (CMH11): A hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death.
Atrial septal defect 5 (ASD5): A congenital heart malformation characterized by incomplete closure of the wall between the atria resulting in blood flow from the left to the right atria.

PTM

Oxidation of Met-46 and Met-49 by MICALs (MICAL1, MICAL2 or MICAL3) to form methionine sulfoxide promotes actin filament depolymerization. MICAL1 and MICAL2 produce the (R)-S-oxide form. The (R)-S-oxide form is reverted by MSRB1 and MSRB2, which promotes actin repolymerization.
Monomethylation at Lys-86 (K84me1) regulates actin-myosin interaction and actomyosin-dependent processes. Demethylation by ALKBH4 is required for maintaining actomyosin dynamics supporting normal cleavage furrow ingression during cytokinesis and cell migration.
Methylated at His-75 by SETD3.
(Microbial infection) Monomeric actin is cross-linked by V.cholerae toxins RtxA and VgrG1 in case of infection: bacterial toxins mediate the cross-link between Lys-52 of one monomer and Glu-272 of another actin monomer, resulting in formation of highly toxic actin oligomers that cause cell rounding. The toxin can be highly efficient at very low concentrations by acting on formin homology family proteins: toxic actin oligomers bind with high affinity to formins and adversely affect both nucleation and elongation abilities of formins, causing their potent inhibition in both profilin-dependent and independent manners.

Suresh, R., Picard, D., Lo, R., Beaulieu, J., Remke, M., & Diaz, R. J. (2021). Expression of cell type incongruent alpha-cardiac actin 1 subunit in medulloblastoma reveals a novel mechanism for cancer cell survival and control of migration. Neuro-Oncology Advances.

Frank, D., Rangrez, A. Y., Friedrich, C., Dittmann, S., Stallmeyer, B., Yadav, P., ... & Schulze-Bahr, E. (2019). Cardiac α-actin (ACTC1) gene mutation causes atrial-septal defects associated with late-onset dilated cardiomyopathy. Circulation: Genomic and Precision Medicine, 12(8), e002491.

Rangrez, A. Y., Kilian, L., Stiebeling, K., Dittmann, S., Schulze-Bahr, E., Frey, N., & Frank, D. (2019). A cardiac α-actin (ACTC1) p. Gly247Asp mutation inhibits SRF-signaling in vitro in neonatal rat cardiomyocytes. Biochemical and biophysical research communications, 518(3), 500-505.

Wanibuchi, M., Ohtaki, S., Ookawa, S., Kataoka-Sasaki, Y., Sasaki, M., Oka, S., ... & Honmou, O. (2018). Actin, alpha, cardiac muscle 1 (ACTC1) knockdown inhibits the migration of glioblastoma cells in vitro. Journal of the neurological sciences, 392, 117-121.

Smith, J. G., Owen, T., Bhagwan, J. R., Mosqueira, D., Scott, E., Mannhardt, I., ... & Denning, C. (2018). Isogenic pairs of hiPSC-CMs with hypertrophic cardiomyopathy/LVNC-associated ACTC1 E99K mutation unveil differential functional deficits. Stem cell reports, 11(5), 1226-1243.

Cheung, A. S., de Rooy, C., Levinger, I., Rana, K., Clarke, M. V., How, J. M., ... & Grossmann, M. (2017). Actin alpha cardiac muscle 1 gene expression is upregulated in the skeletal muscle of men undergoing androgen deprivation therapy for prostate cancer. The Journal of steroid biochemistry and molecular biology, 174, 56-64.

Boutilier, J. K., Taylor, R. L., Ram, R., McNamara, E., Nguyen, Q., Goullée, H., ... & Nowak, K. J. (2017). Variable cardiac α-actin (Actc1) expression in early adult skeletal muscle correlates with promoter methylation. Biochimica et Biophysica Acta (BBA)-Gene Regulatory Mechanisms, 1860(10), 1025-1036.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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