Mouse Anti-ACVR1B Recombinant Antibody (V2-610964) (CBMAB-A0107-LY)

Name: Mouse Anti-ACVR1B Recombinant Antibody (V2-610964)
SKU: CBMAB-A0107-LY
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

V2-610964

Application

WB, ELISA

Immunogen

ACVR1B (AAH00254, 24 a.a. ~ 126 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG1, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, pH7.4

Preservative

None

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Activin A Receptor Type 1B

Introduction

Activins are dimeric growth and differentiation factors which belong to the transforming growth factor-beta (TGF-beta) superfamily of structurally related signaling proteins. Activins signal through a heteromeric complex of receptor serine kinases which include at least two type I (I and IB) and two type II (II and IIB) receptors. These receptors are all transmembrane proteins, composed of a ligand-binding extracellular domain with a cysteine-rich region, a transmembrane domain, and a cytoplasmic domain with predicted serine/threonine specificity. Type I receptors are essential for signaling, and type II receptors are required for binding ligands and for expression of type I receptors. Type I and II receptors form a stable complex after ligand binding, resulting in phosphorylation of type I receptors by type II receptors. This gene encodes activin A type IB receptor, composed of 11 exons. Alternative splicing and alternative polyadenylation result in 3 fully described transcript variants. The mRNA expression of variants 1, 2, and 3 is confirmed, and a potential fourth variant contains an alternative exon 8 and lacks exons 9 through 11, but its mRNA expression has not been confirmed. [provided by RefSeq]

Entrez Gene ID

Alternative Names

ACTRIB; ACVRLK4; ALK4; SKR2

Function

Transmembrane serine/threonine kinase activin type-1 receptor forming an activin receptor complex with activin receptor type-2 (ACVR2A or ACVR2B). Transduces the activin signal from the cell surface to the cytoplasm and is thus regulating a many physiological and pathological processes including neuronal differentiation and neuronal survival, hair follicle development and cycling, FSH production by the pituitary gland, wound healing, extracellular matrix production, immunosuppression and carcinogenesis. Activin is also thought to have a paracrine or autocrine role in follicular development in the ovary. Within the receptor complex, type-2 receptors (ACVR2A and/or ACVR2B) act as a primary activin receptors whereas the type-1 receptors like ACVR1B act as downstream transducers of activin signals. Activin binds to type-2 receptor at the plasma membrane and activates its serine-threonine kinase. The activated receptor type-2 then phosphorylates and activates the type-1 receptor such as ACVR1B. Once activated, the type-1 receptor binds and phosphorylates the SMAD proteins SMAD2 and SMAD3, on serine residues of the C-terminal tail. Soon after their association with the activin receptor and subsequent phosphorylation, SMAD2 and SMAD3 are released into the cytoplasm where they interact with the common partner SMAD4. This SMAD complex translocates into the nucleus where it mediates activin-induced transcription. Inhibitory SMAD7, which is recruited to ACVR1B through FKBP1A, can prevent the association of SMAD2 and SMAD3 with the activin receptor complex, thereby blocking the activin signal. Activin signal transduction is also antagonized by the binding to the receptor of inhibin-B via the IGSF1 inhibin coreceptor. ACVR1B also phosphorylates TDP2.

Biological Process

Activin receptor signaling pathway
Cellular response to growth factor stimulus
Central nervous system development
Development of primary female sexual characteristics
Extrinsic apoptotic signaling pathway
G1/S transition of mitotic cell cycle
Hair follicle development
In utero embryonic development
Negative regulation of cell growth
Negative regulation of gene expression
Nervous system development
Nodal signaling pathway
Peptidyl-threonine phosphorylation
Positive regulation of activin receptor signaling pathway
Positive regulation of erythrocyte differentiation
Positive regulation of pathway-restricted SMAD protein phosphorylation
Positive regulation of transcription by RNA polymerase II
Positive regulation of trophoblast cell migration
Protein autophosphorylation
Protein phosphorylation
Regulation of transcription, DNA-templated
Signal transduction
Transmembrane receptor protein serine/threonine kinase signaling pathway

Cellular Location

Cell membrane

Involvement in disease

ACVRIB is abundantly expressed in systemic sclerosis patient fibroblasts and production of collagen is also induced by activin-A/INHBA. This suggests that the activin/ACRV1B signaling mechanism is involved in systemic sclerosis.

Topology

Extracellular: 24-126 aa
Helical: 127-149 aa
Cytoplasmic: 150-505 aa

PTM

Autophosphorylated. Phosphorylated by activin receptor type-2 (ACVR2A or ACVR2B) in response to activin-binding at serine and threonine residues in the GS domain. Phosphorylation of ACVR1B by activin receptor type-2 regulates association with SMAD7.
Ubiquitinated. Level of ubiquitination is regulated by the SMAD7-SMURF1 complex.

Zheng, J., Zhang, T., Han, S., Liu, C., Liu, M., Li, S., & Li, J. (2021). Activin A improves the neurological outcome after ischemic stroke in mice by promoting oligodendroglial ACVR1B-mediated white matter remyelination. Experimental Neurology, 337, 113574.

Boueiz, A., Pham, B., Chase, R., Lamb, A., Lee, S., Naing, Z. Z. C., ... & Castaldi, P. J. (2019). Integrative genomics analysis identifies ACVR1B as a candidate causal gene of emphysema distribution. American journal of respiratory cell and molecular biology, 60(4), 388-398.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Antibody Pairs

ACVR1B Matched Antibody Pair (018) (CAT#: APMAB-018LY)

ACVR1B Matched Antibody Pair (019) (CAT#: APMAB-019LY)

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Isotype control

For research use only. Not intended for any clinical use.

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