Mouse Anti-ADAM15 Recombinant Antibody (V2-179756) (CBMAB-A1118-YC)

Name: Mouse Anti-ADAM15 Recombinant Antibody (V2-179756)
SKU: CBMAB-A1118-YC
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

V2-179756

Application

WB, IHC, IP, IHC-F, ICC, MC

Immunogen

COS-7 African green monkey SV40 transformed kidney fibroblast-like cell line-derived recombinant human ADAM15, Asp207-Thr696.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG1

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1 μg/ml
IF(ICC)	8-25 μg/ml
IP	25 μg/ml
IHC	8-25 μg/ml
FC	2.5 μg/10^6 cells

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Lyophilized

Buffer

PBS, Trehalose

Preservative

None

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

ADAM Metallopeptidase Domain 15

Introduction

ADAM15 is a member of the ADAM (a disintegrin and metalloproteinase) protein family. ADAM family members are type I transmembrane glycoproteins known to be involved in cell adhesion and proteolytic ectodomain processing of cytokines and adhesion molecules

Entrez Gene ID

8751

UniProt ID

Q13444

Alternative Names

ADAM Metallopeptidase Domain 15; Metalloproteinase-Like, Disintegrin-Like, And Cysteine-Rich Protein 15; A Disintegrin And Metalloproteinase Domain 15 (Metargidin); Metalloprotease RGD Disintegrin Protein; Metargidin; MDC-15;

Function

Active metalloproteinase with gelatinolytic and collagenolytic activity. Plays a role in the wound healing process. Mediates both heterotypic intraepithelial cell/T-cell interactions and homotypic T-cell aggregation. Inhibits beta-1 integrin-mediated cell adhesion and migration of airway smooth muscle cells. Suppresses cell motility on or towards fibronectin possibly by driving alpha-v/beta-1 integrin (ITAGV-ITGB1) cell surface expression via ERK1/2 inactivation. Cleaves E-cadherin in response to growth factor deprivation. Plays a role in glomerular cell migration. Plays a role in pathological neovascularization. May play a role in cartilage remodeling. May be proteolytically processed, during sperm epididymal maturation and the acrosome reaction. May play a role in sperm-egg binding through its disintegrin domain.

Biological Process

Angiogenesis
Cardiac epithelial to mesenchymal transition
Cell-matrix adhesion
Cellular response to phorbol 13-acetate 12-myristate
Collagen catabolic process
Extracellular matrix disassembly
Extracellular matrix organization
Immune response to tumor cell
Innate immune response
Integrin-mediated signaling pathway
Male gonad development
Negative regulation of cell growth
Negative regulation of cell-matrix adhesion
Negative regulation of cell migration
Negative regulation of receptor binding
Response to hypobaric hypoxia
Tissue regeneration

Cellular Location

Endomembrane system; Adherens junction. The majority of the protein is localized in a perinuclear compartment which may correspond to the trans-Golgi network or the late endosome. The pro-protein is the major detectable form on the cell surface, whereas the majority of the protein in the cell is processed (By similarity).

Topology

Extracellular: 207-696 aa
Helical: 697-717 aa
Cytoplasmic: 718-863 aa

PTM

The precursor is cleaved by a furin endopeptidase.
Phosphorylation increases association with PTKs.

Wang, X., Rojas-Quintero, J., Zhang, D., Nakajima, T., Walker, K. H., Peh, H. Y., ... & Owen, C. A. (2021). A disintegrin and metalloproteinase domain-15 deficiency leads to exaggerated cigarette smoke-induced chronic obstructive pulmonary disease (COPD)-like disease in mice. Mucosal Immunology, 14(2), 342-356.

Chute, M. (2021). The Role of Disintegrin and Metalloproteinase 15 (ADAM15) in Myocardial Infarction.

Yang, Q., Pei, R., Wang, Y., Zhou, Y., Yang, M., Chen, X., & Chen, J. (2021). ADAM15 participates in tick-borne encephalitis virus replication. Journal of Virology, 95(4), e01926-20.

Wang, X., Zhang, D., Higham, A., Wolosianka, S., Gai, X., Zhou, L., ... & Owen, C. A. (2020). ADAM15 expression is increased in lung CD8+ T cells, macrophages, and bronchial epithelial cells in patients with COPD and is inversely related to airflow obstruction. Respiratory research, 21(1), 1-17.

Jana, S., Chute, M., Hu, M., Winkelaar, G., Owen, C. A., Oudit, G. Y., & Kassiri, Z. (2020). ADAM (a disintegrin and metalloproteinase) 15 deficiency exacerbates Ang II (angiotensin II)–Induced aortic remodeling leading to abdominal aortic aneurysm. Arteriosclerosis, thrombosis, and vascular biology, 40(8), 1918-1934.

Aujla, P., Jana, S., Chute, M., & Kassiri, Z. (2020, July). Role of a Disintegrin and Metalloproteinase 15 in Cardiac Hypertrophy and Fibrosis Following Pressure Overload. In Circulation Research (Vol. 127, No. Suppl_1, pp. A282-A282). Hagerstown, MD: Lippincott Williams & Wilkins.

Mattern, J., Roghi, C. S., Hurtz, M., Knäuper, V., Edwards, D. R., & Poghosyan, Z. (2019). ADAM15 mediates upregulation of Claudin-1 expression in breast cancer cells. Scientific reports, 9(1), 1-14.

Horowitz, J. D., & Liu, S. (2018). ADAM-15 and glycocalyx shedding: a new perspective on sepsis-related vasomotor dysfunction.

Li, H., Guo, X., Li, Q., Ran, P., Xiang, X., Yuan, Y., ... & Zheng, S. (2018). Long non-coding RNA 1308 promotes cell invasion by regulating the miR-124/ADAM 15 axis in non-small-cell lung cancer cells. Cancer management and research, 10, 6599.

Yang, X., Meegan, J. E., Jannaway, M., Coleman, D. C., & Yuan, S. Y. (2018). A disintegrin and metalloproteinase 15-mediated glycocalyx shedding contributes to vascular leakage during inflammation. Cardiovascular research, 114(13), 1752-1763.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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