Human Recombinant ADAM15 protein, His Tag (V2LY-0526-LY1935)

Name: Human Recombinant ADAM15 protein, His Tag
SKU: V2LY-0526-LY1935
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

HEK293 Cells

Protein Species

Human

Tag

His Tag

Protein Construction

This product is Human Recombinant ADAM15 protein, His Tag consist of Amino Acid: 1-696 and predicts a molecular mass of 54 kDa.

Molecule Mass

54 kDa

Sequence

Amino Acid: 1-696

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>97% as determined by SDS-PAGE

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile This product is Lyophilized from sterile PBS, 5% Trehalose, 5% Mannitol, pH 7.4.
Please contact us for any concerns or special requirements.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

ADAM Metallopeptidase Domain 15

Function

Active metalloproteinase with gelatinolytic and collagenolytic activity. Plays a role in the wound healing process. Mediates both heterotypic intraepithelial cell/T-cell interactions and homotypic T-cell aggregation. Inhibits beta-1 integrin-mediated cell adhesion and migration of airway smooth muscle cells. Suppresses cell motility on or towards fibronectin possibly by driving alpha-v/beta-1 integrin (ITAGV-ITGB1) cell surface expression via ERK1/2 inactivation. Cleaves E-cadherin in response to growth factor deprivation. Plays a role in glomerular cell migration. Plays a role in pathological neovascularization. May play a role in cartilage remodeling. May be proteolytically processed, during sperm epididymal maturation and the acrosome reaction. May play a role in sperm-egg binding through its disintegrin domain.

Biological Process

Angiogenesis
Cardiac epithelial to mesenchymal transition
Cell-matrix adhesion
Cellular response to phorbol 13-acetate 12-myristate
Collagen catabolic process
Extracellular matrix disassembly
Extracellular matrix organization
Immune response to tumor cell
Innate immune response
Integrin-mediated signaling pathway
Male gonad development
Negative regulation of cell growth
Negative regulation of cell-matrix adhesion
Negative regulation of cell migration
Negative regulation of receptor binding
Response to hypobaric hypoxia
Tissue regeneration

Cellular Location

Endomembrane system; Adherens junction. The majority of the protein is localized in a perinuclear compartment which may correspond to the trans-Golgi network or the late endosome. The pro-protein is the major detectable form on the cell surface, whereas the majority of the protein in the cell is processed (By similarity).

Topology

Extracellular: 207-696 aa
Helical: 697-717 aa
Cytoplasmic: 718-863 aa

PTM

The precursor is cleaved by a furin endopeptidase.
Phosphorylation increases association with PTKs.

Wang, X., Rojas-Quintero, J., Zhang, D., Nakajima, T., Walker, K. H., Peh, H. Y., ... & Owen, C. A. (2021). A disintegrin and metalloproteinase domain-15 deficiency leads to exaggerated cigarette smoke-induced chronic obstructive pulmonary disease (COPD)-like disease in mice. Mucosal Immunology, 14(2), 342-356.

Chute, M. (2021). The Role of Disintegrin and Metalloproteinase 15 (ADAM15) in Myocardial Infarction.

Yang, Q., Pei, R., Wang, Y., Zhou, Y., Yang, M., Chen, X., & Chen, J. (2021). ADAM15 participates in tick-borne encephalitis virus replication. Journal of Virology, 95(4), e01926-20.

Wang, X., Zhang, D., Higham, A., Wolosianka, S., Gai, X., Zhou, L., ... & Owen, C. A. (2020). ADAM15 expression is increased in lung CD8+ T cells, macrophages, and bronchial epithelial cells in patients with COPD and is inversely related to airflow obstruction. Respiratory research, 21(1), 1-17.

Jana, S., Chute, M., Hu, M., Winkelaar, G., Owen, C. A., Oudit, G. Y., & Kassiri, Z. (2020). ADAM (a disintegrin and metalloproteinase) 15 deficiency exacerbates Ang II (angiotensin II)–Induced aortic remodeling leading to abdominal aortic aneurysm. Arteriosclerosis, thrombosis, and vascular biology, 40(8), 1918-1934.

Aujla, P., Jana, S., Chute, M., & Kassiri, Z. (2020, July). Role of a Disintegrin and Metalloproteinase 15 in Cardiac Hypertrophy and Fibrosis Following Pressure Overload. In Circulation Research (Vol. 127, No. Suppl_1, pp. A282-A282). Hagerstown, MD: Lippincott Williams & Wilkins.

Mattern, J., Roghi, C. S., Hurtz, M., Knäuper, V., Edwards, D. R., & Poghosyan, Z. (2019). ADAM15 mediates upregulation of Claudin-1 expression in breast cancer cells. Scientific reports, 9(1), 1-14.

Horowitz, J. D., & Liu, S. (2018). ADAM-15 and glycocalyx shedding: a new perspective on sepsis-related vasomotor dysfunction.

Li, H., Guo, X., Li, Q., Ran, P., Xiang, X., Yuan, Y., ... & Zheng, S. (2018). Long non-coding RNA 1308 promotes cell invasion by regulating the miR-124/ADAM 15 axis in non-small-cell lung cancer cells. Cancer management and research, 10, 6599.

Yang, X., Meegan, J. E., Jannaway, M., Coleman, D. C., & Yuan, S. Y. (2018). A disintegrin and metalloproteinase 15-mediated glycocalyx shedding contributes to vascular leakage during inflammation. Cardiovascular research, 114(13), 1752-1763.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human Recombinant ADAM15 protein, His Tag-1 (CAT#: V2LY-0526-LY1936)

Mouse Recombinant ADAM15 protein, His Tag (CAT#: V2LY-0526-LY7780)

Mouse Adam15 ELISA Kit (CAT#: V2LY-0626-LY2315)

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Mouse Adam15 ELISA Kit (CAT#: V2LY-0626-LY2315)

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Mouse Anti-ADAM15 Recombinant Antibody (V2-179755) (CAT#: CBMAB-A1117-YC)

Human Recombinant ADAM15 protein, His Tag-1 (CAT#: V2LY-0526-LY1936)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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