What applications has this antibody been validated for, and what are the recommended working dilutions?

This antibody has been validated for Western Blotting (WB), Immunohistochemistry (IHC), and Flow Cytometry (FACS). While optimal dilutions should be determined by the user, we recommend starting with the following as a guideline: WB: 1:1,000–1:5,000 IHC: 1:200–1:1,000 FACS: 1:200–1:500

What is the expected molecular weight of ADAM19 in a Western blot?

The expected molecular weight of the Rat ADAM19 protein is approximately 105 kDa. However, due to post-translational modifications, the protein may appear as a slightly different size on an SDS-PAGE gel.

Rat Anti-ADAM19 Recombinant Antibody (V2-179769) (CBMAB-A1131-YC)

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Datasheet

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Basic Information

Host Animal

Rat

Clone

V2-179769

Application

Immunogen

Mouse recombinant protein of ADAM19 ectodomain

Host Species

Rat

Specificity

Mouse

Antibody Isotype

IgG2

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:500-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

PBS

Buffer

PBS

Preservative

None

Concentration

0.5 mg/ml

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

ADAM Metallopeptidase Domain 19

Introduction

ADAM19 is a member of the ADAM (a disintegrin and metalloprotease domain) family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins and have been implicated in a variety of biological processes involvin

Entrez Gene ID

11492

UniProt ID

O35674

Alternative Names

ADAM Metallopeptidase Domain 19; A Disintegrin And Metalloproteinase Domain 19 (Meltrin Beta); Metalloprotease And Disintegrin Dendritic Antigen Marker; Meltrin-Beta; ADAM 19; MADDAM; MLTNB;

Function

Participates in the proteolytic processing of beta-type neuregulin isoforms which are involved in neurogenesis and synaptogenesis, suggesting a regulatory role in glial cell. Also cleaves alpha-2 macroglobulin. May be involved in osteoblast differentiation and/or osteoblast activity in bone (By similarity).

Biological Process

Amyloid precursor protein catabolic process
Extracellular matrix organization
Membrane protein ectodomain proteolysis
Placenta development
Positive regulation of cell-cell adhesion mediated by cadherin
Positive regulation of gene expression
Protein processing

Cellular Location

Membrane

Topology

Extracellular: 203-699 aa
Helical: 700-720 aa
Cytoplasmic: 721-955 aa

PTM

The precursor is cleaved by a furin endopeptidase.

Wang, J., Nie, W., Xie, X., Bai, M., Ma, Y., Jin, L., ... & Han, F. (2021). MicroRNA-874-3p/ADAM (A Disintegrin and Metalloprotease) 19 Mediates Macrophage Activation and Renal Fibrosis After Acute Kidney Injury. Hypertension, 77(5), 1613-1626.

Kang, T. Z. E., Zhu, L., Yang, D., Ding, D., Zhu, X., Wan, Y. C. E., ... & Chan, K. M. (2021). The elevated transcription of ADAM19 by the oncohistone H2BE76K contributes to oncogenic properties in breast cancer. Journal of Biological Chemistry, 296.

Li, H., Nichols, C., Gruzdev, A., House, J. S., Ward, J., Li, J., ... & London, S. (2020). ADAM19 and Pulmonary Function in the Mouse: Follow-Up of Human GWAS. In A67. STRUCTURE-FUNCTION RELATIONSHIPS (pp. A2386-A2386). American Thoracic Society.

Xu, S., Huang, S., Li, D., Zou, Q., Yuan, Y., & Yang, Z. (2020). Comparison of ADAM19 and CUEDC2 expression in EHCC and their clinicopathological significance. Biomarkers in Medicine, 14(16), 1573-1584.

Weerasekera, L., Rudnicka, C., Sang, Q. X., Curran, J. E., Johnson, M. P., Moses, E. K., ... & Matthews, V. B. (2017). ADAM19: a novel target for metabolic syndrome in humans and mice. Mediators of inflammation, 2017.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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