Sign in or Register   Sign in or Register
  |  

Mouse Anti-ADAM28 Recombinant Antibody (V2-179785) (CBMAB-A1147-YC)

Provided herein is a Mouse monoclonal antibody against Mouse ADAM Metallopeptidase Domain 28. The antibody can be used for immunoassay techniques, such as WB, IP, IF, ELISA.
See all ADAM28 antibodies
Published Data
Mouse Anti-ADAM28 Recombinant Antibody (H-4) (CBMAB-A1147-YC) in IF
Immunofluorescence staining of BIR cells in breast cancer for CD20 (green) and the indicated markers (red), including ADAM28, CD22 , CD79B, and CD180. ...View More
Wu, Z., Zhou, J., Xiao, Y., Ming, J., Zhou, J., Dong, F., ... & Huang, T. (2022). CD20+ CD22+ ADAM28+ b cells in tertiary lymphoid structures promote immunotherapy response. Frontiers in Immunology, 13.
Mouse Anti-ADAM28 Recombinant Antibody (H-4) (CBMAB-A1147-YC) in FC
Flow cytometry analysis of CD20+CD22+Adam28+ BIR cells in tumor tissues. The heatmap shows a projection of Adam28 expression onto all tumor CD45+CD19+ B cells and the histogram shows the percentage of BIR cells in all CD19+ B cells. ...View More
Wu, Z., Zhou, J., Xiao, Y., Ming, J., Zhou, J., Dong, F., ... & Huang, T. (2022). CD20+ CD22+ ADAM28+ b cells in tertiary lymphoid structures promote immunotherapy response. Frontiers in Immunology, 13.
Mouse Anti-ADAM28 Recombinant Antibody (H-4) (CBMAB-A1147-YC) in WB
ADAM28 protein levels after knockdown or overexpression of miR-128-3p in NSCLC cells were assessed by western blotting (n = 3 three biological replicates; ANOVA followed by Tukey's post-hoc test). ...View More
Xie, Y., Zheng, Z. W., He, H. T., & Chang, Z. B. (2022). LncRNA NEAT1 induces autophagy through the miR-128-3p/ADAM28 axis to suppress apoptosis of nonsmall-cell lung cancer. The Kaohsiung Journal of Medical Sciences, 38(10), 933-949.
Mouse Anti-ADAM28 Recombinant Antibody (H-4) (CBMAB-A1147-YC) in Immunoblot
Elevated ADAM28 expression levels in high fat fed mice. Active ADAM28 (42 kDa) protein levels were measured by immunoblotting (A) and quantified based on densitometry (B) in livers of mice on a high fat diet for 12 weeks * p < 0.05; n = 3-4 mice/group. ...View More
Herat, L., Rudnicka, C., Okada, Y., Mochizuki, S., Schlaich, M., & Matthews, V. (2017). The metalloproteinase ADAM28 promotes metabolic dysfunction in mice. International Journal of Molecular Sciences, 18(4), 884.

Summary

Host Animal
Mouse
Specificity
Mouse
Clone
V2-179785
Antibody Isotype
IgG3, κ
Application
WB, IP, IF, ELISA

Basic Information

Immunogen
Raised against amino acids 143-307 mapping within an internal region of ADAM28 of mouse origin.
Host Species
Mouse
Specificity
Mouse
Antibody Isotype
IgG3, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate).
IF(ICC)1:50-1:500
ELISA1:30-1:3,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Buffer
PBS, 0.1% gelatin.
Preservative
< 0.1% sodium azide
Concentration
0.2 mg/ml
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
ADAM Metallopeptidase Domain 28
Introduction
ADAM28 is a member of the ADAM (a disintegrin and metalloprotease domain) family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biological processes involvi
Entrez Gene ID
13522
UniProt ID
Q9R1V6
Alternative Names
ADAM Metallopeptidase Domain 28; Epididymal Metalloproteinase-Like, Disintegrin-Like, And Cysteine-Rich Protein II; ADAM 28; EMDC II; MDC-L; MDCL; Epididymial Metalloproteinase-Like, Disintegrin-Like, And Cysteine-Rich Protein II; Metalloproteinase-Like,
Function
May play a role in the adhesive and proteolytic events that occur during lymphocyte emigration or may function in ectodomain shedding of lymphocyte surface target proteins, such as FASL and CD40L. May be involved in sperm maturation.
Biological Process
Spermatogenesis
Cellular Location
Isoform 1: Cell membrane
Isoform 2: Secreted
Topology
Extracellular: 199-665 aa
Helical: 666-686 aa
Cytoplasmic: 687-775 aa
PTM
Pro-domain removal and maturation may be, at least in part, autocatalytic.

Zhong, Y., Lin, H., Li, Q., Liu, C., & Shen, J. (2021). CircRNA_100565 contributes to cisplatin resistance of NSCLC cells by regulating proliferation, apoptosis and autophagy via miR-337-3p/ADAM28 axis. Cancer Biomarkers, 30(2), 261-273.

Zha, Z., Jia, F., Hu, P., Mai, E., & Lei, T. (2020). MicroRNA‑574‑3p inhibits the malignant behavior of liver cancer cells by targeting ADAM28. Oncology letters, 20(3), 3015-3023.

Hubeau, C., Rocks, N., & Cataldo, D. (2020). ADAM28: Another ambivalent protease in cancer. Cancer Letters, 494, 18-26.

Li, L. X., Lam, I. H., Liang, F. F., Yi, S. P., Ye, L. F., Wang, J. T., ... & Xu, M. (2019). MiR-198 affects the proliferation and apoptosis of colorectal cancer through regulation of ADAM28/JAK-STAT signaling pathway. Eur Rev Med Pharmacol Sci, 23(4), 1487-1493.

Wei, L., Wen, J. Y., Chen, J., Ma, X. K., Wu, D. H., Chen, Z. H., & Huang, J. L. (2019). Oncogenic ADAM28 induces gemcitabine resistance and predicts a poor prognosis in pancreatic cancer. World journal of gastroenterology, 25(37), 5590.

Mochizuki, S., Shimoda, M., Abe, H., Miyamae, Y., Kuramoto, J., Aramaki-Hattori, N., ... & Okada, Y. (2018). Selective inhibition of ADAM28 suppresses lung carcinoma cell growth and metastasis. Molecular cancer therapeutics, 17(11), 2427-2438.

Gérard, C., Hubeau, C., Carnet, O., Bellefroid, M., Sounni, N. E., Blacher, S., ... & Rocks, N. (2018). Microenvironment-derived ADAM28 prevents cancer dissemination. Oncotarget, 9(98), 37185.

Herat, L., Rudnicka, C., Okada, Y., Mochizuki, S., Schlaich, M., & Matthews, V. (2017). The metalloproteinase ADAM28 promotes metabolic dysfunction in mice. International journal of molecular sciences, 18(4), 884.

Ask a question We look forward to hearing from you.
0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Loading...
Have you used Mouse Anti-ADAM28 Recombinant Antibody (V2-179785)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon $30 eGift Card
Submit a review
Loading...
For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Documents

Contact us

  • Tel: (USA)
  • (UK)
  • Fax:
  • Email:

Submit A Review

Go to
Compare