Human ADAM28 ELISA Kit (V2LY-0626-LY4496)

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Tested Data
Request for COA
Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Sensitivity
0.018 ng/mL
Detection Range
0.05-20 ng/mL
Sample Type
Serum, Plasma, cell culture supernates
Specificity
Human
Assay Type
Sandwich
Reactivity
Human
Assay Time
1.5 h
Molecule Mass
87.1 kDa
Components
  • Pre-coated ELISA Plate: 12 wells * 8 detachable strips
  • Standard solution: 0.5ml x1
  • Standard diluent: 3ml x1
  • Streptavidin-HRP: 6ml x1
  • Stop solution: 6ml x1
  • Substrate solution A: 6ml x1
  • Substrate solution B: 6ml x1
  • Wash buffer concentrate (25x): 20ml x1
  • Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 2-8°C
More Infomation

Target

Full Name
ADAM Metallopeptidase Domain 28
Function
May play a role in the adhesive and proteolytic events that occur during lymphocyte emigration or may function in ectodomain shedding of lymphocyte surface target proteins, such as FASL and CD40L. May be involved in sperm maturation.
Biological Process
Spermatogenesis
Cellular Location
Isoform 1: Cell membrane
Isoform 2: Secreted
Topology
Extracellular: 199-665 aa
Helical: 666-686 aa
Cytoplasmic: 687-775 aa
PTM
Pro-domain removal and maturation may be, at least in part, autocatalytic.

Zhong, Y., Lin, H., Li, Q., Liu, C., & Shen, J. (2021). CircRNA_100565 contributes to cisplatin resistance of NSCLC cells by regulating proliferation, apoptosis and autophagy via miR-337-3p/ADAM28 axis. Cancer Biomarkers, 30(2), 261-273.

Zha, Z., Jia, F., Hu, P., Mai, E., & Lei, T. (2020). MicroRNA‑574‑3p inhibits the malignant behavior of liver cancer cells by targeting ADAM28. Oncology letters, 20(3), 3015-3023.

Hubeau, C., Rocks, N., & Cataldo, D. (2020). ADAM28: Another ambivalent protease in cancer. Cancer Letters, 494, 18-26.

Li, L. X., Lam, I. H., Liang, F. F., Yi, S. P., Ye, L. F., Wang, J. T., ... & Xu, M. (2019). MiR-198 affects the proliferation and apoptosis of colorectal cancer through regulation of ADAM28/JAK-STAT signaling pathway. Eur Rev Med Pharmacol Sci, 23(4), 1487-1493.

Wei, L., Wen, J. Y., Chen, J., Ma, X. K., Wu, D. H., Chen, Z. H., & Huang, J. L. (2019). Oncogenic ADAM28 induces gemcitabine resistance and predicts a poor prognosis in pancreatic cancer. World journal of gastroenterology, 25(37), 5590.

Mochizuki, S., Shimoda, M., Abe, H., Miyamae, Y., Kuramoto, J., Aramaki-Hattori, N., ... & Okada, Y. (2018). Selective inhibition of ADAM28 suppresses lung carcinoma cell growth and metastasis. Molecular cancer therapeutics, 17(11), 2427-2438.

Gérard, C., Hubeau, C., Carnet, O., Bellefroid, M., Sounni, N. E., Blacher, S., ... & Rocks, N. (2018). Microenvironment-derived ADAM28 prevents cancer dissemination. Oncotarget, 9(98), 37185.

Herat, L., Rudnicka, C., Okada, Y., Mochizuki, S., Schlaich, M., & Matthews, V. (2017). The metalloproteinase ADAM28 promotes metabolic dysfunction in mice. International journal of molecular sciences, 18(4), 884.

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For research use only. Not intended for any clinical use.

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