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Mouse Anti-ADAM8 Recombinant Antibody (CBT4000) (V2LY-0625-LY1139)

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Summary

Host Animal
Mouse
Specificity
Human
Clone
CBT4000
Antibody Isotype
IgG2a
Application
IHC, ICC, FC

Basic Information

Immunogen
Purified recombinant fragment of human CD156 (AA: extra 17-156) expressed in E. Coli.
Host Species
Mouse
Specificity
Human
Antibody Isotype
IgG2a
Clonality
Monoclonal Antibody
Application Notes
ApplicationNote
WB1:500-1:2,000
IHC-P1:200-1:1,000
ICC1:200-1:1,000
FC1:200-1:400
ELISA1:10,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS
Preservative
Sodium azide
Concentration
Batch dependent
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
ADAM Metallopeptidase Domain 8
Entrez Gene ID
UniProt ID
Function
Possible involvement in extravasation of leukocytes.
Biological Process
Angiogenesis
Cell-cell adhesion
Cell morphogenesis
Cellular response to hypoxia
Extracellular matrix disassembly
Inflammatory response
Leukocyte migration involved in inflammatory response
Lymphocyte chemotaxis
Negative regulation of neuron apoptotic process
Neutrophil degranulation
Positive regulation of acute inflammatory response
Positive regulation of bone resorption
Positive regulation of cell adhesion
Positive regulation of cellular extravasation
Positive regulation of eosinophil migration
Positive regulation of fibronectin-dependent thymocyte migration
Positive regulation of innate immune response
Positive regulation of MAP kinase activity
Positive regulation of membrane protein ectodomain proteolysis
Positive regulation of neutrophil extravasation
Positive regulation of NF-kappaB transcription factor activity
Positive regulation of protein kinase B signaling
Positive regulation of protein processing
Positive regulation of protein secretion
Positive regulation of T cell differentiation in thymus
Positive regulation of thymocyte apoptotic process
Positive regulation of tumor necrosis factor (ligand) superfamily member 11 production
Regulation of cell-cell adhesion
Cellular Location
Membrane
Topology
Extracellular: 17-655 aa
Helical: 656-676 aa
Cytoplasmic: 677-824 aa
More Infomation

Park, G. B., Choi, S., Yoon, Y. S., & Kim, D. (2021). TrkB/C-induced HOXC6 activation enhances the ADAM8-mediated metastasis of chemoresistant colon cancer cells. Molecular Medicine Reports, 23(6), 1-10.

Awan, T., Babendreyer, A., Wozniak, J., Alvi, A. M., Sterzer, V., Cook, L., ... & Ludwig, A. (2021). Expression of the Metalloproteinase ADAM8 Is Upregulated in Liver Inflammation Models and Enhances Cytokine Release In Vitro. Mediators of inflammation, 2021.

Awan, T., Babendreyer, A., Mahmood Alvi, A., Düsterhöft, S., Lambertz, D., Bartsch, J. W., ... & Ludwig, A. (2021). Expression levels of the metalloproteinase ADAM8 critically regulate proliferation, migration and malignant signalling events in hepatoma cells. Journal of cellular and molecular medicine, 25(4), 1982-1999.

van der Vorst, E. P., & Donners, M. M. (2019). ADAM8 in the cardiovascular system: An innocent bystander with clinical use?. Atherosclerosis, 286, 147-149.

Gao, Y., Yu, X., Zhang, F., & Dai, J. (2019). Propofol inhibits pancreatic cancer progress under hypoxia via ADAM 8. Journal of Hepato‐Biliary‐Pancreatic Sciences, 26(6), 219-226.

Chung, H. W., Kim, J. J., Choi, J. I., Lee, H. R., & Lim, J. B. (2019). A disintegrin and metalloproteinase 8 as a potential blood biomarker for early diagnosis of gastric cancer. Yonsei medical journal, 60(8), 713-719.

Conrad, C., Benzel, J., Dorzweiler, K., Cook, L., Schlomann, U., Zarbock, A., ... & Bartsch, J. W. (2019). ADAM8 in invasive cancers: links to tumor progression, metastasis, and chemoresistance. Clinical Science, 133(1), 83-99.

Polverino, F., Rojas-Quintero, J., Wang, X., Petersen, H., Zhang, L., Gai, X., ... & Owen, C. A. (2018). A disintegrin and metalloproteinase domain-8: a novel protective proteinase in chronic obstructive pulmonary disease. American journal of respiratory and critical care medicine, 198(10), 1254-1267.

Johansson, M. W., Khanna, M., Bortnov, V., Annis, D. S., Nguyen, C. L., & Mosher, D. F. (2017). IL‐5‐stimulated eosinophils adherent to periostin undergo stereotypic morphological changes and ADAM 8‐dependent migration. Clinical & Experimental Allergy, 47(10), 1263-1274.

Aung, W. P. P., Chotjumlong, P., Pata, S., Montreekachon, P., Supanchart, C., Khongkhunthian, S., ... & Krisanaprakornkit, S. (2017). Inducible expression of A Disintegrin and Metalloproteinase 8 in chronic periodontitis and gingival epithelial cells. Journal of periodontal research, 52(3), 582-593.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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