Is this antibody suitable for studying the unique endocytic trafficking of ADCY9 from the plasma membrane to endosomes?

Yes. The clone has been validated for Immunocytochemistry (ICC/IF) and is suitable for visualizing both the membrane-bound and endosomal-localized protein populations. For trafficking studies, the high specificity is crucial to ensure that the detected vesicular staining is genuinely ADCY9 and not an artefact from non-specific binding to the many other membrane- or organelle-associated proteins.

What quality assurance is provided to ensure the recombinant antibody is reliable for detecting low ADCY9 levels in clinical tumor samples?

The recombinant technology ensures high purity (>95%) and minimal batch variation, which translates directly into superior signal-to-noise ratio. For low-expressing targets like ADCY9 in tumor samples, this purity is vital to avoid high background, ensuring the antibody's sensitivity is maximized for accurate quantification in techniques like IHC and Western Blot.

Mouse Anti-ADCY9 Recombinant Antibody (V2-179868) (CBMAB-A1246-YC)

See all ADCY9 antibodies

Compare Online Inquiry

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

V2-179868

Application

WB, ICC, IHC-P, ELISA

Immunogen

Adenylate Cyclase 9

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:500-1:5,000
IF(ICC)	1:50-1:200
IHC	1:50-1:200

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

PBS, pH 7.4, 0.02% sodium azide, 50% glycerol

Buffer

PBS, pH7.4, 50% glycerol

Preservative

0.02% sodium azide

Concentration

1 mg/ml

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Adenylate Cyclase 9

Introduction

Adenylate cyclase is a membrane bound enzyme that catalyses the formation of cyclic AMP from ATP. It is regulated by a family of G protein-coupled receptors, protein kinases, and calcium. The type 9 adenylyl cyclase is a widely distributed adenylyl cyclas

Entrez Gene ID

115

UniProt ID

O60503

Alternative Names

Adenylate Cyclase 9; Adenylate Cyclase Type IX; ATP Pyrophosphate-Lyase 9; Adenylyl Cyclase 9; EC 4.6.1.1; ACIX;

Function

Adenylyl cyclase that catalyzes the formation of the signaling molecule cAMP in response to activation of G protein-coupled receptors. Contributes to signaling cascades activated by CRH (corticotropin-releasing factor), corticosteroids and beta-adrenergic receptors.

Biological Process

Activation of adenylate cyclase activity
Activation of protein kinase A activity
Adenylate cyclase-activating adrenergic receptor signaling pathway
Adenylate cyclase-activating G protein-coupled receptor signaling pathway
Adenylate cyclase-inhibiting G protein-coupled receptor signaling pathway
cAMP biosynthetic process
Cellular response to glucagon stimulus
G protein-coupled receptor signaling pathway
In utero embryonic development
Negative regulation of inflammatory response to antigenic stimulus
Renal water homeostasis
Signal transduction

Cellular Location

Cell membrane

Topology

Cytoplasmic: 1-117 aa
Helical: 118-138 aa
Extracellular: 139-141 aa
Helical: 142-162 aa
Cytoplasmic: 163-171 aa
Helical: 172-192 aa
Extracellular: 193-215 aa
Helical: 216-235 aa
Cytoplasmic: 236-241 aa
Helical: 242-259 aa
Extracellular: 260-280 aa
Helical: 281-301 aa
Cytoplasmic: 302-786 aa
Helical: 787-807 aa
Extracellular: 808-818 aa
Helical: 819-839 aa
Cytoplasmic: 840-867 aa
Helical: 868-888 aa
Extracellular: 889-891 aa
Helical: 892-912 aa
Cytoplasmic: 913-920 aa
Helical: 921-941 aa
Extracellular: 942-975 aa
Helical: 976-996 aa
Cytoplasmic: 997-1353 aa

Rhainds, D., Packard, C. J., Brodeur, M. R., Niesor, E. J., Sacks, F. M., Jukema, J. W., ... & Tardif, J. C. (2021). Role of Adenylate Cyclase 9 in the Pharmacogenomic Response to Dalcetrapib: Clinical Paradigm and Molecular Mechanisms in Precision Cardiovascular Medicine. Circulation: Genomic and Precision Medicine, 14(2), e003219.

Rautureau, Y., Berlatie, M., Rivas, D., Uy, K., Miquel, G., Higgins, M. E., ... & Tardif, J. C. (2020). Adenylate Cyclase Type 9 Controls Endothelial Cell Function by Regulation of Cyclic Adenosine Monophosphate and Calcium Signaling Pathways. Arteriosclerosis, Thrombosis, and Vascular Biology, 40(Suppl_1), A561-A561.

Rautureau, Y., Berlatie, M., Rivas, D., Uy, K., Miquel, G., Higgins, M. E., ... & Tardif, J. C. (2019). P719 Endothelial expression of adenylate cyclase type 9 (Adcy9) regulates endothelial cell signalling and vasodilation. European Heart Journal, 40(Supplement_1), ehz747-0324.

Yi, H., Wang, K., Jin, J. F., Jin, H., Yang, L., Zou, Y., ... & Liu, X. (2018). Elevated adenylyl cyclase 9 expression is a potential prognostic biomarker for patients with colon cancer. Medical science monitor: international medical journal of experimental and clinical research, 24, 19.

Rautureau, Y., Deschambault, V., Higgins, M. È., Rivas, D., Mecteau, M., Geoffroy, P., ... & Tardif, J. C. (2018). ADCY9 (adenylate cyclase type 9) inactivation protects from atherosclerosis only in the absence of CETP (cholesteryl ester transfer protein). Circulation, 138(16), 1677-1692.

Rautureau, Y., Deschambault, V., Brand, G., Higgins, M. E., Mecteau, M., Uy, K., ... & Tardif, J. C. (2017). Adenylate Cyclase Type 9 Promotes Atherosclerosis in Mice. Arteriosclerosis, Thrombosis, and Vascular Biology, 37(suppl_1), A184-A184.

Rautureau, Y., Pouliot, P., Higgins, M. È., Geoffroy, P., Suarez, M. L., Rivas, D., ... & Tardif, J. C. (2017). Adenylate Cyclase Type 9 Regulates Body Weight and Adipose Tissue. Circulation, 136(suppl_1), A15956-A15956.

Ask a question We look forward to hearing from you.

0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Have you used Mouse Anti-ADCY9 Recombinant Antibody (V2-179868)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon

$30 eGift Card
Submit a review

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Request bulk or custom quote Second antibody and isotype control

Contact us

Tel: (USA)
(UK)
Fax:

Global Locations

Email:

Online Inquiry