What is the specific species reactivity, and does this antibody cross-react with the closely related human adhesion GPCRs ADGRE2 or CD97?

This antibody is highly specific for Human ADGRE3 (EMR3). Cross-reactivity with the closely related human ADGRE2 or CD97 (ADGRE5) is rigorously screened against. The recombinant design targets unique sequences within the EGF-like domains of ADGRE3, ensuring it selectively marks granulocytes and monocytes in human samples without labeling off-target ADGRE receptors.

What cell type/tissue is recommended as a positive control for ADGRE3 expression in WB or FCM?

ADGRE3 is restricted to human myeloid cells. Recommended positive controls include Human Peripheral Blood Neutrophils, Monocyte-derived Macrophages, or bone marrow myeloid cells. For WB, lysate from granulocyte-enriched PBMCs is highly effective. For IHC, human spleen or tonsil tissue sections serve as good controls due to their high concentration of immune cells.

Mouse Anti-ADGRE3 (AA 1-652) Recombinant Antibody (V2-43346) (CBMAB-E0249-FY)

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Datasheet

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Basic Information

Host Animal

Mouse

Clone

V2-43346

Application

IHC-F, MC

Immunogen

NS0 mouse myeloma cell line transfected with human EMR3, Met1-Tyr652

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG2b

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
FC	0.25 μg/10^6 cells

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Lyophilized

Buffer

PBS, Trehalose

Preservative

None

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Epitope

AA 1-652

More Infomation

Target

Full Name

Adhesion G Protein-Coupled Receptor E3

Introduction

This gene encodes a member of the class B seven-span transmembrane (TM7) receptor family expressed predominantly by cells of the immune system. Family members are characterized by an extended extracellular region with a variable number of N-terminal epidermal growth factor (EGF)-like domains coupled to a TM7 domain via a mucin-like spacer domain. This gene is closely linked to the gene encoding egf-like molecule containing mucin-like hormone receptor 2 on chromosome 19. This protein may play a role in myeloid-myeloid interactions during immune and inflammatory responses. Alternative splicing results in multiple transcript variants encoding different isoforms.

Entrez Gene ID

84658

UniProt ID

Q9BY15

Alternative Names

Adhesion G Protein-Coupled Receptor E3; Egf-Like Module Containing, Mucin-Like, Hormone Receptor-Like 3; EGF-Like Module-Containing Mucin-Like Hormone Receptor-Like 3; EGF-Like Module Receptor 3; EMR3; Egf-Like Module-Containing Mucin-Like Receptor 3

Function

Orphan receptor that may play a role myeloid-myeloid interactions during immune and inflammatory responses. A ligand for the soluble form of this receptor is present at the surface of monocytes-derived macrophages and activated neutrophils.

Biological Process

Adenylate cyclase-activating G protein-coupled receptor signaling pathway
Cell surface receptor signaling pathway
G protein-coupled receptor signaling pathway
Neutrophil degranulation

Cellular Location

Cell membrane
Isoform 3: Secreted

Topology

Extracellular: 22-357 aa
Helical: 358-378 aa
Cytoplasmic: 379-389 aa
Helical: 390-410 aa
Extracellular: 411-416 aa
Helical: 417-437 aa
Cytoplasmic: 438-464 aa
Helical: 465-485 aa
Extracellular: 486-508 aa
Helical: 509-529 aa
Cytoplasmic: 530-557 aa
Helical: 558-578 aa
Extracellular: 579-580 aa
Helical: 581-601 aa
Cytoplasmic: 602-652 aa

PTM

Proteolytically cleaved into 2 subunits, an extracellular alpha subunit and a seven-transmembrane subunit.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

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