Mouse Anti-ADGRL1 Recombinant Antibody (V2-58518) (CBMAB-L0165-YJ)

Provided herein is a Mouse monoclonal antibody, which binds to Adhesion G Protein-Coupled Receptor L1 (ADGRL1). The antibody can be used for immunoassay techniques, such as WB, IP, IF, ELISA.
See all ADGRL1 antibodies

Datasheet

Summary

Host Animal

Mouse

Specificity

Human

Clone

V2-58518

Antibody Isotype

IgG1, κ

Application

WB, IP, IF, ELISA

Basic Information

Immunogen

Specific for an epitope mapping between amino acids 335-357 within an N-terminal extracellular domain of Latrophilin-1 of human origin.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG1, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate).
IF(ICC)	1:50-1:500
ELISA	1:30-1:3,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Buffer

PBS, 0.1% gelatin.

Preservative

< 0.1% sodium azide

Concentration

0.2 mg/ml

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name

Adhesion G Protein-Coupled Receptor L1

Introduction

ADGRL1 is a member of the latrophilin subfamily of G-protein coupled receptors (GPCR). Latrophilins may function in both cell adhesion and signal transduction. In experiments with non-human species, endogenous proteolytic cleavage within a cysteine-rich GPS (G-protein-coupled-receptor proteolysis site) domain resulted in two subunits (a large extracellular N-terminal cell adhesion subunit and a subunit with substantial similarity to the secretin/calcitonin family of GPCRs) being non-covalently bound at the cell membrane. Latrophilin-1 has been shown to recruit the neurotoxin from black widow spider venom, alpha-latrotoxin, to the synapse plasma membrane. Alternative splicing results in multiple variants encoding distinct isoforms.

Entrez Gene ID

22859

UniProt ID

O94910

Alternative Names

Adhesion G Protein-Coupled Receptor L1; Calcium-Independent Alpha-Latrotoxin Receptor 1; Latrophilin-1; Lectomedin-2; CIRL-1; LPHN1

Function

Calcium-independent receptor of high affinity for alpha-latrotoxin, an excitatory neurotoxin present in black widow spider venom which triggers massive exocytosis from neurons and neuroendocrine cells. Receptor for TENM2 that mediates heterophilic synaptic cell-cell contact and postsynaptic specialization. Receptor probably implicated in the regulation of exocytosis (By similarity).

Biological Process

Adenylate cyclase-activating G protein-coupled receptor signaling pathway
Calcium-mediated signaling using intracellular calcium
Cell surface receptor signaling pathway
G protein-coupled receptor signaling pathway
Heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules
Positive regulation of synapse maturation

Cellular Location

Cell membrane; Presynaptic cell membrane; Axon; Growth cone; Synapse; Synaptosome. Colocalizes with TENM2 on the cell surface, across intercellular junctions and on nerve terminals near synaptic clefts.

Topology

Extracellular: 25-858 aa
Helical: 859-879 aa
Cytoplasmic: 880-893 aa
Helical: 894-914 aa
Extracellular: 915-920 aa
Helical: 921-941 aa
Cytoplasmic: 942-964 aa
Helical: 965-985 aa
Extracellular: 986-1002 aa
Helical: 1003-1023 aa
Cytoplasmic: 1024-1050 aa
Helical: 1051-1071 aa
Extracellular: 1072-1075 aa
Helical: 1076-1096 aa
Cytoplasmic: 1097-1474 aa

PTM

Autoproteolytically cleaved into 2 subunits, an extracellular subunit and a seven-transmembrane subunit. This proteolytic processing takes place early in the biosynthetic pathway, either in the endoplasmic reticulum or in the early compartment of the Golgi apparatus (By similarity).

References

Ovando‐Zambrano, J. C., Arias‐Montaño, J. A., & Boucard, A. A. (2019). Alternative splicing event modifying ADGRL1/latrophilin‐1 cytoplasmic tail promotes both opposing and dual cAMP signaling pathways. Annals of the New York Academy of Sciences, 1456(1), 168-185.

Nazarko, O., Kibrom, A., Winkler, J., Leon, K., Stoveken, H., Salzman, G., ... & Araç, D. (2018). A comprehensive mutagenesis screen of the adhesion GPCR latrophilin-1/ADGRL1. Iscience, 3, 264-278.

Rahman, M. A., Manser, C., Benlaouer, O., Suckling, J., Blackburn, J. K., Silva, J., & Ushkaryov, Y. A. (2019). C-terminal phosphorylation of latrophilin-1/ADGRL1 affects the interaction between its fragments. The Annals of the New York Academy of Sciences, 1456(1), 122-143.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Associated Products

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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